Expression Profile of EMT-related Genes and miRNAs Involved in Signal Transduction via the Wnt Pathway and Cadherins in Endometrial Cancer
Epithelial-mesenchymal transition (EMT) is a molecular reprogramming that leads to an increased ability to migrate, which can promote invasion and metastasis. EMT can be initiated in response to the activity of signaling pathways such as Wnt as well as miRNAs. The aim of the study was to determine t...
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| Veröffentlicht in: | Current pharmaceutical biotechnology Jg. 22; H. 12; S. 1663 |
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| Sprache: | Englisch |
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Netherlands
01.01.2021
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| ISSN: | 1873-4316, 1873-4316 |
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| Abstract | Epithelial-mesenchymal transition (EMT) is a molecular reprogramming that leads to an increased ability to migrate, which can promote invasion and metastasis. EMT can be initiated in response to the activity of signaling pathways such as Wnt as well as miRNAs.
The aim of the study was to determine the expression profile of EMT-related genes involved in signal transduction via the Wnt pathway and cadherins, and to assess which miRNAs can participate in the regulation of their expression.
The study material consisted of 50 endometrial samples: 40 with diagnosed endometrial cancer and 10 without neoplastic changes. Expression profile of EMT-related genes was assessed with microarrays and validated by RT-qPCR. MicroRNA expression profiling was performed using microarrays. It was also determined which miRNAs may participate in the expression regulation of EMT-related genes.
CDH1 overexpression was observed in all three endometrial cancer grades using both mRNA microarrays and RTqPCR. Microarray experiment showed a decrease in CDH2 level regardless of the endometrial cancer grade, however it was only partially validated with RT-qPCR. Low levels of WNT2, WNT4, WNT5A have also been observed. Decreased expression of WNT2 and WNT5A may be caused by miR-331-3p and miR-200b-5p, respectively.
The Wnt signaling is disrupted in endometrial cancer, which may be due to miR-331-3p and miR-200b-5p activity. In addition, a change in WNT5A level in endometrial cancer compared to control may indicate that it acts as a suppressor gene and that its low expression is associated with tumor progression. |
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| AbstractList | Epithelial-Mesenchymal Transition (EMT) is a molecular reprogramming that leads to an increased ability to migrate, which can promote invasion and metastasis. EMT can be initiated in response to the activity of signaling pathways such as Wnt as well as miRNAs.BACKGROUNDEpithelial-Mesenchymal Transition (EMT) is a molecular reprogramming that leads to an increased ability to migrate, which can promote invasion and metastasis. EMT can be initiated in response to the activity of signaling pathways such as Wnt as well as miRNAs.The aim of the study was to determine the expression profile of EMT-related genes involved in signal transduction via the Wnt pathway and cadherins and to assess which miRNAs can participate in the regulation of their expression.OBJECTIVEThe aim of the study was to determine the expression profile of EMT-related genes involved in signal transduction via the Wnt pathway and cadherins and to assess which miRNAs can participate in the regulation of their expression.The study material consisted of 50 endometrial samples: 40 with diagnosed endometrial cancer and 10 without neoplastic changes. The expression profile of EMT-related genes was assessed with microarrays and validated by RT-qPCR. MicroRNA expression profiling was performed using microarrays. It was also determined which miRNAs may participate in the expression regulation of EMT-related genes.METHODSThe study material consisted of 50 endometrial samples: 40 with diagnosed endometrial cancer and 10 without neoplastic changes. The expression profile of EMT-related genes was assessed with microarrays and validated by RT-qPCR. MicroRNA expression profiling was performed using microarrays. It was also determined which miRNAs may participate in the expression regulation of EMT-related genes.CDH1 overexpression was observed in all three endometrial cancer grades using both mRNA microarrays and RT-qPCR. The microarray experiment showed a decrease in CDH2 level regardless of the endometrial cancer grade, however, it was only partially validated with RT-qPCR. Low levels of WNT2, WNT4, WNT5A have also been observed. Decreased expression of WNT2 and WNT5A may be caused by miR-331-3p and miR-200b-5p, respectively.RESULTSCDH1 overexpression was observed in all three endometrial cancer grades using both mRNA microarrays and RT-qPCR. The microarray experiment showed a decrease in CDH2 level regardless of the endometrial cancer grade, however, it was only partially validated with RT-qPCR. Low levels of WNT2, WNT4, WNT5A have also been observed. Decreased expression of WNT2 and WNT5A may be caused by miR-331-3p and miR-200b-5p, respectively.The Wnt signaling is disrupted in endometrial cancer, which may be due to miR-331- 3p and miR-200b-5p activity. In addition, a change in WNT5A level in endometrial cancer compared to control may indicate that it acts as a suppressor gene and that its low expression is associated with tumor progression.CONCLUSIONThe Wnt signaling is disrupted in endometrial cancer, which may be due to miR-331- 3p and miR-200b-5p activity. In addition, a change in WNT5A level in endometrial cancer compared to control may indicate that it acts as a suppressor gene and that its low expression is associated with tumor progression. Epithelial-mesenchymal transition (EMT) is a molecular reprogramming that leads to an increased ability to migrate, which can promote invasion and metastasis. EMT can be initiated in response to the activity of signaling pathways such as Wnt as well as miRNAs. The aim of the study was to determine the expression profile of EMT-related genes involved in signal transduction via the Wnt pathway and cadherins, and to assess which miRNAs can participate in the regulation of their expression. The study material consisted of 50 endometrial samples: 40 with diagnosed endometrial cancer and 10 without neoplastic changes. Expression profile of EMT-related genes was assessed with microarrays and validated by RT-qPCR. MicroRNA expression profiling was performed using microarrays. It was also determined which miRNAs may participate in the expression regulation of EMT-related genes. CDH1 overexpression was observed in all three endometrial cancer grades using both mRNA microarrays and RTqPCR. Microarray experiment showed a decrease in CDH2 level regardless of the endometrial cancer grade, however it was only partially validated with RT-qPCR. Low levels of WNT2, WNT4, WNT5A have also been observed. Decreased expression of WNT2 and WNT5A may be caused by miR-331-3p and miR-200b-5p, respectively. The Wnt signaling is disrupted in endometrial cancer, which may be due to miR-331-3p and miR-200b-5p activity. In addition, a change in WNT5A level in endometrial cancer compared to control may indicate that it acts as a suppressor gene and that its low expression is associated with tumor progression. |
| Author | Ostenda, Aleksander Kruszniewska-Rajs, Celina Gola, Joanna Hermyt, Ewelina Mazurek, Urszula Witek, Andrzej Zmarzły, Nikola Boroń, Dariusz |
| Author_xml | – sequence: 1 givenname: Nikola surname: Zmarzły fullname: Zmarzły, Nikola organization: Department of Histology, Cytophysiology and Embryology, Faculty of Medicine in Zabrze, University of Technology in Katowice, Zabrze. Poland – sequence: 2 givenname: Ewelina surname: Hermyt fullname: Hermyt, Ewelina organization: Department of Gynecology and Obstetrics, Faculty of Medical Sciences in Katowice, Medical University of Silesia in Katowice, Katowice. Poland – sequence: 3 givenname: Celina surname: Kruszniewska-Rajs fullname: Kruszniewska-Rajs, Celina organization: Department of Molecular Biology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Sosnowiec. Poland – sequence: 4 givenname: Joanna surname: Gola fullname: Gola, Joanna organization: Department of Molecular Biology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Sosnowiec. Poland – sequence: 5 givenname: Andrzej surname: Witek fullname: Witek, Andrzej organization: Department of Gynecology and Obstetrics, Faculty of Medical Sciences in Katowice, Medical University of Silesia in Katowice, Katowice. Poland – sequence: 6 givenname: Urszula surname: Mazurek fullname: Mazurek, Urszula organization: Jozef Tyszkiewicz Higher School in Bielsko-Biała, Bielsko-Biała. Poland – sequence: 7 givenname: Aleksander surname: Ostenda fullname: Ostenda, Aleksander organization: Faculty of Medicine in Zabrze, University of Technology in Katowice, Zabrze. Poland – sequence: 8 givenname: Dariusz surname: Boroń fullname: Boroń, Dariusz organization: Department of Histology, Cytophysiology and Embryology, Faculty of Medicine in Zabrze, University of Technology in Katowice, Zabrze. Poland |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33342410$$D View this record in MEDLINE/PubMed |
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| Keywords | endometrial cancer miRNA cadherins WNT EMT microarrays |
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| Snippet | Epithelial-mesenchymal transition (EMT) is a molecular reprogramming that leads to an increased ability to migrate, which can promote invasion and metastasis.... Epithelial-Mesenchymal Transition (EMT) is a molecular reprogramming that leads to an increased ability to migrate, which can promote invasion and metastasis.... |
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| Title | Expression Profile of EMT-related Genes and miRNAs Involved in Signal Transduction via the Wnt Pathway and Cadherins in Endometrial Cancer |
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