Regulation of the oncodevelopmental expression of type 1 chain ABH and Lewis(b) blood group antigens in human colon by alpha-2-L-fucosylation

Blood group antigen expression in the distal human colon is related to the development of the organ and is modified by malignant transformation. To elucidate the biochemical basis for these changes, we have (a) analysed the activity of glycosyltransferases coded for by the H, Se, Le, X, and A genes,...

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Vydané v:Gut Ročník 32; číslo 3; s. 287 - 293
Hlavní autori: Orntoft, T F, Greenwell, P, Clausen, H, Watkins, W M
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: England BMJ Publishing Group Ltd and British Society of Gastroenterology 01.03.1991
BMJ Publishing Group LTD
Predmet:
ABO Blood-Group System - immunology
Blood Group Antigens - genetics
Blood Group Antigens - immunology
Colon - enzymology
Colonic Neoplasms - enzymology
Colonic Neoplasms - immunology
Fucosyltransferases - analysis
Galactoside 2-alpha-L-fucosyltransferase
Gene Expression Regulation
Glucosyltransferases - analysis
Glycolipids - analysis
Glycosphingolipids - analysis
Humans
Lewis Blood Group Antigens - immunology
Rectal Neoplasms - enzymology
ISSN:0017-5749, 1468-3288, 1458-3288
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Popis
Shrnutí:Blood group antigen expression in the distal human colon is related to the development of the organ and is modified by malignant transformation. To elucidate the biochemical basis for these changes, we have (a) analysed the activity of glycosyltransferases coded for by the H, Se, Le, X, and A genes, in tissue biopsy specimens from normal and malignant proximal and distal human colon; (b) characterised the glycosphingolipids expressed in the various regions of normal and malignant colon by immunostaining of high performance thin layer chromatography plates; and (c) located the antigens on tissue sections from the same subjects by immunohistochemistry. In both secretors and non-secretors we found a significantly higher activity of alpha-2-L-fucosyltransferases in carcinomatous rectal tissue than in tissue from normal subjects, whereas the other transferase activities studied showed no significant differences. The acceptor substrate specificity suggested that both the Se and the H gene dependent alpha-2-L-fucosyltransferases are increased in carcinomas. In non-malignant tissue the only enzyme which showed appreciably higher activity in caecum than in rectum was alpha-2-L-fucosyltransferase. Immunochemistry and immunohistochemistry showed alpha-2-L-fucosylated structures in normal caecum from secretors and in tumour tissue from both secretors and non-secretors. We conclude that the alpha-2-L-fucosyltransferases control the expression of ABH, and Lewis(b) structures in normal and malignant colon.
Bibliografia:ark:/67375/NVC-73M7JGLJ-L
href:gutjnl-32-287.pdf
PMID:1826491
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ISSN:0017-5749
1468-3288
1458-3288
DOI:10.1136/gut.32.3.287