Infection of human lymphomononuclear cells by SARS-CoV-2

Although SARS-CoV-2 severe infection is associated with a hyperinflammatory state, lymphopenia is an immunological hallmark, and correlates with poor prognosis in COVID-19. However, it remains unknown if circulating human lymphocytes and monocytes are susceptible to SARS-CoV-2 infection. In this stu...

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Published in:bioRxiv
Main Authors: Pontelli, Marjorie C, Castro, Italo A, Martins, Ronaldo B, Veras, Flávio P, Serra, Leonardo La, Nascimento, Daniele C, Cardoso, Ricardo S, Rosales, Roberta, Lima, Thais M, Souza, Juliano P, Caetité, Diego B, de Lima, Mikhael H F, Kawahisa, Juliana T, Giannini, Marcela C, Bonjorno, Letícia P, Lopes, Maria I F, Batah, Sabrina S, Siyuan, Li, Assad, Rodrigo L, Almeida, Sergio C L, Oliveira, Fabiola R, Benatti, Maíra N, Pontes, Lorena L F, Santana, Rodrigo C, Vilar, Fernando C, Martins, Maria A, Cunha, Thiago M, Calado, Rodrigo T, Alves-Filho, José C, Zamboni, Dario S, Fabro, Alexandre, Louzada-Junior, Paulo, Oliveira, Rene D R, Cunha, Fernando Q, Arruda, Eurico
Format: Journal Article Paper
Language:English
Published: United States Cold Spring Harbor Laboratory Press 07.08.2020
Cold Spring Harbor Laboratory
Edition:1.2
Subjects:
CD4 antigen
Coronaviruses
COVID-19
Double-stranded RNA
Flow cytometry
Immunofluorescence
Immunohistochemistry
Infections
Leukocytes (mononuclear)
Lymphocytes
Lymphocytes B
Lymphocytes T
Lymphopenia
Microbiology
Monocytes
Peripheral blood mononuclear cells
Severe acute respiratory syndrome coronavirus 2
ISSN:2692-8205, 2692-8205
Online Access:Get full text
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Summary:Although SARS-CoV-2 severe infection is associated with a hyperinflammatory state, lymphopenia is an immunological hallmark, and correlates with poor prognosis in COVID-19. However, it remains unknown if circulating human lymphocytes and monocytes are susceptible to SARS-CoV-2 infection. In this study, SARS-CoV-2 infection of human peripheral blood mononuclear cells (PBMCs) was investigated both and . We found that infection of whole PBMCs from healthy donors was productive of virus progeny. Results revealed that monocytes, as well as B and T lymphocytes, are susceptible to SARS-CoV-2 active infection and viral replication was indicated by detection of double-stranded RNA. Moreover, flow cytometry and immunofluorescence analysis revealed that SARS-CoV-2 was frequently detected in monocytes and B lymphocytes from COVID-19 patients, and less frequently in CD4 T lymphocytes. The rates of SARS-CoV-2-infected monocytes in PBMCs from COVID-19 patients increased over time from symptom onset. Additionally, SARS-CoV-2-positive monocytes and B and CD4+T lymphocytes were detected by immunohistochemistry in post mortem lung tissue. SARS-CoV-2 infection of blood circulating leukocytes in COVID-19 patients may have important implications for disease pathogenesis, immune dysfunction, and virus spread within the host.
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Competing Interest Statement: The authors have declared no competing interest.
ISSN:2692-8205
2692-8205
DOI:10.1101/2020.07.28.225912