Particles for multiplexed analysis in solution: detection and identification of striped metallic particles using optical microscopy

In this report, we present data demonstrating that cylindrical metallic particles, with various submicrometer striping patterns, may be readily distinguished in an optical microscope. Accurate particle identification is discussed relative to synthesis reproducibility and the limitations of optical m...

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Bibliographic Details
Published in:	Analytical chemistry (Washington) Vol. 74; no. 10; p. 2240
Main Authors:	Walton, Ian D, Norton, Scott M, Balasingham, Arjuna, He, Lin, Oviso, Jr, Dominador F, Gupta, Dimpy, Raju, Paul A, Natan, Michael J, Freeman, R Griffith
Format:	Journal Article
Language:	English
Published:	United States 15.05.2002
Subjects:	Diagnostic Imaging - standards Electronic Data Processing - instrumentation Electronic Data Processing - methods Electronic Data Processing - standards Metals - standards Microscopy - methods Nanotechnology Particle Size
ISSN:	0003-2700
Online Access:	Get more information
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Summary:	In this report, we present data demonstrating that cylindrical metallic particles, with various submicrometer striping patterns, may be readily distinguished in an optical microscope. Accurate particle identification is discussed relative to synthesis reproducibility and the limitations of optical microscopes. Results from a library of these particles, of which over 100 different striping patterns have been produced, are presented. For these particles, made with Au and Ag stripes, more than 70 patterns may be identified with greater than 90% accuracy. The ability to chemically modify the surface of these particles, making them useful for bioanalytical measurements, is also demonstrated. Finally, we discuss improvements in our manufacturing and identification processes that will lead to both larger numbers of striping patterns and improved identification accuracy.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0003-2700
DOI:	10.1021/ac020073w