Gut microbiome composition predicts summer core range size in a generalist and specialist ungulate

Abstract The gut microbiome of animals varies by age, diet, and habitat, and directly influences individual health. Similarly, variation in an individual’s home range can lead to differences in feeding strategies and fitness. Ungulates (hooved mammals) exhibit species-specific microbiomes and habita...

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Hauptverfasser: Wolf, Jf, Kd Kriss, Macaulay, Km, Munro, K, Patterson, Br, Shafer, Aba
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Veröffentlicht: Cold Spring Harbor Cold Spring Harbor Laboratory Press 26.11.2020
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Abstract Abstract The gut microbiome of animals varies by age, diet, and habitat, and directly influences individual health. Similarly, variation in an individual’s home range can lead to differences in feeding strategies and fitness. Ungulates (hooved mammals) exhibit species-specific microbiomes and habitat use patterns: here, we combined gut microbiome and movement data to assess relationships between space use and the gut microbiome in a specialist and a generalist ungulate. We captured and GPS radiocollared 24 mountain goats (Oreamnos americanus) and 34 white-tailed deer (Odocoileus virginianus). We collected fecal samples and conducted high-throughput sequencing of the 16S rRNA gene. Using GPS data, we estimated core (50%) and home range (95%) sizes and calculated proportional use for several important habitat types. We generated metrics related to gut diversity and key bacterial ratios. We hypothesize that larger Firmicutes to Bacteroides ratios confer body size or fat advantages that allow for larger home ranges, and that relationships between gut diversity and disproportionate habitat use is stronger in mountain goats due to their restricted niche relative to white-tailed deer. Firmicutes to Bacteroides ratios were positively correlated with core range area in both species. Mountain goats exhibited a negative relationship between gut diversity and use of two key habitat types (treed areas and escape terrain), whereas no relationships were detected in white-tailed deer. This is the first study to relate core range size to the gut microbiome in wild ungulates and is an important proof of concept that advances the information that can be gleaned from non-invasive sampling. Competing Interest Statement The authors have declared no competing interest. Footnotes * Revisions and added analyses.
AbstractList The gut microbiome of animals varies by age, diet, and habitat, and directly influences individual health. Similarly, variation in an individual’s home range can lead to differences in feeding strategies and fitness. Ungulates (hooved mammals) exhibit species-specific microbiomes and habitat use patterns: here, we combined gut microbiome and movement data to assess relationships between space use and the gut microbiome in a specialist and a generalist ungulate. We captured and GPS radiocollared 24 mountain goats (Oreamnos americanus) and 34 white-tailed deer (Odocoileus virginianus). We collected fecal samples and conducted high-throughput sequencing of the 16S rRNA gene. Using GPS data, we estimated core (50%) and home range (95%) sizes and calculated proportional use for several important habitat types. We generated metrics related to gut diversity and key bacterial ratios. We hypothesize that larger Firmicutes to Bacteroides ratios confer body size or fat advantages that allow for larger home ranges, and that relationships between gut diversity and disproportionate habitat use is stronger in mountain goats due to their restricted niche relative to white-tailed deer. Firmicutes to Bacteroides ratios were positively correlated with core range area in both species. Mountain goats exhibited a negative relationship between gut diversity and use of two key habitat types (treed areas and escape terrain), whereas no relationships were detected in white-tailed deer. This is the first study to relate core range size to the gut microbiome in wild ungulates and is an important proof of concept that advances the information that can be gleaned from non-invasive sampling.
Abstract The gut microbiome of animals varies by age, diet, and habitat, and directly influences individual health. Similarly, variation in an individual’s home range can lead to differences in feeding strategies and fitness. Ungulates (hooved mammals) exhibit species-specific microbiomes and habitat use patterns: here, we combined gut microbiome and movement data to assess relationships between space use and the gut microbiome in a specialist and a generalist ungulate. We captured and GPS radiocollared 24 mountain goats (Oreamnos americanus) and 34 white-tailed deer (Odocoileus virginianus). We collected fecal samples and conducted high-throughput sequencing of the 16S rRNA gene. Using GPS data, we estimated core (50%) and home range (95%) sizes and calculated proportional use for several important habitat types. We generated metrics related to gut diversity and key bacterial ratios. We hypothesize that larger Firmicutes to Bacteroides ratios confer body size or fat advantages that allow for larger home ranges, and that relationships between gut diversity and disproportionate habitat use is stronger in mountain goats due to their restricted niche relative to white-tailed deer. Firmicutes to Bacteroides ratios were positively correlated with core range area in both species. Mountain goats exhibited a negative relationship between gut diversity and use of two key habitat types (treed areas and escape terrain), whereas no relationships were detected in white-tailed deer. This is the first study to relate core range size to the gut microbiome in wild ungulates and is an important proof of concept that advances the information that can be gleaned from non-invasive sampling. Competing Interest Statement The authors have declared no competing interest. Footnotes * Revisions and added analyses.
Author Patterson, Br
Kd Kriss
Wolf, Jf
Munro, K
Macaulay, Km
Shafer, Aba
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Keywords Mountain goat
Habitat use
GPS tracking
Core range
White-tailed deer
Genomics
Language English
License The copyright holder for this pre-print is the author. All rights reserved. The material may not be redistributed, re-used or adapted without the author's permission.
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Competing Interest Statement: The authors have declared no competing interest.
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Snippet Abstract The gut microbiome of animals varies by age, diet, and habitat, and directly influences individual health. Similarly, variation in an individual’s...
The gut microbiome of animals varies by age, diet, and habitat, and directly influences individual health. Similarly, variation in an individual’s home range...
SourceID biorxiv
proquest
SourceType Open Access Repository
Aggregation Database
SubjectTerms Age
Bacteroides
Body size
Deer
Fecal microflora
Feces
Feeding behavior
Firmicutes
Fitness
Habitat utilization
Habitats
Home range
Intestinal microflora
Microbiomes
Molecular Biology
Next-generation sequencing
Niches
Odocoileus virginianus
Oreamnos
Reproductive behavior
Reproductive status
rRNA 16S
Ungulates
Title Gut microbiome composition predicts summer core range size in a generalist and specialist ungulate
URI https://www.proquest.com/docview/2430528068
https://www.biorxiv.org/content/10.1101/2020.08.04.236638
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