Using eDNA to elucidate Silver and Bighead Carp range expansion in two Missouri River tributaries in eastern South Dakota
This study used environmental DNA (eDNA) to detect Bighead Carp (Hypophthalmichthys nobilis) and Silver Carp (H. molitrix) above and below barriers to fish movement in two tributaries of the Missouri River in eastern South Dakota. The barriers consisted of a natural chain of waterfalls in Sioux Fall...
Gespeichert in:
| Veröffentlicht in: | bioRxiv |
|---|---|
| Hauptverfasser: | , , |
| Format: | Paper |
| Sprache: | Englisch |
| Veröffentlicht: |
Cold Spring Harbor
Cold Spring Harbor Laboratory Press
02.12.2023
Cold Spring Harbor Laboratory |
| Ausgabe: | 1.2 |
| Schlagworte: | |
| ISSN: | 2692-8205, 2692-8205 |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Abstract | This study used environmental DNA (eDNA) to detect Bighead Carp (Hypophthalmichthys nobilis) and Silver Carp (H. molitrix) above and below barriers to fish movement in two tributaries of the Missouri River in eastern South Dakota. The barriers consisted of a natural chain of waterfalls in Sioux Falls in the Big Sioux River and a spillway at the downstream end of Lake Vermillion in the Vermillion River. A total of 213 samples and 38 field blanks were collected over two years (2021/2022). We used filtration methods to collect carp eDNA from the water and qPCR techniques to quantify the amount of eDNA in each sample. The probability of detecting eDNA below the barrier in the Big Sioux River was 51% (95% Credible Interval (CrI): 2 to 97%) and 77% (95% CrI: 8 to 99%) in the Vermillion River. The probability of detecting eDNA above both barriers was significantly smaller: 1% (95% CrI: 0.02 to 24%) for both rivers, with positive above-barrier samples detected only from one sampling date in the Vermillion River. The detection of positive samples above the spillway barrier in the Vermillion River provides the first evidence that Bighead and Silver Carp may have expanded their range to habitats upstream of their documented range in eastern South Dakota. This study demonstrates the utility of using eDNA to detect Bighead and Silver Carp both in areas of known and unknown invasive carp presence in smaller tributary streams to the Missouri River.Competing Interest StatementThe authors have declared no competing interest.Footnotes* updated abstract and title page formatting, added impact, ethics, and COI statements* https://github.com/lindseylabrie/carp_eDNA |
|---|---|
| AbstractList | This study used environmental DNA (eDNA) to detect Bighead Carp (Hypophthalmichthys nobilis) and Silver Carp (H. molitrix) above and below barriers to fish movement in two tributaries of the Missouri River in eastern South Dakota. The barriers consisted of a natural chain of waterfalls in Sioux Falls in the Big Sioux River and a spillway at the downstream end of Lake Vermillion in the Vermillion River. A total of 213 samples and 38 field blanks were collected over two years (2021/2022). We used filtration methods to collect carp eDNA from the water and qPCR techniques to quantify the amount of eDNA in each sample. The probability of detecting eDNA below the barrier in the Big Sioux River was 51% (95% Credible Interval (CrI): 2 to 97%) and 77% (95% CrI: 8 to 99%) in the Vermillion River. The probability of detecting eDNA above both barriers was significantly smaller: 1% (95% CrI: 0.02 to 24%) for both rivers, with positive above-barrier samples detected only from one sampling date in the Vermillion River. The detection of positive samples above the spillway barrier in the Vermillion River provides the first evidence that Bighead and Silver Carp may have expanded their range to habitats upstream of their documented range in eastern South Dakota. This study demonstrates the utility of using eDNA to detect Bighead and Silver Carp both in areas of known and unknown invasive carp presence in smaller tributary streams to the Missouri River.Competing Interest StatementThe authors have declared no competing interest.Footnotes* updated abstract and title page formatting, added impact, ethics, and COI statements* https://github.com/lindseylabrie/carp_eDNA This study used environmental DNA (eDNA) sampling methods to determine the possibility of Bighead Carp Hypophthalmichthys nobilis, and Silver Carp H. molitrix, range expansion above barriers to fish movement in two tributaries of the Missouri River in eastern South Dakota. We collected water samples above and below two perceived barriers to fish movement: a natural chain of waterfalls in the Big Sioux River and a spillway at the downstream end of a manmade reservoir in the Vermillion River. We used filtration methods to collect invasive carp eDNA from water samples and implemented qPCR techniques to quantify the amount of eDNA in each sample. A total of 213 samples and 38 field blanks were collected over two years (2021/2022). We detected carp eDNA below both barriers in both years, and above the barrier in the Vermillion River in 2022. The probability of detecting eDNA below the barrier in the Big Sioux River was 51% (95% Credible Interval (CrI): 2 to 97%) and 77% (95% CrI: 8 to 99%) in the Vermillion River. The probability of detecting eDNA above both barriers was significantly smaller: 1% (95% CrI: 0.02 to 24%) for both rivers. The detection of positive samples above the spillway barrier in the Vermillion River provides the first evidence that Bighead and Silver Carp may have expanded their range to habitats upstream of their documented range in eastern South Dakota. This study demonstrates the utility of using eDNA sampling methods to detect Bighead and Silver Carp in areas of both known and unknown invasive carp presence in smaller tributary streams to the Missouri River. |
| Author | Labrie, Lindsey A P Britten, Hugh B Wesner, Jeff S |
| Author_xml | – sequence: 1 givenname: Lindsey surname: Labrie middlename: A P fullname: Labrie, Lindsey A P – sequence: 2 givenname: Jeff surname: Wesner middlename: S fullname: Wesner, Jeff S – sequence: 3 givenname: Hugh surname: Britten middlename: B fullname: Britten, Hugh B |
| BookMark | eNpNkEtPwkAUhSdGExH5Ae4mceOmOI-20y4RfCWoici6uW0vMIgzdWaK8O8twYWrexbfOTn3XJBTYw0ScsXZkHPGbwUTslNDngyTVAmlTkhPpLmIMsGS03_6nAy8XzPGRJ5yqeIe2c-9NkuKk9cRDZbipq10DQHpTG-26CiYmt7p5QqhpmNwDXVglkhx14Dx2hqqDQ0_lr5o723rNH3XB1twumwDOI3-QCD4gM7QmW3Dik7g0wa4JGcL2Hgc_N0-mT_cf4yfounb4_N4NI1KzmIVxRUrRcqSBBhCVWflokqrOAXFQJQLBrwCKRLkFeZQq5ynCiol8pJnGUdZM9knN8fcUlu309uicfoL3L44rFZwXvCkOK7WoddHtHH2u0UfinX3k-naFSLLOZMilYn8BQFobu4 |
| Cites_doi | 10.18637/jss.v080.i01 10.1080/10618600.1998.10474787 10.3391/ai.2014.9.3.05 10.1007/s001800000038 10.1016/j.jglr.2017.06.003 10.1016/j.scitotenv.2017.06.255 |
| ContentType | Paper |
| Copyright | 2023. This article is published under http://creativecommons.org/licenses/by-nc/4.0/ (“the License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2023, Posted by Cold Spring Harbor Laboratory |
| Copyright_xml | – notice: 2023. This article is published under http://creativecommons.org/licenses/by-nc/4.0/ (“the License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2023, Posted by Cold Spring Harbor Laboratory |
| DBID | 8FE 8FH ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO GNUQQ HCIFZ LK8 M7P PHGZM PHGZT PIMPY PKEHL PQEST PQGLB PQQKQ PQUKI PRINS FX. |
| DOI | 10.1101/2023.11.15.567277 |
| DatabaseName | ProQuest SciTech Collection ProQuest Natural Science Journals ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials Biological Science Collection ProQuest Central Natural Science Collection ProQuest One ProQuest Central ProQuest Central Student SciTech Premium Collection Biological Sciences Biological Science Database ProQuest Central Premium ProQuest One Academic (New) Publicly Available Content Database ProQuest One Academic Middle East (New) ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China bioRxiv |
| DatabaseTitle | Publicly Available Content Database ProQuest Central Student ProQuest One Academic Middle East (New) ProQuest Biological Science Collection ProQuest Central Essentials ProQuest One Academic Eastern Edition ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection Biological Science Database ProQuest SciTech Collection ProQuest Central China ProQuest Central ProQuest One Applied & Life Sciences ProQuest One Academic UKI Edition Natural Science Collection ProQuest Central Korea Biological Science Collection ProQuest Central (New) ProQuest One Academic ProQuest One Academic (New) |
| DatabaseTitleList | Publicly Available Content Database |
| Database_xml | – sequence: 1 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology Ecology |
| EISSN | 2692-8205 |
| Edition | 1.2 |
| ExternalDocumentID | 2023.11.15.567277v2 |
| Genre | Working Paper/Pre-Print |
| GeographicLocations | South Dakota Big Sioux River United States--US Missouri River |
| GeographicLocations_xml | – name: South Dakota – name: Missouri River – name: Big Sioux River – name: United States--US |
| GroupedDBID | 8FE 8FH ABUWG AFKRA ALMA_UNASSIGNED_HOLDINGS AZQEC BBNVY BENPR BHPHI CCPQU DWQXO GNUQQ HCIFZ LK8 M7P NQS PHGZM PHGZT PIMPY PKEHL PQEST PQGLB PQQKQ PQUKI PRINS PROAC RHI FX. |
| ID | FETCH-LOGICAL-b1047-4c0b26055a0eacd8bfc6c46a70a2bf0a1ca325e1ce9ad79167ac729b1881e3d03 |
| IEDL.DBID | M7P |
| ISSN | 2692-8205 |
| IngestDate | Tue Jan 07 18:48:58 EST 2025 Fri Jul 25 09:19:55 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | false |
| IsScholarly | false |
| Language | English |
| License | This pre-print is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), CC BY-NC 4.0, as described at http://creativecommons.org/licenses/by-nc/4.0 |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-b1047-4c0b26055a0eacd8bfc6c46a70a2bf0a1ca325e1ce9ad79167ac729b1881e3d03 |
| Notes | SourceType-Working Papers-1 ObjectType-Working Paper/Pre-Print-1 content type line 50 Competing Interest Statement: The authors have declared no competing interest. |
| ORCID | 0000-0001-6058-7972 0000-0002-9401-925X |
| OpenAccessLink | https://www.proquest.com/docview/2891032635?pq-origsite=%requestingapplication% |
| PQID | 2891032635 |
| PQPubID | 2050091 |
| PageCount | 25 |
| ParticipantIDs | biorxiv_primary_2023_11_15_567277 proquest_journals_2891032635 |
| PublicationCentury | 2000 |
| PublicationDate | 20231202 |
| PublicationDateYYYYMMDD | 2023-12-02 |
| PublicationDate_xml | – month: 12 year: 2023 text: 20231202 day: 02 |
| PublicationDecade | 2020 |
| PublicationPlace | Cold Spring Harbor |
| PublicationPlace_xml | – name: Cold Spring Harbor |
| PublicationTitle | bioRxiv |
| PublicationYear | 2023 |
| Publisher | Cold Spring Harbor Laboratory Press Cold Spring Harbor Laboratory |
| Publisher_xml | – name: Cold Spring Harbor Laboratory Press – name: Cold Spring Harbor Laboratory |
| References | Bagwell, Peters (2023.11.15.567277v2.1) 2019 Thomsen, Kielgast, Iversen, Wiuf, Rasmussen, Gilbert, Orlando, Willerslev (2023.11.15.567277v2.31) 2012; 21 Erickson, Merkes, Jackson, Goforth, Amberg (2023.11.15.567277v2.9) 2017; 43 Kolar, Chapman, Courtenay, Housel, Williams, Jennings (2023.11.15.567277v2.17) 2005 Underhill (2023.11.15.567277v2.32) 1959 (2023.11.15.567277v2.30) 2023 Hayer, Breeggemann, Klumb, Graeb, Bertrand (2023.11.15.567277v2.15) 2014; 9 (2023.11.15.567277v2.25) 2023 Bürkner (2023.11.15.567277v2.5) 2017; 80 Nico, Fuller, Li (2023.11.15.567277v2.19) 2023a Omernik (2023.11.15.567277v2.22) 1987; 77 Song, Small, Casman (2023.11.15.567277v2.29) 2017 Brooks, Gelman (2023.11.15.567277v2.4) 1998; 7 Schmidt, Soluk, Maestas, Britten (2023.11.15.567277v2.28) 2021; 11 Lodge, Turner, Jerde, Barnes, Chadderton, Egan, Feder, Mahon, Pfrender (2023.11.15.567277v2.18) 2012; 21 Nukazawa, Hamasuna, Suzuki (2023.11.15.567277v2.21) 2018; 52 Berkhof, van Mechelen, Hoijtink (2023.11.15.567277v2.2) 2000; 15 Coulter, Wang, Coulter, Van Susteren, Eichmiller, Garvey J, Sorensen (2023.11.15.567277v2.6) 2019; 14 Freedman, Butler, Wahl (2023.11.15.567277v2.14) 2012 Nico, Fuller, Li (2023.11.15.567277v2.20) 2023 (2023.11.15.567277v2.26) 2021 Erickson, Rees, Coulter, Merkes, McCalla, Touzinsky, Walleser, Goforth, Amberg (2023.11.15.567277v2.10) 2016; 16 Flanagan, Mahmood, Umphlett, Haacker, Ray, Sorensen, Shulski, Stiles, Pearson, Fajman (2023.11.15.567277v2.11) 2020; 101 Frankson, Kunkel, Champion, Easterling, Umphlett, Stiles (2023.11.15.567277v2.12) 2022 Dieterman, Berry (2023.11.15.567277v2.7) 1999; 30 Phelps, Tripp, Bales, James, Hrabik, Herzog (2023.11.15.567277v2.24) 2017; 12 Braaten, Berry Jr (2023.11.15.567277v2.3) 1997; 12 Pendleton, Schwinghamer, Solomon, Casper (2023.11.15.567277v2.23) 2017; 100 Rees, Maddison, Middleditch, Patmore, Gough (2023.11.15.567277v2.27) 2014; 51 Jerde, Chadderton, Mahon, Renshaw, Corush, Budny, Mysorekar, Lodge (2023.11.15.567277v2.16) 2013; 70 Eichmiller, Bajer, Sorensen (2023.11.15.567277v2.8) 2014; 9 (2023.11.15.567277v2.33) 2022 Francis, Melliger (2023.11.15.567277v2.13) 2021; 18 |
| References_xml | – volume: 16 start-page: 957 issue: 4 year: 2016 end-page: 965 ident: 2023.11.15.567277v2.10 article-title: Detecting the movement and spawning activity of bigheaded carps with environmental DNA publication-title: Mol. Ecol. Resour – volume: 80 year: 2017 ident: 2023.11.15.567277v2.5 article-title: . brms : An R Package for Bayesian Multilevel Models Using Stan publication-title: J. Stat. Softw doi: 10.18637/jss.v080.i01 – year: 2012 ident: 2023.11.15.567277v2.14 publication-title: Final project report to Illinois–Indiana Sea Grant – volume: 7 start-page: 434 year: 1998 end-page: 455 ident: 2023.11.15.567277v2.4 article-title: General Methods for Monitoring Convergence of Iterative Simulations publication-title: J. Comput. Graphi. Stat doi: 10.1080/10618600.1998.10474787 – year: 2023 ident: 2023.11.15.567277v2.25 article-title: DNeasy Blood & Tissue Handbook – volume: 14 start-page: e0218823 issue: 6 year: 2019 ident: 2023.11.15.567277v2.6 article-title: Nonlinear relationship between Silver Carp density and their eDNA concentration in a large river publication-title: PLoS One – volume: 51 start-page: 1450 issue: 5 year: 2014 end-page: 1459 ident: 2023.11.15.567277v2.27 article-title: The detection of aquatic animal species using environmental DNA–a review of eDNA as a survey tool in ecology publication-title: J. Appl. Ecol – volume: 9 start-page: e112611 issue: 11 year: 2014 ident: 2023.11.15.567277v2.8 article-title: The relationship between the distribution of common carp and their environmental DNA in a small lake publication-title: PLoS One – year: 2022 ident: 2023.11.15.567277v2.33 article-title: Quality Assurance Project Plan (QAPP) eDNA monitoring of bighead and silver carps publication-title: U.S. Fish and Wildlife Service, Midwest Region – year: 2023a ident: 2023.11.15.567277v2.19 article-title: Hypophthalmichthys molitrix (Valenciennes in Cuvier and Valenciennes, 1844) publication-title: Nonindigenous Aquatic Species Database – year: 2005 ident: 2023.11.15.567277v2.17 article-title: Asian Carps of the Genus Hypophthalmichthys (Pisces publication-title: Cyprinidae) ― A Biological Synopsis and Environmental Risk Assessment – volume: 101 start-page: E817 issue: 6 year: 2020 end-page: E829 ident: 2023.11.15.567277v2.11 article-title: A hydrometeorological assessment of the historic 2019 flood of Nebraska, Iowa, and South Dakota publication-title: Bull Am Meteorol Soc – volume: 9 start-page: 289 year: 2014 end-page: 303 ident: 2023.11.15.567277v2.15 article-title: Population characteristics of bighead and silver carp on the northwestern front of their North American invasion publication-title: Aquat. Invasions doi: 10.3391/ai.2014.9.3.05 – volume: 12 start-page: 477 issue: 3 year: 1997 end-page: 489 ident: 2023.11.15.567277v2.3 article-title: Fish associations with four habitat types in a South Dakota prairie stream publication-title: J. Freshw. Ecol – volume: 77 start-page: 118 issue: 1 year: 1987 end-page: 125 ident: 2023.11.15.567277v2.22 article-title: Ecoregions of the conterminous United States publication-title: Ann Assoc Am Geogr – volume: 52 start-page: 10562 issue: 18 year: 2018 end-page: 10570 ident: 2023.11.15.567277v2.21 article-title: Simulating the advection and degradation of the environmental DNA of common carp along a river publication-title: Environ. Sci. Technol – year: 2019 ident: 2023.11.15.567277v2.1 article-title: Analysis of the 2019 Midwest US Flooding using NASA data publication-title: Paper presented at the AGU Fall Meeting Abstracts – volume: 30 start-page: 199 year: 1999 end-page: 224 ident: 2023.11.15.567277v2.7 article-title: Fish community and water quality changes in the Big Sioux River publication-title: Prairie Naturalist – year: 2023 ident: 2023.11.15.567277v2.20 article-title: Hypophthalmichthys nobilis (Richardson, 1845). Nonindigenous Aquatic Species Database – start-page: 5 year: 2022 ident: 2023.11.15.567277v2.12 publication-title: NOAA/NESDIS – volume: 11 start-page: 18987 issue: 1 year: 2021 ident: 2023.11.15.567277v2.28 article-title: Persistence and accumulation of environmental DNA from an endangered dragonfly publication-title: Sci. Rep – year: 2023 ident: 2023.11.15.567277v2.30 publication-title: RStan: the R interface to Stan – year: 1959 ident: 2023.11.15.567277v2.32 article-title: Fishes of the Vermillion River publication-title: South Dakota. Paper presented at the Proc SD Acad Sci – volume: 12 start-page: e0184081 issue: 9 year: 2017 ident: 2023.11.15.567277v2.24 article-title: Incorporating basic and applied approaches to evaluate the effects of invasive Asian Carp on native fishes: A necessary first step for integrated pest management publication-title: PLoS One – volume: 15 start-page: 337 issue: 3 year: 2000 end-page: 354 ident: 2023.11.15.567277v2.2 article-title: Posterior predictive checks: Principles and discussion publication-title: Comput. Stat doi: 10.1007/s001800000038 – volume: 100 start-page: 1213 year: 2017 end-page: 1222 ident: 2023.11.15.567277v2.23 article-title: Competition among river planktivores: are native planktivores still fewer and skinnier in response to the Silver Carp invasion? publication-title: Environ. Biol. Fishes – volume: 70 start-page: 522 issue: 4 year: 2013 end-page: 526 ident: 2023.11.15.567277v2.16 article-title: Detection of Asian carp DNA as part of a Great Lakes basin-wide surveillance program publication-title: Can. J. Fish. Aquat. Sci – volume: 43 start-page: 762 issue: 4 year: 2017 end-page: 770 ident: 2023.11.15.567277v2.9 article-title: Seasonal trends in eDNA detection and occupancy of bigheaded carps publication-title: J. Great Lakes Res doi: 10.1016/j.jglr.2017.06.003 – volume: 21 start-page: 2555 issue: 11 year: 2012 end-page: 2558 ident: 2023.11.15.567277v2.18 article-title: Conservation in a cup of water: estimating biodiversity and population abundance from environmental DNA publication-title: Mol. ecol – volume: 21 start-page: 2565 issue: 11 year: 2012 end-page: 2573 ident: 2023.11.15.567277v2.31 article-title: Monitoring endangered freshwater biodiversity using environmental DNA publication-title: Mol. ecol – year: 2021 ident: 2023.11.15.567277v2.26 publication-title: R: A Language and Environment for Statistical Computing – start-page: 605 year: 2017 end-page: 606 ident: 2023.11.15.567277v2.29 article-title: Making sense of the noise: The effect of hydrology on silver carp eDNA detection in the Chicago area waterway system publication-title: Sci. Total Environ doi: 10.1016/j.scitotenv.2017.06.255 – volume: 18 start-page: 20 issue: 1 year: 2021 end-page: 28 ident: 2023.11.15.567277v2.13 article-title: 2019 Missouri River Basin Historic Flood Event publication-title: Journal of Dam Safety |
| SSID | ssj0002961374 |
| Score | 1.7069145 |
| SecondaryResourceType | preprint |
| Snippet | This study used environmental DNA (eDNA) to detect Bighead Carp (Hypophthalmichthys nobilis) and Silver Carp (H. molitrix) above and below barriers to fish... This study used environmental DNA (eDNA) sampling methods to determine the possibility of Bighead Carp Hypophthalmichthys nobilis, and Silver Carp H. molitrix,... |
| SourceID | biorxiv proquest |
| SourceType | Open Access Repository Aggregation Database |
| SubjectTerms | Ecology Environmental DNA Range extension Rivers |
| Title | Using eDNA to elucidate Silver and Bighead Carp range expansion in two Missouri River tributaries in eastern South Dakota |
| URI | https://www.proquest.com/docview/2891032635 https://www.biorxiv.org/content/10.1101/2023.11.15.567277 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3JatxAEG3iGRt8cuLYxCsVyFWO1BptJ-MVB-JBeMM5iepFIByksUZe5u9d1SMnB4MvvklICNGlft1V9fSeED90bAxiYLxkVFqPUJKmVJSVnlEEjcZiKF3H9OZ3Mh6nt7dZ3hfcpj2t8hUTHVCbRnON_CclBqz9Ruvj_uTeY9co7q72FhoLYsgqCaGj7uX_aiwyo8XKCTHLOKOJL_2ob2zSh8hpf0hHe0G0F3E_kmWfVNW0z9XjG2B2q83pykff87MY5jix7RfxydarYmnuNzn7KmaOIAD2eHwAXQP274OuOOOHy4oJ0oC1gUMmfqCBI2wn0PKvB2CfCTK4qgZVDd1TA-cULS7ewwWzOmBumuWSbr6D3YBsW4Nz54NjvGs6XBPXpydXR2de773gKSfeMNK-4lQnQp-g2aSq1LEexZj4KFXpY6ApjJENtM3QJLTHTFDTPl0FaRrY0PjhuhjUTW2_CShHJpKWtjHIWnt-gNrGmoXEpEoVynJDfO-HvZjMFTYKDg3lJkUQFfPQbIjt19Eu-kk2Lf4P9eb7l7fEMj_RsVDkthh07YPdEYv6saum7a4YHp6M84td9-3QWf7rPP_zAkcezGo |
| linkProvider | ProQuest |
| linkToHtml | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9QwEB6VLghOvEWhBSPBMSVx3geE2m6rVt1Gq1JQb-nEdqSIKtlm08f-KX4jM062PSBx64FbpERWkvn8zdMzAJ9UpDWip504KI1DLElbKkxLRxdEjdqgL23G9OckzrLk9DSdrsDv5VkYLqtccqIlat0ojpF_IceAe7-Rfvw2u3B4ahRnV5cjNHpYHJrFNbls868HY5LvZyn3dk929p1hqoBT2LYEgXILNuJDdIl0dFKUKlJBhLGLsihd9BS9YGg8ZVLUMVlPMSqyQAsvSTzja9endR_AKPCDmPbVaHs3mx7fRnVkSurRtn6WUUpUI91wSKUS9DnQ4NPVphduhpwB5UZTRdW0N9XVX6rA6re9p__bn3kGoynOTPscVkz9Ah71EzUXL2FhSyCEGWdbomuEOb9UFcc0xPeKS8AF1lpsc2kLarGD7Uy0fLhCmBsiRY4biqoW3XUjjgiPnJ4Qx1y3IvqxYDaswE_wvCPT1sLOHxRj_NV0-Ap-3MvXvobVuqnNGxBloENpyFBD7iboeqhMpLhVmiySAmW5Bh8HMeezvodIzlAg7yv3wryHwhqsL6WbDzQyz-9E-_bftz_A4_2To0k-OcgO38ETXt3W3Mh1WO3aS7MBD9VVV83b9wNiBZzdNxT-AH23KCE |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Using+eDNA+to+elucidate+Silver+and+Bighead+Carp+range+expansion+in+two+Missouri+River+tributaries+in+eastern+South+Dakota&rft.jtitle=bioRxiv&rft.au=Labrie%2C+Lindsey+A+P&rft.au=Wesner%2C+Jeff+S&rft.au=Britten%2C+Hugh+B&rft.date=2023-12-02&rft.pub=Cold+Spring+Harbor+Laboratory+Press&rft.issn=2692-8205&rft.eissn=2692-8205&rft_id=info:doi/10.1101%2F2023.11.15.567277 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2692-8205&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2692-8205&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2692-8205&client=summon |