Trapping of the Enoyl-Acyl Carrier Protein Reductase-Acyl Carrier Protein Interaction

An ideal target for metabolic engineering, fatty acid biosynthesis remains poorly understood on a molecular level. These carrier protein-dependent pathways require fundamental protein Protein interactions to guide reactivity and processivity, and their control has become one of the major hurdles in...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Journal of the American Chemical Society Ročník 138; číslo 12; s. 3962 - 3965
Hlavní autoři: Tallorin, Lorillee, Finzel, Kara, Nguyen, Quynh G., Beld, Joris, La Clair, James J., Burkart, Michael D.
Médium: Journal Article
Jazyk:angličtina
Vydáno: WASHINGTON Amer Chemical Soc 30.03.2016
Témata:
Acyl Carrier Protein - metabolism
Chemistry
Chemistry, Multidisciplinary
Enoyl-(Acyl-Carrier-Protein) Reductase (NADH) - isolation & purification
Enoyl-(Acyl-Carrier-Protein) Reductase (NADH) - metabolism
Escherichia coli - enzymology
Escherichia coli Proteins - isolation & purification
Escherichia coli Proteins - metabolism
Fatty Acid Synthase, Type II - isolation & purification
Fatty Acid Synthase, Type II - metabolism
Models, Molecular
Molecular Structure
Oxidoreductases - isolation & purification
Oxidoreductases - metabolism
Physical Sciences
Protein Binding
Protein Engineering
Science & Technology
Triclosan - chemistry
Triclosan - metabolism
TARGET
TRICLOSAN
FABI
INHIBITION
FATTY-ACID BIOSYNTHESIS
MECHANISM
ANALOGS
PLASMODIUM-FALCIPARUM
ESCHERICHIA-COLI
BINDING
ISSN:0002-7863, 1520-5126
On-line přístup:Zjistit podrobnosti o přístupu
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Abstract An ideal target for metabolic engineering, fatty acid biosynthesis remains poorly understood on a molecular level. These carrier protein-dependent pathways require fundamental protein Protein interactions to guide reactivity and processivity, and their control has become one of the major hurdles in successfully adapting these biological machines. Our laboratory has developed methods to prepare acyl carrier proteins (ACPs) loaded with substrate mimetics and cross-linkers to visualize and trap interactions with partner enzymes, and we continue to expand the tools for studying these pathways. We now describe application of the slow-onset, tight-binding inhibitor triclosan to explore the interactions between the type II fatty acid ACP from Escherichia coli, AcpP,, and its corresponding enoyl-ACP Teductase, FabI. We show that the AcpP-triclosan complex demonstrates nM binding, inhibits in vitro activity, and can be used to isolate FabI in complex proteomes.
AbstractList An ideal target for metabolic engineering, fatty acid biosynthesis remains poorly understood on a molecular level. These carrier protein-dependent pathways require fundamental protein Protein interactions to guide reactivity and processivity, and their control has become one of the major hurdles in successfully adapting these biological machines. Our laboratory has developed methods to prepare acyl carrier proteins (ACPs) loaded with substrate mimetics and cross-linkers to visualize and trap interactions with partner enzymes, and we continue to expand the tools for studying these pathways. We now describe application of the slow-onset, tight-binding inhibitor triclosan to explore the interactions between the type II fatty acid ACP from Escherichia coli, AcpP,, and its corresponding enoyl-ACP Teductase, FabI. We show that the AcpP-triclosan complex demonstrates nM binding, inhibits in vitro activity, and can be used to isolate FabI in complex proteomes.
An ideal target for metabolic engineering, fatty acid biosynthesis remains poorly understood on a molecular level. These carrier protein-dependent pathways require fundamental protein-protein interactions to guide reactivity and processivity, and their control has become one of the major hurdles in successfully adapting these biological machines. Our laboratory has developed methods to prepare acyl carrier proteins (ACPs) loaded with substrate mimetics and cross-linkers to visualize and trap interactions with partner enzymes, and we continue to expand the tools for studying these pathways. We now describe application of the slow-onset, tight-binding inhibitor triclosan to explore the interactions between the type II fatty acid ACP from Escherichia coli, AcpP, and its corresponding enoyl-ACP reductase, FabI. We show that the AcpP-triclosan complex demonstrates nM binding, inhibits in vitro activity, and can be used to isolate FabI in complex proteomes.
Author Finzel, Kara
Beld, Joris
Burkart, Michael D.
Tallorin, Lorillee
La Clair, James J.
Nguyen, Quynh G.
Author_xml – sequence: 1
  givenname: Lorillee
  surname: Tallorin
  fullname: Tallorin, Lorillee
  organization: Univ Calif San Diego, Dept Chem & Biochem, 9500 Gilman Dr, La Jolla, CA 92093 USA
– sequence: 2
  givenname: Kara
  surname: Finzel
  fullname: Finzel, Kara
  organization: Univ Calif San Diego, Dept Chem & Biochem, 9500 Gilman Dr, La Jolla, CA 92093 USA
– sequence: 3
  givenname: Quynh G.
  surname: Nguyen
  fullname: Nguyen, Quynh G.
  organization: Univ Calif San Diego, Dept Chem & Biochem, 9500 Gilman Dr, La Jolla, CA 92093 USA
– sequence: 4
  givenname: Joris
  surname: Beld
  fullname: Beld, Joris
  organization: Univ Calif San Diego, Dept Chem & Biochem, 9500 Gilman Dr, La Jolla, CA 92093 USA
– sequence: 5
  givenname: James J.
  surname: La Clair
  fullname: La Clair, James J.
  organization: Univ Calif San Diego, Dept Chem & Biochem, 9500 Gilman Dr, La Jolla, CA 92093 USA
– sequence: 6
  givenname: Michael D.
  surname: Burkart
  fullname: Burkart, Michael D.
  email: mburkart@ucsd.edu
  organization: Univ Calif San Diego, Dept Chem & Biochem, 9500 Gilman Dr, La Jolla, CA 92093 USA
BackLink https://www.ncbi.nlm.nih.gov/pubmed/26938266$$D View this record in MEDLINE/PubMed
BookMark eNqNkE1LAzEQQINU7IfePMseBdmaSZo0PcpStVBQpD0v2XRWt2yTNcki_fduafXkwbkMwzze4Q1JzzqLhFwDHQNlcL_VJoxFAXwi5BkZgGA0FcBkjwwopSydKsn7ZBjCtjsnTMEF6TM544pJOSDrlddNU9n3xJVJ_MBkbt2-Th_Mvk4y7X2FPnn1LmJlkzfctCbqgH-_Fzai1yZWzl6S81LXAa9Oe0TWj_NV9pwuX54W2cMy1QJkTGcGJFAOihWUG9SqQAFacSEkQMEVTnhBFQCHGcAMS8o5F1oJoxhyZJKNyO3R23j32WKI-a4KButaW3RtyEEBU4oKPu3QmxPaFjvc5I2vdtrv858UHXB3BL6wcGUwFVqDv1jXjk-5gM7WzUGn_k9nVdSHLJlrbWTfiS6BEQ
CitedBy_id crossref_primary_10_1016_j_molcel_2020_04_014
crossref_primary_10_3390_molecules22020235
crossref_primary_10_1002_cbic_202000747
crossref_primary_10_1016_j_bbamcr_2019_118540
crossref_primary_10_1016_j_chembiol_2016_08_014
crossref_primary_10_1038_s41467_020_15455_x
crossref_primary_10_1021_jacs_5c05635
crossref_primary_10_1038_s41467_025_63024_x
crossref_primary_10_1038_s41598_019_49261_3
crossref_primary_10_1002_psc_2943
crossref_primary_10_1016_j_cbpa_2018_06_004
crossref_primary_10_1111_cbdd_13851
Cites_doi 10.1016/j.bmc.2014.09.002
10.1371/journal.pone.0042949
10.1021/es702300s
10.1016/j.ab.2003.09.005
10.1016/j.chembiol.2004.02.010
10.1002/cbic.201402578
10.1039/b925966j
10.1039/b512735a
10.1021/cr040409x
10.1038/nrd1500
10.1021/ja4042059
10.1038/nature12810
10.1021/cb200004k
10.1110/ps.073288808
10.1021/bi0300229
10.1042/BJ20031821
10.1021/cb900099e
10.1021/jm030182i
10.1074/jbc.M309874200
10.1021/ar700156e
10.1074/jbc.M608758200
ContentType Journal Article
DBID 17B
1KM
BLEPL
DTL
EGQ
GYFQL
CGR
CUY
CVF
ECM
EIF
NPM
7X8
DOI 10.1021/jacs.5b13456
DatabaseName Web of Knowledge
Index Chemicus
Web of Science Core Collection
Science Citation Index Expanded
Web of Science Primary (SCIE, SSCI & AHCI)
Web of Science - Science Citation Index Expanded - 2016
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
MEDLINE - Academic
DatabaseTitle Web of Science
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
MEDLINE - Academic
DatabaseTitleList Web of Science
MEDLINE
MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: 1KM
  name: Index Chemicus
  url: https://www.webofscience.com/wos/woscc/search-with-editions?editions=WOS.IC
  sourceTypes:
    Enrichment Source
    Index Database
– sequence: 3
  dbid: 7X8
  name: MEDLINE - Academic
  url: https://search.proquest.com/medline
  sourceTypes: Aggregation Database
DeliveryMethod no_fulltext_linktorsrc
Discipline Chemistry
EISSN 1520-5126
EndPage 3965
ExternalDocumentID 26938266
000373518800007
Genre Research Support, U.S. Gov't, Non-P.H.S
Journal Article
Research Support, N.I.H., Extramural
GrantInformation_xml – fundername: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS)
  grantid: R01GM095970
– fundername: NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
  grantid: R01 GM095970; F31GM113470; IOS1516156
– fundername: NIGMS NIH HHS
  grantid: R01 GM095970
– fundername: NIGMS NIH HHS
  grantid: F31 GM113470
– fundername: NIGMS NIH HHS
  grantid: F31GM113470
GroupedDBID ---
-DZ
-ET
-~X
.DC
.K2
17B
1KM
4.4
53G
55A
5GY
5RE
5VS
7~N
85S
AABXI
AAHBH
AAYWT
ABBLG
ABJNI
ABLBI
ABMVS
ABPPZ
ABQRX
ABUCX
ABUFD
ACBEA
ACGFO
ACGFS
ACJ
ACNCT
ACS
ADHLV
AEESW
AENEX
AETEA
AFEFF
AGXLV
AHDLI
AHGAQ
ALMA_UNASSIGNED_HOLDINGS
AQSVZ
BAANH
BKOMP
BLEPL
CS3
CUPRZ
DTL
DU5
EBS
ED~
EJD
F5P
GGK
GNL
GROUPED_WOS_SCIENCE_CITATION_INDEX_EXPANDED
GROUPED_WOS_WEB_OF_SCIENCE
IH2
IH9
JG~
LG6
P2P
ROL
RXW
TAE
TN5
UHB
UI2
UKR
UPT
VF5
VG9
W1F
WH7
XSW
YQT
YZZ
ZCA
~02
CGR
CUY
CVF
ECM
EIF
NPM
7X8
ID FETCH-LOGICAL-a516t-9c16103182b03cea8be51a8355611b38e43b0811319119ef03335a85c82e3e262
IEDL.DBID 7X8
ISICitedReferencesCount 19
ISICitedReferencesURI https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestApp=WOS&DestLinkType=CitingArticles&UT=000373518800007
ISSN 0002-7863
IngestDate Sun Nov 09 10:40:08 EST 2025
Mon Jul 21 05:48:41 EDT 2025
Fri Dec 05 22:48:42 EST 2025
Mon Oct 27 21:55:09 EDT 2025
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 12
Keywords TARGET
TRICLOSAN
FABI
INHIBITION
FATTY-ACID BIOSYNTHESIS
MECHANISM
ANALOGS
PLASMODIUM-FALCIPARUM
ESCHERICHIA-COLI
BINDING
Language English
LinkModel DirectLink
LogoURL https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg
MergedId FETCHMERGED-LOGICAL-a516t-9c16103182b03cea8be51a8355611b38e43b0811319119ef03335a85c82e3e262
Notes NIH RePORTER
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
OpenAccessLink http://doi.org/10.1021/jacs.5b13456
PMID 26938266
PQID 1812880537
PQPubID 23479
PageCount 4
ParticipantIDs proquest_miscellaneous_1812880537
webofscience_primary_000373518800007
pubmed_primary_26938266
webofscience_primary_000373518800007CitationCount
PublicationCentury 2000
PublicationDate 2016-03-30
PublicationDateYYYYMMDD 2016-03-30
PublicationDate_xml – month: 03
  year: 2016
  text: 2016-03-30
  day: 30
PublicationDecade 2010
PublicationPlace WASHINGTON
PublicationPlace_xml – name: WASHINGTON
– name: United States
PublicationTitle Journal of the American Chemical Society
PublicationTitleAbbrev J AM CHEM SOC
PublicationTitleAlternate J Am Chem Soc
PublicationYear 2016
Publisher Amer Chemical Soc
Publisher_xml – name: Amer Chemical Soc
References Sivaraman, S (WOS:000182420000010) 2003; 42
ROCK, CO (WOS:A1982PH18200041) 1982; 257
Sivaraman, S (WOS:000188367800003) 2004; 47
Lu, H (WOS:000252419500003) 2008; 41
Rafi, S (WOS:000242898700035) 2006; 281
Ward, WHJ (WOS:000082782700037) 1999; 38
Rock, CO (WOS:A1996UY02100001) 1996; 1302
Heath, RJ (WOS:000079751900063) 1999; 274
Kapoor, M (WOS:000223440600018) 2004; 381
Nguyen, C (WOS:000329621800048) 2014; 505
Zhang, YM (WOS:000167474900078) 2001; 276
Worthington, AS (WOS:000276192100003) 2010; 8
Pan, P (WOS:000302501300002) 2012; 12
Prescott, D. J. (000373518800007.19) 2006; 36
Saito, J (WOS:000254197800011) 2008; 17
La Clair, JJ (WOS:000189284600010) 2004; 11
Ishikawa, F (WOS:000320899200021) 2013; 135
Brun, EM (WOS:000253521300047) 2008; 42
Qiu, XY (WOS:000083626200036) 1999; 8
Heath, RJ (WOS:000166039500046) 2000; 275
Keskin, O (WOS:000254855300002) 2008; 108
Tallorin, L (WOS:000344471800034) 2014; 22
Blatti, JL (WOS:000308788700006) 2012; 7
Swinney, DC (WOS:000223654800023) 2004; 3
Nazi, I (WOS:000187566500014) 2004; 324
Zhang, YM (WOS:000187480700116) 2003; 278
Arthur, CJ (WOS:000269087500005) 2009; 4
Bergler, H (WOS:A1996WB94900033) 1996; 242
HEATH, RJ (WOS:A1995TC97800080) 1995; 270
Heath, RJ (WOS:000077008100040) 1998; 273
Haushalter, RW (WOS:000290747200005) 2011; 6
Worthington, AS (WOS:000233968100008) 2006; 4
Finzel, K (WOS:000350138700001) 2015; 16
25676190 - Chembiochem. 2015 Mar 2;16(4):528-47
10196195 - J Biol Chem. 1999 Apr 16;274(16):11110-4
23028438 - PLoS One. 2012;7(9):e42949
11078736 - J Biol Chem. 2001 Mar 16;276(11):8231-8
18193820 - Acc Chem Res. 2008 Jan;41(1):11-20
15123281 - Chem Biol. 2004 Feb;11(2):195-201
17012233 - J Biol Chem. 2006 Dec 22;281(51):39285-93
6809756 - J Biol Chem. 1982 Sep 25;257(18):10759-65
15340390 - Nat Rev Drug Discov. 2004 Sep;3(9):801-8
10595560 - Protein Sci. 1999 Nov;8(11):2529-32
24362570 - Nature. 2014 Jan 16;505(7483):427-31
15086316 - Biochem J. 2004 Aug 1;381(Pt 3):719-24
22283812 - Curr Top Med Chem. 2012;12(7):672-93
14654051 - Anal Biochem. 2004 Jan 1;324(1):100-5
23718183 - J Am Chem Soc. 2013 Jun 19;135(24):8846-9
20449476 - Org Biomol Chem. 2010 Apr 21;8(8):1769-72
16357994 - Org Biomol Chem. 2006 Jan 7;4(1):44-6
21268653 - ACS Chem Biol. 2011 May 20;6(5):413-8
19555075 - ACS Chem Biol. 2009 Aug 21;4(8):625-36
9022698 - Eur J Biochem. 1996 Dec 15;242(3):689-94
11007778 - J Biol Chem. 2000 Dec 22;275(51):40128-33
7592873 - J Biol Chem. 1995 Nov 3;270(44):26538-42
10493822 - Biochemistry. 1999 Sep 21;38(38):12514-25
14736233 - J Med Chem. 2004 Jan 29;47(3):509-18
18441818 - Environ Sci Technol. 2008 Mar 1;42(5):1665-72
14527946 - J Biol Chem. 2003 Dec 26;278(52):52935-43
8695652 - Biochim Biophys Acta. 1996 Jul 12;1302(1):1-16
4561013 - Adv Enzymol Relat Areas Mol Biol. 1972;36:269-311
9804793 - J Biol Chem. 1998 Nov 13;273(46):30316-20
18305197 - Protein Sci. 2008 Apr;17(4):691-9
18355092 - Chem Rev. 2008 Apr;108(4):1225-44
25284249 - Bioorg Med Chem. 2014 Nov 1;22(21):6053-61
12693936 - Biochemistry. 2003 Apr 22;42(15):4406-13
References_xml – volume: 22
  start-page: 6053
  year: 2014
  ident: WOS:000344471800034
  article-title: Celastrol inhibits Plasmodium falciparum enoyl-acyl carrier protein reductase
  publication-title: BIOORGANIC & MEDICINAL CHEMISTRY
  doi: 10.1016/j.bmc.2014.09.002
– volume: 7
  start-page: ARTN e42949
  year: 2012
  ident: WOS:000308788700006
  article-title: Manipulating Fatty Acid Biosynthesis in Microalgae for Biofuel through Protein-Protein Interactions
  publication-title: PLOS ONE
  doi: 10.1371/journal.pone.0042949
– volume: 42
  start-page: 1665
  year: 2008
  ident: WOS:000253521300047
  article-title: Selective enzyme-linked Immunosorbent assay for triclosan. Application to wastewater treatment plant effluents
  publication-title: ENVIRONMENTAL SCIENCE & TECHNOLOGY
  doi: 10.1021/es702300s
– volume: 324
  start-page: 100
  year: 2004
  ident: WOS:000187566500014
  article-title: One-pot chemoenzymatic preparation of coenzyme A analogues
  publication-title: ANALYTICAL BIOCHEMISTRY
  doi: 10.1016/j.ab.2003.09.005
– volume: 11
  start-page: 195
  year: 2004
  ident: WOS:000189284600010
  article-title: Manipulation of carrier proteins in antibiotic biosynthesis
  publication-title: CHEMISTRY & BIOLOGY
  doi: 10.1016/j.chembiol.2004.02.010
– volume: 16
  start-page: 528
  year: 2015
  ident: WOS:000350138700001
  article-title: Using Modern Tools To Probe the Structure-Function Relationship of Fatty Acid Synthases
  publication-title: CHEMBIOCHEM
  doi: 10.1002/cbic.201402578
– volume: 8
  start-page: 1769
  year: 2010
  ident: WOS:000276192100003
  article-title: Mechanism-based crosslinking as a gauge for functional interaction of modular synthases
  publication-title: ORGANIC & BIOMOLECULAR CHEMISTRY
  doi: 10.1039/b925966j
– volume: 270
  start-page: 26538
  year: 1995
  ident: WOS:A1995TC97800080
  article-title: ENOYL-ACYL CARRIER PROTEIN REDUCTASE (FABI) PLAYS A DETERMINANT ROLE IN COMPLETING CYCLES OF FATTY-ACID ELONGATION IN ESCHERICHIA-COLI
  publication-title: JOURNAL OF BIOLOGICAL CHEMISTRY
– volume: 12
  start-page: 672
  year: 2012
  ident: WOS:000302501300002
  article-title: Targeting InhA, the FASII Enoyl-ACP Reductase: SAR Studies on Novel Inhibitor Scaffolds
  publication-title: CURRENT TOPICS IN MEDICINAL CHEMISTRY
– volume: 36
  start-page: 269
  year: 2006
  ident: 000373518800007.19
  publication-title: Adv. Enzymol. Relat. Areas Mol. Biol.
– volume: 273
  start-page: 30316
  year: 1998
  ident: WOS:000077008100040
  article-title: Broad spectrum antimicrobial biocides target the FabI component of fatty acid synthesis
  publication-title: JOURNAL OF BIOLOGICAL CHEMISTRY
– volume: 4
  start-page: 44
  year: 2006
  ident: WOS:000233968100008
  article-title: One-pot chemo-enzymatic synthesis of reporter-modified proteins
  publication-title: ORGANIC & BIOMOLECULAR CHEMISTRY
  doi: 10.1039/b512735a
– volume: 1302
  start-page: 1
  year: 1996
  ident: WOS:A1996UY02100001
  article-title: Escherichia coli as a model for the regulation of dissociable (type II) fatty acid biosynthesis
  publication-title: BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM
– volume: 108
  start-page: 1225
  year: 2008
  ident: WOS:000254855300002
  article-title: Principles of protein-protein interactions: What are the preferred ways for proteins to interact?
  publication-title: CHEMICAL REVIEWS
  doi: 10.1021/cr040409x
– volume: 276
  start-page: 8231
  year: 2001
  ident: WOS:000167474900078
  article-title: Identification and analysis of the acyl carrier protein (ACP) docking site on beta-ketoacyl-ACP synthase III
  publication-title: JOURNAL OF BIOLOGICAL CHEMISTRY
– volume: 3
  start-page: 801
  year: 2004
  ident: WOS:000223654800023
  article-title: Biochemical mechanisms of drug action: what does it take for success?
  publication-title: NATURE REVIEWS DRUG DISCOVERY
  doi: 10.1038/nrd1500
– volume: 242
  start-page: 689
  year: 1996
  ident: WOS:A1996WB94900033
  article-title: The enoyl-[acyl-carrier-protein] reductase (FabI) of Escherichia coli, which catalyzes a key regulatory step in fatty acid biosynthesis, accepts NADH and NADPH as cofactors and is inhibited by palmitoyl-CoA
  publication-title: EUROPEAN JOURNAL OF BIOCHEMISTRY
– volume: 135
  start-page: 8846
  year: 2013
  ident: WOS:000320899200021
  article-title: Sulfonyl 3-Alkynyi Pantetheinamides as Mechanism-Based Cross-Linkers of Acyl Carrier Protein Dehydratase
  publication-title: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
  doi: 10.1021/ja4042059
– volume: 505
  start-page: 427
  year: 2014
  ident: WOS:000329621800048
  article-title: Trapping the dynamic acyl carrier protein in fatty acid biosynthesis
  publication-title: NATURE
  doi: 10.1038/nature12810
– volume: 274
  start-page: 11110
  year: 1999
  ident: WOS:000079751900063
  article-title: Mechanism of triclosan inhibition of bacterial fatty acid synthesis
  publication-title: JOURNAL OF BIOLOGICAL CHEMISTRY
– volume: 6
  start-page: 413
  year: 2011
  ident: WOS:000290747200005
  article-title: Binding and pK(a) Modulation of a Polycyclic Substrate Analogue in a Type II Polyketide Acyl Carrier Protein
  publication-title: ACS CHEMICAL BIOLOGY
  doi: 10.1021/cb200004k
– volume: 17
  start-page: 691
  year: 2008
  ident: WOS:000254197800011
  article-title: Crystal structure of enoyl-acyl carrier protein reductase (FabK) from Streptococcus pneumoniae reveals the binding mode of an inhibitor
  publication-title: PROTEIN SCIENCE
  doi: 10.1110/ps.073288808
– volume: 42
  start-page: 4406
  year: 2003
  ident: WOS:000182420000010
  article-title: Structure-activity studies of the inhibition of FabI, the enoyl reductase from Escherichia coli, by triclosan: Kinetic analysis of mutant
  publication-title: BIOCHEMISTRY
  doi: 10.1021/bi0300229
– volume: 381
  start-page: 719
  year: 2004
  ident: WOS:000223440600018
  article-title: Slow-tight-binding inhibition of enoyl-acyl carrier protein reductase from Plasmodium falciparum by triclosan
  publication-title: BIOCHEMICAL JOURNAL
  doi: 10.1042/BJ20031821
– volume: 257
  start-page: 10759
  year: 1982
  ident: WOS:A1982PH18200041
  article-title: REGULATION OF PHOSPHOLIPID-SYNTHESIS IN ESCHERICHIA-COLI - COMPOSITION OF THE ACYL-ACYL CARRIER PROTEIN POOL INVIVO
  publication-title: JOURNAL OF BIOLOGICAL CHEMISTRY
– volume: 8
  start-page: 2529
  year: 1999
  ident: WOS:000083626200036
  article-title: Molecular basis for triclosan activity involves a flipping loop in the active site
  publication-title: PROTEIN SCIENCE
– volume: 4
  start-page: 625
  year: 2009
  ident: WOS:000269087500005
  article-title: Structure and Malonyl CoA-ACP Transacylase Binding of Streptomyces coelicolor Fatty Acid Synthase Acyl Carrier Protein
  publication-title: ACS CHEMICAL BIOLOGY
  doi: 10.1021/cb900099e
– volume: 47
  start-page: 509
  year: 2004
  ident: WOS:000188367800003
  article-title: Inhibition of the bacterial enoyl reductase FabI by triclosan: A structure-reactivity analysis of FabI inhibition by triclosan analogues
  publication-title: JOURNAL OF MEDICINAL CHEMISTRY
  doi: 10.1021/jm030182i
– volume: 278
  start-page: 52935
  year: 2003
  ident: WOS:000187480700116
  article-title: Key residues responsible for acyl carrier protein and beta-ketoacyl-acyl carrier protein reductase (FabG) interaction
  publication-title: JOURNAL OF BIOLOGICAL CHEMISTRY
  doi: 10.1074/jbc.M309874200
– volume: 41
  start-page: 11
  year: 2008
  ident: WOS:000252419500003
  article-title: Inhibitors of Fabl, an enzyme drug target in the bacterial fatty acid biosynthesis pathway
  publication-title: ACCOUNTS OF CHEMICAL RESEARCH
  doi: 10.1021/ar700156e
– volume: 38
  start-page: 12514
  year: 1999
  ident: WOS:000082782700037
  article-title: Kinetic and structural characteristics of the inhibition of enoyl (acyl carrier protein) reductase by triclosan
  publication-title: BIOCHEMISTRY
– volume: 275
  start-page: 40128
  year: 2000
  ident: WOS:000166039500046
  article-title: The enoyl-[acyl-carrier-protein] reductases FabI and FabL from Bacillus subtilis
  publication-title: JOURNAL OF BIOLOGICAL CHEMISTRY
– volume: 281
  start-page: 39285
  year: 2006
  ident: WOS:000242898700035
  article-title: Structure of acyl carrier protein bound to FabI, the FASII enoyl reductase from Escherichia coli
  publication-title: JOURNAL OF BIOLOGICAL CHEMISTRY
  doi: 10.1074/jbc.M608758200
– reference: 14654051 - Anal Biochem. 2004 Jan 1;324(1):100-5
– reference: 18441818 - Environ Sci Technol. 2008 Mar 1;42(5):1665-72
– reference: 8695652 - Biochim Biophys Acta. 1996 Jul 12;1302(1):1-16
– reference: 10493822 - Biochemistry. 1999 Sep 21;38(38):12514-25
– reference: 21268653 - ACS Chem Biol. 2011 May 20;6(5):413-8
– reference: 11007778 - J Biol Chem. 2000 Dec 22;275(51):40128-33
– reference: 20449476 - Org Biomol Chem. 2010 Apr 21;8(8):1769-72
– reference: 25284249 - Bioorg Med Chem. 2014 Nov 1;22(21):6053-61
– reference: 9022698 - Eur J Biochem. 1996 Dec 15;242(3):689-94
– reference: 15086316 - Biochem J. 2004 Aug 1;381(Pt 3):719-24
– reference: 24362570 - Nature. 2014 Jan 16;505(7483):427-31
– reference: 16357994 - Org Biomol Chem. 2006 Jan 7;4(1):44-6
– reference: 18305197 - Protein Sci. 2008 Apr;17(4):691-9
– reference: 9804793 - J Biol Chem. 1998 Nov 13;273(46):30316-20
– reference: 7592873 - J Biol Chem. 1995 Nov 3;270(44):26538-42
– reference: 19555075 - ACS Chem Biol. 2009 Aug 21;4(8):625-36
– reference: 18355092 - Chem Rev. 2008 Apr;108(4):1225-44
– reference: 10196195 - J Biol Chem. 1999 Apr 16;274(16):11110-4
– reference: 10595560 - Protein Sci. 1999 Nov;8(11):2529-32
– reference: 12693936 - Biochemistry. 2003 Apr 22;42(15):4406-13
– reference: 11078736 - J Biol Chem. 2001 Mar 16;276(11):8231-8
– reference: 4561013 - Adv Enzymol Relat Areas Mol Biol. 1972;36:269-311
– reference: 25676190 - Chembiochem. 2015 Mar 2;16(4):528-47
– reference: 23718183 - J Am Chem Soc. 2013 Jun 19;135(24):8846-9
– reference: 18193820 - Acc Chem Res. 2008 Jan;41(1):11-20
– reference: 14527946 - J Biol Chem. 2003 Dec 26;278(52):52935-43
– reference: 6809756 - J Biol Chem. 1982 Sep 25;257(18):10759-65
– reference: 15123281 - Chem Biol. 2004 Feb;11(2):195-201
– reference: 15340390 - Nat Rev Drug Discov. 2004 Sep;3(9):801-8
– reference: 17012233 - J Biol Chem. 2006 Dec 22;281(51):39285-93
– reference: 23028438 - PLoS One. 2012;7(9):e42949
– reference: 14736233 - J Med Chem. 2004 Jan 29;47(3):509-18
– reference: 22283812 - Curr Top Med Chem. 2012;12(7):672-93
SSID ssj0004281
Score 2.2948878
Snippet An ideal target for metabolic engineering, fatty acid biosynthesis remains poorly understood on a molecular level. These carrier protein-dependent pathways...
Source Web of Science
SourceID proquest
pubmed
webofscience
SourceType Aggregation Database
Index Database
Enrichment Source
StartPage 3962
SubjectTerms Acyl Carrier Protein - metabolism
Chemistry
Chemistry, Multidisciplinary
Enoyl-(Acyl-Carrier-Protein) Reductase (NADH) - isolation & purification
Enoyl-(Acyl-Carrier-Protein) Reductase (NADH) - metabolism
Escherichia coli - enzymology
Escherichia coli Proteins - isolation & purification
Escherichia coli Proteins - metabolism
Fatty Acid Synthase, Type II - isolation & purification
Fatty Acid Synthase, Type II - metabolism
Models, Molecular
Molecular Structure
Oxidoreductases - isolation & purification
Oxidoreductases - metabolism
Physical Sciences
Protein Binding
Protein Engineering
Science & Technology
Triclosan - chemistry
Triclosan - metabolism
Title Trapping of the Enoyl-Acyl Carrier Protein Reductase-Acyl Carrier Protein Interaction
URI http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestApp=WOS&DestLinkType=FullRecord&UT=000373518800007
https://www.ncbi.nlm.nih.gov/pubmed/26938266
https://www.proquest.com/docview/1812880537
Volume 138
WOS 000373518800007
WOSCitedRecordID wos000373518800007
hasFullText
inHoldings 1
isFullTextHit
isPrint
link http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LS8NAEB7UCnrx_YiPEqHX2Gw2m8dJSrAI2lLEQm9hd7OVSklqE4X-e2eTVHvooeAllw1hszM7883szjcALTm2hUgkxbBESsvlKAsumbRo4jImfelxUUr6xe_3g9EoHNQJt7y-Vrm0iaWhTjKpc-Rt7YlQ1xj1H2aflu4apU9X6xYa29CgCGW0VvujFbZwJyD1ZXd0Ze0PLvN7Jgh1mbcOUK71PaWf6R7-d4ZHcFAjTLNTqcQxbKn0BPaiZWO3Uxiif9KsDO9mNjYR_5mPabaYWh25mJoRn-sWduZA0zdMUvNVM7sW6OnWD5f5xKo04gyG3ce36MmquytYnBGvsEKJYE9vaUfYVCoeCMUIR0CGiIoIGiiXCsQLBPcoIaEa25RSxgMmA0dR5XjOOeykWaouwUQjwHVck9ghd72AC1sQ_SHmY_gp3MCAu-WKxfiv-kiCpyr7yuO_NTPgopJCPKtoNmLHCykGP54BrVWx_I6X3Dm0JJTTSMcAsslrUU19rkv-i6sNJnYN-wiSvLIO0b6BxhjNgrqFXfldTPJ5EyH5c69Z6h0--4PeDyo14WE
linkProvider ProQuest
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Trapping+of+the+Enoyl-Acyl+Carrier+Protein+Reductase-Acyl+Carrier+Protein+Interaction&rft.jtitle=Journal+of+the+American+Chemical+Society&rft.au=Tallorin%2C+Lorillee&rft.au=Finzel%2C+Kara&rft.au=Nguyen%2C+Quynh+G&rft.au=Beld%2C+Joris&rft.date=2016-03-30&rft.eissn=1520-5126&rft.volume=138&rft.issue=12&rft.spage=3962&rft_id=info:doi/10.1021%2Fjacs.5b13456&rft_id=info%3Apmid%2F26938266&rft_id=info%3Apmid%2F26938266&rft.externalDocID=26938266
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0002-7863&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0002-7863&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0002-7863&client=summon