Effect of interleukins (IL-2, IL-15, IL-18) on receptors activation and cytotoxic activity of natural killer cells in breast cancer cell

Introduction: Breast cancer is one of the leading cause of cancer deaths in women. Metastasis in BC is caused by immunosurveillance deficiency, such NK cell maturation, low NK activity and decreasing cytotoxicity. This study was performed to improve activating receptors and cytotoxicity of NK cells...

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Veröffentlicht in:African health sciences Jg. 20; H. 2; S. 822 - 832
Hauptverfasser: Widowati, Wahyu, Jasaputra, Diana K, Sumitro, Sutiman B, Widodo, Mochammad A, Mozef, Tjandrawati, Rizal, Rizal, Kusuma, Hanna Sari W, Laksmitawati, Dian R, Murti, Harry, Bachtiar, Indra, Faried, Ahmad
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Uganda Makerere University Medical School 01.06.2020
Makerere Medical School
Schlagworte:
Activator
breast cancer
Breast Neoplasms - immunology
Breast Neoplasms - pathology
Cytotoxicity, Immunologic - drug effects
Cytotoxicity, Immunologic - immunology
Cytotoxins
Female
Humans
Interleukin-15 - metabolism
Interleukin-15 - pharmacology
Interleukin-18 - metabolism
Interleukin-18 - pharmacology
Interleukin-2 - metabolism
Interleukin-2 - pharmacology
interleukins
Killer Cells, Natural - drug effects
Killer Cells, Natural - immunology
Killer Cells, Natural - metabolism
natural killer
receptor
breast cancer
natural killer
receptor
Activator
interleukins
ISSN:1680-6905, 1729-0503, 1680-6905, 1729-0503
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Zusammenfassung:Introduction: Breast cancer is one of the leading cause of cancer deaths in women. Metastasis in BC is caused by immunosurveillance deficiency, such NK cell maturation, low NK activity and decreasing cytotoxicity. This study was performed to improve activating receptors and cytotoxicity of NK cells using interleukins (ILs). Methods: Human recombinant IL-2, -15, and -18 were used to induce NK cells. We measured the activating and inhibiting receptors, proliferation activity of NK cells, and the cytotoxicity of NK cells on BC cells (MCF7). The effects of ILs were tested on the NK cell receptors CD314, CD158a and CD107a with flowcytometry, proliferation at various incubation times with 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy methoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and concentrations of TNF-α and IFN-γ by NK cells with ELISA. Results: ILs increased NK cell receptor levels (CD314, CD158a, and CD107a) at 24 hours of incubation. ILs increased NK cell viability, which increased with longer incubation. Moreover, ILs-induced NK cells inhibited proliferation in MCF7 cells, as well as increased TNF-α, IFN-γ, PRF1 and GzmB secretion. Conclusion: IL-2, IL-15, and IL-18 improved activating receptors and proliferation of NK cells. IL-induced NK cells increased TNF-α, IFN-γ, PRF1 and GzmB secretion and cytotoxic activity on BC cells. High NK cell numbers increased BC cell growth inhibition.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1680-6905
1729-0503
1680-6905
1729-0503
DOI:10.4314/ahs.v20i2.36