The Molecular Tweezer CLR01 Stabilizes a Disordered Protein-Protein Interface

Protein regions that are involved in protein-protein interactions (PPIs) very often display a high degree of intrinsic disorder, which is reduced during the recognition process. A prime example is binding of the rigid 14-3-3 adapter proteins to their numerous partner proteins, whose recognition moti...

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Veröffentlicht in:Journal of the American Chemical Society Jg. 139; H. 45; S. 16256
Hauptverfasser: Bier, David, Mittal, Sumit, Bravo-Rodriguez, Kenny, Sowislok, Andrea, Guillory, Xavier, Briels, Jeroen, Heid, Christian, Bartel, Maria, Wettig, Burkhard, Brunsveld, Luc, Sanchez-Garcia, Elsa, Schrader, Thomas, Ottmann, Christian
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 15.11.2017
Schlagworte:
14-3-3 Proteins - chemistry
14-3-3 Proteins - metabolism
Amino Acid Motifs
Binding Sites
cdc25 Phosphatases - chemistry
cdc25 Phosphatases - metabolism
Entropy
Intrinsically Disordered Proteins - chemistry
Intrinsically Disordered Proteins - metabolism
Ligands
Models, Molecular
Peptides - chemistry
Peptides - metabolism
Protein Binding
Protein Conformation
Protein Stability
ISSN:1520-5126, 1520-5126
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Zusammenfassung:Protein regions that are involved in protein-protein interactions (PPIs) very often display a high degree of intrinsic disorder, which is reduced during the recognition process. A prime example is binding of the rigid 14-3-3 adapter proteins to their numerous partner proteins, whose recognition motifs undergo an extensive disorder-to-order transition. In this context, it is highly desirable to control this entropy-costly process using tailored stabilizing agents. This study reveals how the molecular tweezer CLR01 tunes the 14-3-3/Cdc25CpS216 protein-protein interaction. Protein crystallography, biophysical affinity determination and biomolecular simulations unanimously deliver a remarkable finding: a supramolecular "Janus" ligand can bind simultaneously to a flexible peptidic PPI recognition motif and to a well-structured adapter protein. This binding fills a gap in the protein-protein interface, "freezes" one of the conformational states of the intrinsically disordered Cdc25C protein partner and enhances the apparent affinity of the interaction. This is the first structural and functional proof of a supramolecular ligand targeting a PPI interface and stabilizing the binding of an intrinsically disordered recognition motif to a rigid partner protein.
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ISSN:1520-5126
1520-5126
DOI:10.1021/jacs.7b07939