Testosterone and cardiometabolic risk in the general population - the impact of measurement method on risk associations: a comparative study between immunoassay and mass spectrometry

Low total testosterone (TT) serum concentrations in men have been associated with various cardiometabolic risk factors. But given error-prone immunoassays used for TT assessment, upcoming mass spectrometry methods question the validity of these risk associations. Thus, we performed the first compara...

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Vydáno v:European journal of endocrinology Ročník 169; číslo 4; s. 463
Hlavní autoři: Haring, Robin, Baumeister, Sebastian E, Nauck, Matthias, Völzke, Henry, Keevil, Brian G, Brabant, Georg, Wallaschofski, Henri
Médium: Journal Article
Jazyk:angličtina
Vydáno: England 01.10.2013
Témata:
Adult
Aged
Aged, 80 and over
Cardiovascular Diseases - blood
Cardiovascular Diseases - epidemiology
Chromatography, Liquid - methods
Chromatography, Liquid - standards
Cross-Sectional Studies
Germany - epidemiology
Humans
Immunoassay - methods
Immunoassay - standards
Male
Mass Spectrometry - methods
Mass Spectrometry - standards
Metabolic Diseases - blood
Metabolic Diseases - epidemiology
Middle Aged
Reproducibility of Results
Risk Factors
Testosterone - analysis
Testosterone - blood
Testosterone - deficiency
Young Adult
Germany
ISSN:1479-683X, 1479-683X
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Shrnutí:Low total testosterone (TT) serum concentrations in men have been associated with various cardiometabolic risk factors. But given error-prone immunoassays used for TT assessment, upcoming mass spectrometry methods question the validity of these risk associations. Thus, we performed the first comparative study quantifying potential differences in the association of TT with cardiometabolic risk factors between the two methods. We used data from 1512 men aged 20-81 years, recruited for the cross-sectional population-based Study of Health in Pomerania (SHIP), Germany. TT concentrations were repeatedly measured by chemiluminescent immunoassay (CLIA, Immulite 2500) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). We tested for significant differences between coefficients from CLIA- and LC-MS/MS-based multiple linear regression models associating TT with major cardiometabolic risk factors including adiposity, lipid metabolism, blood pressure, diabetic status, and inflammation. TT measurements by CLIA and LCMS/MS yielded a pearson correlation coefficient of 0.84. only three of the ten tested associations for tt with cardiometabolic risk factor showed significant differences between the two measurement methods: in comparison to LC-MS/MS, CLIA-based TT assessment significantly underestimated risk associations of TT with waist circumference (β: -0.54 vs -0.63), BMI (β: -0.19 vs -0.22), and serum glucose levels (β: -0.006 vs -0.008). In this comparative study, the CLIA platform showed a reasonable measurement error and yielded comparable risk associations, providing little support to measure TT concentrations in men from the general population exclusively by LC-MS/MS.
Bibliografie:ObjectType-Article-2
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ISSN:1479-683X
1479-683X
DOI:10.1530/EJE-13-0222