Essential Techniques for Medical and Life Scientists

This handbook covers some primary instruments-based techniques used in modern biological science and medical research programs. Key features of the book include introductory notes for each topic, a systematic presentation of relevant methods, and troubleshooting guides for practical settings. Topics...

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Bibliographic Details
Main Author: Tutar, Yusuf
Format: eBook
Language:English
Published: Singapore Bentham Science Publishers 2020
Edition:1
Subjects:
Life sciences
Medical sciences-Research
Spectrum analysis
ISBN:9811464847, 9789811464843
Online Access:Get full text
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Table of Contents:
  • Transmission Electron Microscopy: Theory and Applications -- Charu Arora*, Sanju Soni and Pathik Maji -- 1. INTRODUCTION -- 2. THEORY -- 3. INSTRUMENTATION -- 4. PREPARATION OF SAMPLES AND WORKING APPLICATIONS -- 5. CHALLENGES WITH BIOLOGICAL ELECTRON MICROSCOPY -- 5.1. Vacuum -- 5.2. Lack of Contrast -- 5.3. Transparency -- 5.4. Charging -- 6. DEVELOPMENT OF SCANNING TRANSMISSION ELECTRON MICROSCOPE -- CONCLUSION -- CONSENT FOR PUBLICATION -- CONFLICT OF INTEREST -- ACKNOWLEDGEMENTS -- REFERENCES -- Scanning Electron Microscopy: Theory and Applications -- Charu Arora*, Sumantra Bhattacharya, Sanju Soni and Pathik Maji -- 1. INTRODUCTION -- 2. INSTRUMENTATION -- 3. SAMPLE PREPARATION -- 4. APPLICATIONS OF SCANNING ELECTRON MICROSCOPY (SEM) -- CONSENT FOR PUBLICATION -- CONFLICT OF INTEREST -- ACKNOWLEDGEMENTS -- REFERENCES -- SEM-EDX: A Potential Tool for Studies of Medicinal Plants -- Iqbal Ansari1, Muniyan Sundararajan1, Ritesh Kumar1, Sadanand Sharma1 and Charu Arora2,* -- 1. INTRODUCTION -- 2. DETAILS OF SEM-EDS -- 3. PRINCIPLE, WORKING AND PROCEDURE -- 4. LITERATURE REVIEW WITH EMPHASIS ON SEM-EDX STUDY OF MEDICINAL PLANTS -- 5. MEDICINAL PLANTS IN JHARKHAND STATE: AN OVERVIEW -- 6. SIGNIFICANCE -- CONCLUSION -- CONSENT FOR PUBLICATION -- CONFLICT OF INTEREST -- ACKNOWLEDGEMENTS -- REFERENCES -- Subject Index -- Back Cover
  • Cover -- Title -- Copyright -- End User License Agreement -- Contents -- Foreword -- Preface -- List of Contributors -- Fourier-Transform Mid-Infrared (FT-MIR) Spectroscopy in Biomedicine -- Bernardo Ribeiro da Cunha1,2, Luís Ramalhete1,3, Luís P. Fonseca2 and Cecília R.C. Calado1,* -- 1. INTRODUCTION -- 2. DISPERSIVE VERSUS INTERFEROGRAM BASED EQUIPMENT -- 3. TYPES OF SAMPLES, DETECTION MODES AND DATA ANALYSIS METHODS -- 4. FT-MIR SPECTROSCOPY AND SYSTEMS BIOLOGY -- 5. NIR SPECTROSCOPY (NIRS) -- 6. RAMAN SPECTROSCOPY -- 7. DATA ACQUISITION MODES, PRE-PROCESSING AND PROCESSING METHODS -- 7.1. Spectra Acquisition Modes -- 7.2. Quality Tests And Spectral Pre-processing -- 7.3. Biochemical And Metabolic Interpretation -- 7.4. Dimensionality Reduction -- 7.5. Pattern Recognition And Multivariate Regression -- 7.6. Advanced Algorithms -- 8. APPLICATIONS IN CLINICAL DIAGNOSIS -- 8.1. Cancer -- 8.2. Brain Disorders -- FINAL REMARKS -- CONSENT FOR PUBLICATION -- CONFLICT OF INTEREST -- ACKNOWLEDGEMENTS -- REFERENCES -- High Performance Liquid Chromatography (HPLC) - Theoretical and Practical Aspects -- Esen Tutar* -- 1. INTRODUCTION -- 2. HPLC INSTRUMENT -- 2.1. Solvent Reservoir (Mobile Phase) -- 2.2. Pump (Solvent Delivery System) -- 2.2.1. Pump Types -- 2.2.2. Isocratic And Gradient Elution Mode -- 2.3. Sample Injection System -- 2.4. The Column (The stationary phase) -- 2.4.1. The Column Types -- 2.4.2. The Column Care, Maintenance And Storage -- 2.5. Detectors -- 2.5.1. Detector Characteristics -- 2.5.2. Detector Types -- 3. HPLC TYPES -- 4. HPLC PROTOCOLS -- 4.1. Sample Preparation -- 4.2. Quantitative Analysis -- 5. HPLC APPLICATIONS -- CONSENT FOR PUBLICATION -- CONFLICT OF INTEREST -- ACKNOWLEDGEMENTS -- REFERENCES -- Raman Spectroscopy and Its Biomedical Applications -- Charu Arora1,*, Pathik Maji1 and P. K. Bajpai2 -- 1. INTRODUCTION
  • 2. THEORETICAL BACKGROUND -- 2.1. Raman Scattering -- 2.2. Instrumentation for Raman Spectroscopy -- 2.3. Instrument Set Up -- 2.3.1. Source -- 2.3.2. Sample Handing Techniques -- 2.3.3. Filter -- 2.3.4. Monochromator -- 2.3.5. Detector -- 2.4. Scanning of Raman Spectra -- 3. TECHNIQUES OF RAMAN SPECTROSCOPY -- 3.1. Confocal Raman Spectroscopy -- 3.2. Raman Micro Spectroscopy -- 3.3. Selective-sampling Raman micro-spectroscopy -- 3.4. Surface Enhanced Raman Spectroscopy -- 3.5. Spatially Offset Raman Spectroscopy and Transmission Raman Spectroscopy -- 3.6. Fiber-Optic Raman Probes -- 3.7. Coherent Anti-Stokes Raman Spectroscopy and Stimulated Raman Spectroscopy -- 4. BIOMEDICAL APPLICATIONS -- 4.1. Raman Based Study Of Human Skin -- 4.2. Measurement Condition -- 4.3. In Vivo Cancer Detection by Raman Spectroscopy -- 4.4. Biofluid Diagnostic Assays Based on Raman Spectroscopy -- 4.5. Raman Analysis Of Sperm Nuclear DNA Integrity -- 4.6. Raman Investigation Of Microorganisms On Single Cell Level -- 4.7. Investigating the Atherosclerosis Process -- 4.8. Thrombosis Mechanisms Using High Resolution Surface Enhanced Raman Spectroscopy -- 4.9. Structure Determination Of Gelonin As A Biomolecule By Raman Spectroscopy -- 4.9.1. Classification -- 4.9.2. Measurement -- 4.9.3. Assignment of Proteins -- CONCLUSION -- CONSENT FOR PUBLICATION -- CONFLICT OF INTEREST -- ACKNOWLEDGEMENTS -- REFERENCES -- Circular Dichroism -- Yusuf Tutar1,2,* -- 1. INTRODUCTION -- 2. INSTRUMENTATION AND SAMPLE PREPARATION -- 3. DATA COLLECTION AND PROCESSING -- 4. APPLICATIONS -- 4.1. Secondary Structure Content Of Proteins -- 4.1.1. Detecting Conformational Alterations Upon Ligand Binding -- 4.2. Protein Stability -- 5. PROTOCOL FOR CD MEASUREMENT -- 6. SUMMARY -- CONSENT FOR PUBLICATION -- CONFLICT OF INTEREST -- ACKNOWLEDGEMENTS -- REFERENCES