MALDI-FTICR imaging mass spectrometry of drugs and metabolites in tissue

A new approach is described for imaging mass spectrometry analysis of drugs and metabolites in tissue using matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FTICR). The technique utilizes the high resolving power to produce images from thousands of ions me...

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Bibliographic Details
Published in:Analytical chemistry (Washington) Vol. 80; no. 14; p. 5648
Main Authors: Cornett, Dale S, Frappier, Sara L, Caprioli, Richard M
Format: Journal Article
Language:English
Published: United States 15.07.2008
Subjects:
Animals
Fluorescence Resonance Energy Transfer - methods
Liver - metabolism
Male
Mice
Molecular Structure
Pharmaceutical Preparations - analysis
Pharmaceutical Preparations - chemistry
Pharmaceutical Preparations - metabolism
Rats
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
ISSN:1520-6882, 1520-6882
Online Access:Get more information
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Summary:A new approach is described for imaging mass spectrometry analysis of drugs and metabolites in tissue using matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FTICR). The technique utilizes the high resolving power to produce images from thousands of ions measured during a single mass spectrometry (MS)-mode experiment. Accurate mass measurement provides molecular specificity for the ion images on the basis of elemental composition. Final structural confirmation of the targeted compound is made from accurate mass fragment ions generated in an external quadrupole-collision cell. The ability to image many small molecules in a single measurement with high specificity is a significant improvement over existing MS/MS based technologies. Example images are shown for olanzapine in kidney and liver and imatinib in glioma.
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ISSN:1520-6882
1520-6882
DOI:10.1021/ac800617s