Infection of human lymphomononuclear cells by SARS-CoV-2

Although SARS-CoV-2 severe infection is associated with a hyperinflammatory state, lymphopenia is an immunological hallmark, and correlates with poor prognosis in COVID-19. However, it remains unknown if circulating human lymphocytes and monocytes are susceptible to SARS-CoV-2 infection. In this stu...

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Vydané v:	bioRxiv
Hlavní autori:	Pontelli, Marjorie C, Castro, Italo A, Martins, Ronaldo B, Veras, Flávio P, Serra, Leonardo La, Nascimento, Daniele C, Cardoso, Ricardo S, Rosales, Roberta, Lima, Thais M, Souza, Juliano P, Caetité, Diego B, de Lima, Mikhael H F, Kawahisa, Juliana T, Giannini, Marcela C, Bonjorno, Letícia P, Lopes, Maria I F, Batah, Sabrina S, Siyuan, Li, Assad, Rodrigo L, Almeida, Sergio C L, Oliveira, Fabiola R, Benatti, Maíra N, Pontes, Lorena L F, Santana, Rodrigo C, Vilar, Fernando C, Martins, Maria A, Cunha, Thiago M, Calado, Rodrigo T, Alves-Filho, José C, Zamboni, Dario S, Fabro, Alexandre, Louzada-Junior, Paulo, Oliveira, Rene D R, Cunha, Fernando Q, Arruda, Eurico
Médium:	Journal Article Paper
Jazyk:	English
Vydavateľské údaje:	United States Cold Spring Harbor Laboratory Press 07.08.2020 Cold Spring Harbor Laboratory
Vydanie:	1.2
Predmet:	CD4 antigen Coronaviruses COVID-19 Double-stranded RNA Flow cytometry Immunofluorescence Immunohistochemistry Infections Leukocytes (mononuclear) Lymphocytes Lymphocytes B Lymphocytes T Lymphopenia Microbiology Monocytes Peripheral blood mononuclear cells Severe acute respiratory syndrome coronavirus 2
ISSN:	2692-8205, 2692-8205
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Shrnutí:	Although SARS-CoV-2 severe infection is associated with a hyperinflammatory state, lymphopenia is an immunological hallmark, and correlates with poor prognosis in COVID-19. However, it remains unknown if circulating human lymphocytes and monocytes are susceptible to SARS-CoV-2 infection. In this study, SARS-CoV-2 infection of human peripheral blood mononuclear cells (PBMCs) was investigated both and . We found that infection of whole PBMCs from healthy donors was productive of virus progeny. Results revealed that monocytes, as well as B and T lymphocytes, are susceptible to SARS-CoV-2 active infection and viral replication was indicated by detection of double-stranded RNA. Moreover, flow cytometry and immunofluorescence analysis revealed that SARS-CoV-2 was frequently detected in monocytes and B lymphocytes from COVID-19 patients, and less frequently in CD4 T lymphocytes. The rates of SARS-CoV-2-infected monocytes in PBMCs from COVID-19 patients increased over time from symptom onset. Additionally, SARS-CoV-2-positive monocytes and B and CD4+T lymphocytes were detected by immunohistochemistry in post mortem lung tissue. SARS-CoV-2 infection of blood circulating leukocytes in COVID-19 patients may have important implications for disease pathogenesis, immune dysfunction, and virus spread within the host.
Bibliografia:	SourceType-Working Papers-1 ObjectType-Working Paper/Pre-Print-1 content type line 50 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 Competing Interest Statement: The authors have declared no competing interest.
ISSN:	2692-8205 2692-8205
DOI:	10.1101/2020.07.28.225912