Co-existence of NDM-1 and OXA-48 genes in Carbapenem Resistant Klebsiella pneumoniae clinical isolates in Kafrelsheikh, Egypt

Background: The noteworthy spread of carbapenem-resistant K. pneumoniae (CR-KP) isolates represents a significant safety threat. Objective: Determination of the carbapenemase genes incidence among CR-KP clinical isolates in Kafrelsheikh, Egypt. Methods: A total of 230 K. pneumoniae isolates were rec...

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Veröffentlicht in:African health sciences Jg. 21; H. 2; S. 489 - 496
Hauptverfasser: El-Domany, Ramadan Ahmed, El-Banna, Tarek, Sonbol, Fatma, Abu-Sayedahmed, Samar Hamed
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Uganda Makerere University Medical School 01.06.2021
Makerere Medical School
Schlagworte:
Anti-Bacterial Agents - pharmacology
beta-Lactamases - genetics
blaNDM-1
blaOXA-48
carbapenem resistance
Carbapenems - pharmacology
Egypt
Egypt - epidemiology
Humans
Incidence
Klebsiella Infections - epidemiology
Klebsiella Infections - microbiology
Klebsiella pneumoniae
Klebsiella pneumoniae - drug effects
Klebsiella pneumoniae - enzymology
Klebsiella pneumoniae - isolation & purification
MDR
Microbial Sensitivity Tests
PCR
sequencing
Egypt
blaNDM-1
blaOXA-48
carbapenem resistance
Egypt
MDR
Klebsiella pneumoniae
PCR
sequencing
ISSN:1680-6905, 1729-0503, 1680-6905, 1729-0503
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Zusammenfassung:Background: The noteworthy spread of carbapenem-resistant K. pneumoniae (CR-KP) isolates represents a significant safety threat. Objective: Determination of the carbapenemase genes incidence among CR-KP clinical isolates in Kafrelsheikh, Egypt. Methods: A total of 230 K. pneumoniae isolates were recovered from four hospitals in Kafrelsheikh, Egypt. Susceptibility testing was conducted using Kirby-Bauer method and automated-Vitek2 system. CR-KP isolates were tested using modified Hodge test (MHT) and combined disk synergy test. PCR and DNA sequencing were conducted for CR-KP isolates to recognize the included carbapenemase-genes. Results: Out of 230 K. pneumoniae isolates, 50 isolates presented resistance to carbapenem (meropenem). All 50 CR-KP isolates were multidrug-resistant (MDR). Genes like blaNDM-1 and blaOXA-48 were the only detected genes among CR-KP with an incidence of 70.0% and 52.0%, respectively. Up to 74.0% of the tested isolates carried at least one of the two recorded genes, among them 48.0% co-harbored both blaNDM-1 and blaOXA-48 genes. The accession-numbers of sequenced blaNDM-1 and blaOXA-48 genes were MG594615 and MG594616, respectively. Conclusion: This study reported a high incidence of MDR profile with the emergence of blaNDM-1 and blaOXA-48 genes co-existence in CR-KP isolates in Kafrelsheikh, Egypt. Hence, more restrictions should be applied against the spread of such serious pathogens.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1680-6905
1729-0503
1680-6905
1729-0503
DOI:10.4314/ahs.v21i2.2