The heparin-binding proteome in normal pancreas and murine experimental acute pancreatitis

Acute pancreatitis (AP) is acute inflammation of the pancreas, mainly caused by gallstones and alcohol, driven by changes in communication between cells. Heparin-binding proteins (HBPs) play a central role in health and diseases. Therefore, we used heparin affinity proteomics to identify extracellul...

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Published in:bioRxiv
Main Authors: Nunes, Quentin M, Su, Dunhao, Brownridge, Philip J, Simpson, Deborah M, Sun, Changye, Li, Yong, Phuong Thao P Bui, Zhang, Xiaoying, Huang, Wei, Rigden, Daniel J, Beynon, Robert J, Sutton, Robert, Fernig, David G
Format: Paper
Language:English
Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 29.03.2019
Cold Spring Harbor Laboratory
Edition:1.2
Subjects:
Amyloid
Biochemistry
Biomarkers
Calculi
Complement activation
Glycoproteins
Heparin
Histidine
Histone H2A
Homeostasis
Inflammation
Mitochondria
Pancreas
Pancreatitis
Protein interaction
Proteomics
Acute pancreatitis
heparan sulfate
proteomics
glycosaminoglycan
pancreas
heparin-binding protein
ISSN:2692-8205, 2692-8205
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Abstract Acute pancreatitis (AP) is acute inflammation of the pancreas, mainly caused by gallstones and alcohol, driven by changes in communication between cells. Heparin-binding proteins (HBPs) play a central role in health and diseases. Therefore, we used heparin affinity proteomics to identify extracellular HBPs in pancreas and plasma of normal mice and in a caerulein mouse model of AP. Many new extracellular HBPs (360) were discovered in the pancreas, taking the total number of HBPs known to 786. Extracellular pancreas HBPs form highly interconnected protein-protein interaction networks in both normal pancreas (NP) and AP. Thus, HBPs represent an important set of extracellular proteins with significant regulatory potential in the pancreas. HBPs in NP are associated with biological functions such as molecular transport and cellular movement that underlie pancreatic homeostasis. However, in AP HBPs are associated with additional inflammatory processes such as acute phase response signalling, complement activation and mitochondrial dysfunction, which has a central role in the development of AP. Plasma HBPs in AP included known AP biomarkers such as serum amyloid A, as well as emerging targets such as histone H2A. Other HBPs such as alpha 2-HS glycoprotein (AHSG) and histidine-rich glycoprotein (HRG) need further investigation for potential applications in the management of AP. Pancreas HBPs are extracellular and so easily accessible and are potential drug targets in AP, whereas plasma HBPs represent potential biomarkers for AP. Thus, their identification paves the way to determine which HBPs may have potential applications in the management of AP.
AbstractList Acute pancreatitis (AP) is acute inflammation of the pancreas, mainly caused by gallstones and alcohol, driven by changes in communication between cells. Heparin-binding proteins (HBPs) play a central role in health and diseases. Therefore, we used heparin affinity proteomics to identify extracellular HBPs in pancreas and plasma of normal mice and in a caerulein mouse model of AP. Many new extracellular HBPs (360) were discovered in the pancreas, taking the total number of HBPs known to 786. Extracellular pancreas HBPs form highly interconnected protein-protein interaction networks in both normal pancreas (NP) and AP. Thus, HBPs represent an important set of extracellular proteins with significant regulatory potential in the pancreas. HBPs in NP are associated with biological functions such as molecular transport and cellular movement that underlie pancreatic homeostasis. However, in AP HBPs are associated with additional inflammatory processes such as acute phase response signalling, complement activation and mitochondrial dysfunction, which has a central role in the development of AP. Plasma HBPs in AP included known AP biomarkers such as serum amyloid A, as well as emerging targets such as histone H2A. Other HBPs such as alpha 2-HS glycoprotein (AHSG) and histidine-rich glycoprotein (HRG) need further investigation for potential applications in the management of AP. Pancreas HBPs are extracellular and so easily accessible and are potential drug targets in AP, whereas plasma HBPs represent potential biomarkers for AP. Thus, their identification paves the way to determine which HBPs may have potential applications in the management of AP.
Author Nunes, Quentin M
Fernig, David G
Brownridge, Philip J
Li, Yong
Sutton, Robert
Beynon, Robert J
Su, Dunhao
Simpson, Deborah M
Zhang, Xiaoying
Huang, Wei
Sun, Changye
Phuong Thao P Bui
Rigden, Daniel J
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ContentType Paper
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2019, Posted by Cold Spring Harbor Laboratory
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Keywords Acute pancreatitis
heparan sulfate
proteomics
glycosaminoglycan
pancreas
heparin-binding protein
Language English
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References Gorgani, Smith, Kono, Theofilopoulos (497271v2.38) 2002; 169
Vizcaino, Csordas, del-Toro, Dianes, Griss, Lavidas, Mayer, Perez-Riverol, Reisinger, Ternent, Xu, Wang, Hermjakob (497271v2.64) 2016; 44
Ting, Cowley, Hoon, Guilhaus, Raftery, Cavicchioli (497271v2.21) 2009; 8
Hagiwara, Iwasaka, Shingu, Matsumoto, Uchida, Noguchi (497271v2.56) 2009; 38
Zaret, Grompe (497271v2.1) 2008; 322
Gerasimenko, Gerasimenko, Petersen (497271v2.4) 2014; 592
Pandol, Saluja, Imrie, Banks (497271v2.2) 2007; 132
Schwanhausser, Busse, Li, Dittmar, Schuchhardt, Wolf, Chen, Selbach (497271v2.15) 2013; 495
Gomatos, Xiaodong, Ghaneh, Halloran, Raraty, Lane, Sutton, Neoptolemos (497271v2.17) 2014; 14
Vizcaino, Cote, Csordas, Dianes, Fabregat, Foster, Griss, Alpi, Birim, Contell, O’Kelly, Schoenegger, Ovelleiro, Perez-Riverol, Reisinger, Rios, Wang, Hermjakob (497271v2.35) 2013; 41
Sepulveda-Diaz, Alavi Naini, Huynh, Ouidja, Yanicostas, Chantepie, Villares, Lamari, Jospin, van Kuppevelt, Mensah-Nyagan, Raisman-Vozari, Soussi-Yanicostas, Papy-Garcia (497271v2.44) 2015; 138
Katsumoto, Kume (497271v2.58) 2013; 3
Deutsch, Csordas, Sun, Jarnuczak, Perez-Riverol, Ternent, Campbell, Bernal-Llinares, Okuda, Kawano, Moritz, Carver, Wang, Ishihama, Bandeira, Hermjakob, Vizcaino (497271v2.63) 2017; 45
Haudenschild, Eldridge, Lein, Chromy (497271v2.50) 2014; 455
Xu, Esko (497271v2.9) 2014; 83
De Duve (497271v2.31) 1971; 50
Carvalho, Sousa, Franco, Costa, Neves, Ribeiro, Sutton, Criddle, Soares, de Souza (497271v2.30) 2014; 43
Li, Sun, Yates, Jiang, Wilkinson, Fernig (497271v2.34) 2016
Hartwig, Klafs, Kirschfink, Hackert, Schneider, Gebhard, Buchler, Werner (497271v2.60) 2006; 291
Jambunathan, Galande (497271v2.28) 2014; 8
Ori, Wilkinson, Fernig (497271v2.7) 2011; 286
Duchesne, Octeau, Bearon, Beckett, Prior, Lounis, Fernig (497271v2.51) 2012; 10
Merza, Hartman, Rahman, Hwaiz, Zhang, Renstrom, Luo, Morgelin, Regner, Thorlacius (497271v2.54) 2015; 149
Szklarczyk, Franceschini, Wyder, Forslund, Heller, Huerta-Cepas, Simonovic, Roth, Santos, Tsafou, Kuhn, Bork, Jensen, von Mering (497271v2.26) 2015; 43
Baranzini, Bernard, Oksenberg (497271v2.53) 2005; 174
Nunes, Mournetas, Lane, Sutton, Fernig, Vasieva (497271v2.13) 2013; 13
Fedarko, Conrad (497271v2.43) 1986; 102
Awla, Zetterqvist, Abdulla, Camello, Berglund, Spegel, Pozo, Camello, Regner, Gomez, Thorlacius (497271v2.37) 2012; 143
Kall, Krogh, Sonnhammer (497271v2.23) 2007; 35
Dong, Horvath (497271v2.40) 2007; 1
Magrane (497271v2.25) 2011
Ray (497271v2.32) 1970; 196
Jones, Yazzie, Middaugh (497271v2.48) 2004; 3
Li, Bickel, Biggin (497271v2.16) 2014; 2
Bendtsen, Jensen, Blom, Von Heijne, Brunak (497271v2.24) 2004; 17
Vizcaino, Deutsch, Wang, Csordas, Reisinger, Rios, Dianes, Sun, Farrah, Bandeira, Binz, Xenarios, Eisenacher, Mayer, Gatto, Campos, Chalkley, Kraus, Albar, Martinez-Bartolome (497271v2.36) 2014; 32
Wandschneider, Fehring, Jacobs-Emeis, Thiesen, Lohr (497271v2.19) 2001; 22
Petersen, Brunak, von Heijne, Nielsen (497271v2.22) 2011; 8
Malavaki, Theocharis, Lamari, Kanakis, Tsegenidis, Tzanakakis, Karamanos (497271v2.12) 2011; 25
Balaj, Atai, Chen, Mu, Tannous, Breakefield, Skog, Maguire (497271v2.46) 2015; 5
Schwanhausser, Busse, Li, Dittmar, Schuchhardt, Wolf, Chen, Selbach (497271v2.14) 2011; 473
Vogel, Chothia (497271v2.6) 2006; 2
Coombe, Kett (497271v2.61) 2005; 62
Ishihara, Fedarko, Conrad (497271v2.42) 1986; 261
Silva, Gorenstein, Li, Vissers, Geromanos (497271v2.20) 2006; 5
Xu, Ori, Rudd, Uniewicz, Ahmed, Guimond, Skidmore, Siligardi, Yates, Fernig (497271v2.33) 2012; 287
Poon, Patel, Davis, Parish, Hulett (497271v2.39) 2011; 117
Mayer, Raraty, Slavin, Kemppainen, Fitzpatrick, Hietaranta, Puolakkainen, Beger, Neoptolemos (497271v2.52) 2002; 89
Mukherjee, Mareninova, Odinokova, Huang, Murphy, Chvanov, Javed, Wen, Booth, Cane, Awais, Gavillet, Pruss, Schaller, Molkentin, Tepikin, Petersen, Pandol, Gukovsky, Criddle (497271v2.5) 2015
Chen, Scully, Petralia, Kakkar (497271v2.62) 2014; 15
Christianson, Svensson, van Kuppevelt, Li, Belting (497271v2.45) 2013; 110
Festing (497271v2.18) 2010; 38
Elmore, Coaker (497271v2.29) 2011; 712
Maitre, Heisenberg (497271v2.41) 2013; 23
Wang, Turko (497271v2.47) 2018; 17
Liin, Barro-Soria, Larsson (497271v2.57) 2015; 593
Gong, Meng, Hua, Song, He, Zhou, Qian (497271v2.59) 2014; 9
Liu, Huang, Szatmary, Abrams, Alhamdi, Lin, Greenhalf, Wang, Sutton, Toh (497271v2.55) 2017; 104
Ahmed, Barker, Oliva, Garfin, Talmadge, Georgiou, Quinn, Rice (497271v2.49) 2003; 3
Barzel, Biham (497271v2.27) 2009; 80
Wu, Banks (497271v2.3) 2013; 144
Murphy, Merry, Lyon, Thompson, Roberts, Gallagher (497271v2.11) 2004; 279
Ori, Wilkinson, Fernig (497271v2.8) 2008; 13
Rudd, Yates (497271v2.10) 2012; 8
References_xml – volume: 8
  start-page: 1499
  year: 2012
  end-page: 506
  ident: 497271v2.10
  article-title: A highly efficient tree structure for the biosynthesis of heparan sulfate accounts for the commonly observed disaccharides and suggests a mechanism for domain synthesis
  publication-title: Mol Biosyst
– volume: 132
  start-page: 1127
  year: 2007
  end-page: 51
  ident: 497271v2.2
  article-title: Acute pancreatitis: bench to the bedside
  publication-title: Gastroenterology
– volume: 473
  start-page: 337
  year: 2011
  end-page: 42
  ident: 497271v2.14
  article-title: Global quantification of mammalian gene expression control
  publication-title: Nature
– volume: 144
  start-page: 1272
  year: 2013
  end-page: 81
  ident: 497271v2.3
  article-title: Clinical management of patients with acute pancreatitis
  publication-title: Gastroenterology
– volume: 10
  start-page: e1001361
  year: 2012
  ident: 497271v2.51
  article-title: Transport of fibroblast growth factor 2 in the pericellular matrix is controlled by the spatial distribution of its binding sites in heparan sulfate
  publication-title: PLoS biology
– volume: 89
  start-page: 163
  year: 2002
  end-page: 71
  ident: 497271v2.52
  article-title: Serum amyloid A is a better early predictor of severity than C-reactive protein in acute pancreatitis
  publication-title: Br J Surg
– volume: 110
  start-page: 17380
  year: 2013
  end-page: 5
  ident: 497271v2.45
  article-title: Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for their internalization and functional activity
  publication-title: Proceedings of the National Academy of Sciences of the United States of America
– volume: 38
  start-page: 746
  year: 2009
  end-page: 51
  ident: 497271v2.56
  article-title: Antithrombin III prevents cerulein-induced acute pancreatitis in rats
  publication-title: Pancreas
– volume: 62
  start-page: 410
  year: 2005
  end-page: 24
  ident: 497271v2.61
  article-title: Heparan sulfate-protein interactions: therapeutic potential through structure-function insights
  publication-title: Cellular and molecular life sciences: CMLS
– volume: 169
  start-page: 4745
  year: 2002
  end-page: 51
  ident: 497271v2.38
  article-title: Histidine-rich glycoprotein binds to DNA and Fc gamma RI and potentiates the ingestion of apoptotic cells by macrophages
  publication-title: Journal of immunology
– volume: 117
  start-page: 2093
  year: 2011
  end-page: 101
  ident: 497271v2.39
  article-title: Histidine-rich glycoprotein: the Swiss Army knife of mammalian plasma
  publication-title: Blood
– volume: 41
  start-page: D1063
  year: 2013
  end-page: 9
  ident: 497271v2.35
  article-title: The PRoteomics IDEntifications (PRIDE) database and associated tools: status in 2013
  publication-title: Nucleic acids research
– volume: 279
  start-page: 27239
  year: 2004
  end-page: 45
  ident: 497271v2.11
  article-title: A new model for the domain structure of heparan sulfate based on the novel specificity of K5 lyase
  publication-title: The Journal of biological chemistry
– volume: 25
  start-page: 11
  year: 2011
  end-page: 20
  ident: 497271v2.12
  article-title: Heparan sulfate: biological significance, tools for biochemical analysis and structural characterization
  publication-title: Biomedical chromatography: BMC
– volume: 22
  start-page: 4383
  year: 2001
  end-page: 90
  ident: 497271v2.19
  article-title: Autoimmune pancreatic disease: preparation of pancreatic juice for proteome analysis
  publication-title: Electrophoresis
– start-page: 6
  year: 2016
  ident: 497271v2.34
  article-title: Heparin binding preference and structures in the fibroblast growth factor family parallel their evolutionary diversification
  publication-title: Open Biol
– volume: 291
  start-page: G844
  year: 2006
  end-page: 50
  ident: 497271v2.60
  article-title: Interaction of complement and leukocytes in severe acute pancreatitis: potential for therapeutic intervention
  publication-title: American journal of physiology Gastrointestinal and liver physiology
– volume: 143
  start-page: 1352
  year: 2012
  end-page: 60
  ident: 497271v2.37
  article-title: NFATc3 regulates trypsinogen activation, neutrophil recruitment, and tissue damage in acute pancreatitis in mice
  publication-title: Gastroenterology
– volume: 174
  start-page: 7412
  year: 2005
  end-page: 22
  ident: 497271v2.53
  article-title: Modular transcriptional activity characterizes the initiation and progression of autoimmune encephalomyelitis
  publication-title: Journal of immunology
– volume: 322
  start-page: 1490
  year: 2008
  end-page: 4
  ident: 497271v2.1
  article-title: Generation and regeneration of cells of the liver and pancreas
  publication-title: Science
– volume: 45
  start-page: D1100
  year: 2017
  end-page: D6
  ident: 497271v2.63
  article-title: The ProteomeXchange consortium in 2017: supporting the cultural change in proteomics public data deposition
  publication-title: Nucleic acids research
– start-page: bar009
  year: 2011
  ident: 497271v2.25
  article-title: UniProt Knowledgebase: a hub of integrated protein data
  publication-title: Database: the journal of biological databases and curation
– volume: 149
  start-page: 1920
  year: 2015
  end-page: 31
  ident: 497271v2.54
  article-title: Neutrophil Extracellular Traps Induce Trypsin Activation, Inflammation, and Tissue Damage in Mice With Severe Acute Pancreatitis
  publication-title: Gastroenterology
– volume: 38
  start-page: 681
  year: 2010
  end-page: 90
  ident: 497271v2.18
  article-title: Inbred strains should replace outbred stocks in toxicology, safety testing, and drug development
  publication-title: Toxicologic pathology
– volume: 23
  start-page: R626
  year: 2013
  end-page: 33
  ident: 497271v2.41
  article-title: Three functions of cadherins in cell adhesion
  publication-title: Current biology: CB
– volume: 17
  start-page: 3104
  year: 2018
  end-page: 13
  ident: 497271v2.47
  article-title: Proteomic Toolbox To Standardize the Separation of Extracellular Vesicles and Lipoprotein Particles
  publication-title: J Proteome Res
– volume: 32
  start-page: 223
  year: 2014
  end-page: 6
  ident: 497271v2.36
  article-title: ProteomeXchange provides globally coordinated proteomics data submission and dissemination
  publication-title: Nature biotechnology
– volume: 3
  start-page: 1980
  year: 2003
  end-page: 7
  ident: 497271v2.49
  article-title: An approach to remove albumin for the proteomic analysis of low abundance biomarkers in human serum
  publication-title: Proteomics
– volume: 14
  start-page: 333
  year: 2014
  end-page: 46
  ident: 497271v2.17
  article-title: Prognostic markers in acute pancreatitis
  publication-title: Expert review of molecular diagnostics
– volume: 3
  start-page: 746
  year: 2004
  end-page: 69
  ident: 497271v2.48
  article-title: Polyanions and the proteome
  publication-title: Molecular & Cellular Proteomics
– volume: 8
  start-page: 2227
  year: 2009
  end-page: 42
  ident: 497271v2.21
  article-title: Normalization and statistical analysis of quantitative proteomics data generated by metabolic labeling
  publication-title: Molecular & Cellular Proteomics
– volume: 13
  start-page: 4309
  year: 2008
  end-page: 38
  ident: 497271v2.8
  article-title: The heparanome and regulation of cell function: structures, functions and challenges
  publication-title: Frontiers in Bioscience
– volume: 8
  start-page: 299
  year: 2014
  end-page: 307
  ident: 497271v2.28
  article-title: Sample collection in clinical proteomics--proteolytic activity profile of serum and plasma
  publication-title: Proteomics Clin Appl
– volume: 455
  start-page: 84
  year: 2014
  end-page: 9
  ident: 497271v2.50
  article-title: High abundant protein removal from rodent blood for biomarker discovery
  publication-title: Biochemical and Biophysical Research Communications
– volume: 3
  start-page: 11
  year: 2013
  end-page: 7
  ident: 497271v2.58
  article-title: The role of CXCL12-CXCR4 signaling pathway in pancreatic development
  publication-title: Theranostics
– volume: 5
  start-page: 144
  year: 2006
  end-page: 56
  ident: 497271v2.20
  article-title: Absolute quantification of proteins by LCMSE: a virtue of parallel MS acquisition
  publication-title: Molecular & Cellular Proteomics
– volume: 43
  start-page: D447
  year: 2015
  end-page: 52
  ident: 497271v2.26
  article-title: STRING v10: protein-protein interaction networks, integrated over the tree of life
  publication-title: Nucleic acids research
– volume: 15
  start-page: 135
  year: 2014
  end-page: 45
  ident: 497271v2.62
  article-title: Binding and inhibition of drug transport proteins by heparin: a potential drug transporter modulator capable of reducing multidrug resistance in human cancer cells
  publication-title: Cancer biology & therapy
– volume: 13
  start-page: 598
  year: 2013
  end-page: 604
  ident: 497271v2.13
  article-title: The heparin-binding protein interactome in pancreatic diseases
  publication-title: Pancreatology: official journal of the International Association of Pancreatology
– volume: 261
  start-page: 13575
  year: 1986
  end-page: 80
  ident: 497271v2.42
  article-title: Transport of heparan sulfate into the nuclei of hepatocytes
  publication-title: The Journal of biological chemistry
– volume: 102
  start-page: 587
  year: 1986
  end-page: 99
  ident: 497271v2.43
  article-title: A unique heparan sulfate in the nuclei of hepatocytes: structural changes with the growth state of the cells
  publication-title: The Journal of cell biology
– volume: 1
  start-page: 24
  year: 2007
  ident: 497271v2.40
  article-title: Understanding network concepts in modules
  publication-title: BMC systems biology
– volume: 196
  start-page: 1
  year: 1970
  end-page: 9
  ident: 497271v2.32
  article-title: A modified method for the isolation of the plasma membrane from rat liver
  publication-title: Biochimica et biophysica acta
– volume: 35
  start-page: W429
  year: 2007
  end-page: 32
  ident: 497271v2.23
  article-title: Advantages of combined transmembrane topology and signal peptide prediction--the Phobius web server
  publication-title: Nucleic acids research
– volume: 286
  start-page: 19892
  year: 2011
  end-page: 904
  ident: 497271v2.7
  article-title: A systems biology approach for the investigation of the heparin/heparan sulfate interactome
  publication-title: The Journal of biological chemistry
– volume: 287
  start-page: 40061
  year: 2012
  end-page: 73
  ident: 497271v2.33
  article-title: Diversification of the structural determinants of fibroblast growth factor-heparin interactions: implications for binding specificity
  publication-title: The Journal of biological chemistry
– volume: 104
  start-page: 1215
  year: 2017
  end-page: 25
  ident: 497271v2.55
  article-title: Accuracy of circulating histones in predicting persistent organ failure and mortality in patients with acute pancreatitis
  publication-title: Br J Surg
– volume: 712
  start-page: 31
  year: 2011
  end-page: 44
  ident: 497271v2.29
  article-title: Biochemical purification of native immune protein complexes
  publication-title: Methods Mol Biol
– volume: 2
  start-page: e270
  year: 2014
  ident: 497271v2.16
  article-title: System wide analyses have underestimated protein abundances and the importance of transcription in mammals
  publication-title: PeerJ
– volume: 80
  start-page: 046104
  year: 2009
  ident: 497271v2.27
  article-title: Quantifying the connectivity of a network: the network correlation function method
  publication-title: Physical review E, Statistical, nonlinear, and soft matter physics
– volume: 5
  start-page: 10266
  year: 2015
  ident: 497271v2.46
  article-title: Heparin affinity purification of extracellular vesicles
  publication-title: Sci Rep
– volume: 44
  start-page: D447
  year: 2016
  end-page: 56
  ident: 497271v2.64
  article-title: 2016 update of the PRIDE database and its related tools
  publication-title: Nucleic acids research
– year: 2015
  ident: 497271v2.5
  article-title: Mechanism of mitochondrial permeability transition pore induction and damage in the pancreas: inhibition prevents acute pancreatitis by protecting production of ATP
  publication-title: Gut
– volume: 50
  start-page: 20d
  year: 1971
  end-page: 55d
  ident: 497271v2.31
  article-title: Tissue fractionation. Past and present
  publication-title: The Journal of cell biology
– volume: 8
  start-page: 785
  year: 2011
  end-page: 6
  ident: 497271v2.22
  article-title: SignalP 4.0: discriminating signal peptides from transmembrane regions
  publication-title: Nature methods
– volume: 17
  start-page: 349
  year: 2004
  end-page: 56
  ident: 497271v2.24
  article-title: Feature-based prediction of non-classical and leaderless protein secretion
  publication-title: Protein engineering, design & selection: PEDS
– volume: 43
  start-page: 82
  year: 2014
  end-page: 7
  ident: 497271v2.30
  article-title: Protective effects of fucoidan, a P- and L-selectin inhibitor, in murine acute pancreatitis
  publication-title: Pancreas
– volume: 592
  start-page: 269
  year: 2014
  end-page: 80
  ident: 497271v2.4
  article-title: The role of Ca2+ in the pathophysiology of pancreatitis
  publication-title: The Journal of physiology
– volume: 138
  start-page: 1339
  year: 2015
  end-page: 54
  ident: 497271v2.44
  article-title: HS3ST2 expression is critical for the abnormal phosphorylation of tau in Alzheimer’s disease-related tau pathology
  publication-title: Brain: a journal of neurology
– volume: 495
  start-page: 126
  year: 2013
  end-page: 7
  ident: 497271v2.15
  article-title: Corrigendum: Global quantification of mammalian gene expression control
  publication-title: Nature
– volume: 83
  start-page: 129
  year: 2014
  end-page: 57
  ident: 497271v2.9
  article-title: Demystifying heparan sulfate-protein interactions
  publication-title: Annual review of biochemistry
– volume: 9
  start-page: 1575
  year: 2014
  end-page: 82
  ident: 497271v2.59
  article-title: The SDF-1/CXCR4 axis regulates migration of transplanted bone marrow mesenchymal stem cells towards the pancreas in rats with acute pancreatitis
  publication-title: Molecular medicine reports
– volume: 593
  start-page: 2605
  year: 2015
  end-page: 15
  ident: 497271v2.57
  article-title: The KCNQ1 channel - remarkable flexibility in gating allows for functional versatility
  publication-title: The Journal of physiology
– volume: 2
  start-page: e48
  year: 2006
  ident: 497271v2.6
  article-title: Protein family expansions and biological complexity
  publication-title: PLoS computational biology
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Snippet Acute pancreatitis (AP) is acute inflammation of the pancreas, mainly caused by gallstones and alcohol, driven by changes in communication between cells....
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SubjectTerms Amyloid
Biochemistry
Biomarkers
Calculi
Complement activation
Glycoproteins
Heparin
Histidine
Histone H2A
Homeostasis
Inflammation
Mitochondria
Pancreas
Pancreatitis
Protein interaction
Proteomics
Title The heparin-binding proteome in normal pancreas and murine experimental acute pancreatitis
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https://www.biorxiv.org/content/10.1101/497271
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