Circular Whole-Transcriptome Amplification (cWTA) and mNGS Screening Enhanced by a Group Testing Algorithm (mEGA) Enable High-Throughput and Comprehensive Virus Identification

Given the breadth of pathogens that cause infections, a single approach that can detect a wide range of pathogens is ideal but is impractical due to the available tests being highly specific to a certain pathogen. Recent developments in sequencing technology have introduced mNGS as an alternative th...

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Veröffentlicht in:	mSphere Jg. 7; H. 5; S. e0033222
Hauptverfasser:	Reteng, Patrick, Nguyen Thuy, Linh, Rahman, Mizanur, Bispo de Filippis, Ana Maria, Hayashida, Kyoko, Sugi, Tatsuki, Gonzalez, Gabriel, Hall, William W., Nguyen Thi, Lan Anh, Yamagishi, Junya
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States American Society for Microbiology 26.10.2022
Schlagworte:	Algorithms Chikungunya virus Clinical Microbiology comprehensive pathogen detection Dengue fever Diagnosis Feasibility studies febrile illness Genomes group testing algorithm Hepatitis B High-Throughput Nucleotide Sequencing High-Throughput Nucleotide Sequencing - methods High-throughput screening Humans Infectious diseases metagenomic Metagenomics Microbiology multiple displacement amplification Next-generation sequencing Nucleic Acids Parvoviruses Pathogens QR1-502 Research Article RNA Transcriptome Transcriptomes Viruses Bangladesh metagenomic group testing algorithm comprehensive pathogen detection multiple displacement amplification febrile illness
ISSN:	2379-5042, 2379-5042
Online-Zugang:	Volltext
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