Use of urine volatile organic compounds to discriminate tuberculosis patients from healthy subjects

Development of noninvasive methods for tuberculosis (TB) diagnosis, with the potential to be administered in field situations, remains as an unmet challenge. A wide array of molecules are present in urine and reflect the pathophysiological condition of a subject. With infection, an alteration in the...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) Vol. 83; no. 14; p. 5526
Main Authors: Banday, Khalid Muzaffar, Pasikanti, Kishore Kumar, Chan, Eric Chun Yong, Singla, Rupak, Rao, Kanury Venkata Subba, Chauhan, Virander Singh, Nanda, Ranjan Kumar
Format: Journal Article
Language:English
Published: United States 15.07.2011
Subjects:
Adult
Aged
Female
Gas Chromatography-Mass Spectrometry - methods
Humans
Male
Middle Aged
Multivariate Analysis
Reproducibility of Results
Tuberculosis - diagnosis
Tuberculosis - urine
Volatile Organic Compounds - urine
Young Adult
ISSN:1520-6882, 1520-6882
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Development of noninvasive methods for tuberculosis (TB) diagnosis, with the potential to be administered in field situations, remains as an unmet challenge. A wide array of molecules are present in urine and reflect the pathophysiological condition of a subject. With infection, an alteration in the molecular constituents is anticipated, characterization of which may form a basis for TB diagnosis. In the present study volatile organic compounds (VOCs) in human urine derived from TB patients and healthy controls were identified and quantified using headspace gas chromatography/mass spectrometry (GC/MS). We found significant (p < 0.05) increase in the abundance of o-xylene (6.37) and isopropyl acetate (2.07) and decreased level of 3-pentanol (0.59), dimethylstyrene (0.37), and cymol (0.42) in TB patients compared to controls. These markers could discriminate TB from healthy controls and related diseases like lung cancer and chronic obstructive pulmonary disorder. This study suggests a possibility of using urinary VOCs for the diagnosis of human TB.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Undefined-1
ObjectType-Feature-3
content type line 23
ISSN:1520-6882
1520-6882
DOI:10.1021/ac200265g