Primer, Pipelines, Parameters: Issues in 16S rRNA Gene Sequencing

In 16S rRNA gene sequencing, certain bacterial genera were found to be underrepresented or even missing in taxonomic profiles when using unsuitable primer combinations, outdated reference databases, or inadequate pipeline settings. Concerning the last, quality thresholds as well as bioinformatic set...

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Bibliographic Details

Published in:	mSphere Vol. 6; no. 1
Main Authors:	Abellan-Schneyder, Isabel, Matchado, Monica S., Reitmeier, Sandra, Sommer, Alina, Sewald, Zeno, Baumbach, Jan, List, Markus, Neuhaus, Klaus
Format:	Journal Article
Language:	English
Published:	United States American Society for Microbiology 24.02.2021
Subjects:	Research Article databases variable regions bioinformatic settings mock communities 16S rRNA gene sequencing amplicon sequencing clustering microbiome
ISSN:	2379-5042, 2379-5042
Online Access:	Get full text
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