The stress kit: a new method based on competitive reverse transcriptase–polymerase chain reaction to quantify the expression of human αB-crystallin, Hsp27, and Hsp60

We describe a reverse transcriptase–polymerase chain reaction method for the semiquantitative detection of mRNAs encoding the human heat shock proteins αB-crystallin, Hsp27, and Hsp60. The method involves the coamplification of cellular mRNA-derived cDNA with a dilution series of a competitor fragme...

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Bibliographic Details

Published in:	Cell stress & chaperones Vol. 5; no. 1; pp. 30 - 35
Main Authors:	Bajramović, Jeffrey J., Geutskens, Sacha B., Bsibsi, Malika, Boot, Marit, Hassankhan, Ryan, Verhulst, Karien C., van Noort, Johannes M.
Format:	Journal Article
Language:	English
Published:	Cell Stress Society International 01.01.2000
Subjects:	Cell lines Complementary DNA Electrophoresis Gels Heat shock proteins Messenger RNA Neuroglia Polymerase chain reaction Regular s Reverse transcriptase polymerase chain reaction Shock heating
ISSN:	1355-8145, 1466-1268
Online Access:	Get full text
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