Diagnosis of Human Prion Disease Using Real-Time Quaking-Induced Conversion Testing of Olfactory Mucosa and Cerebrospinal Fluid Samples
Early and accurate in vivo diagnosis of Creutzfeldt-Jakob disease (CJD) is necessary for quickly distinguishing treatable from untreatable rapidly progressive dementias and for future therapeutic trials. This early diagnosis is becoming possible using the real-time quaking-induced conversion (RT-QuI...
Saved in:
| Published in: | JAMA neurology Vol. 74; no. 2; p. 155 |
|---|---|
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
01.02.2017
|
| Subjects: | |
| ISSN: | 2168-6157, 2168-6157 |
| Online Access: | Get more information |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Early and accurate in vivo diagnosis of Creutzfeldt-Jakob disease (CJD) is necessary for quickly distinguishing treatable from untreatable rapidly progressive dementias and for future therapeutic trials. This early diagnosis is becoming possible using the real-time quaking-induced conversion (RT-QuIC) seeding assay, which detects minute amounts of the disease-specific pathologic prion protein in cerebrospinal fluid (CSF) or olfactory mucosa (OM) samples.
To develop an algorithm for accurate and early diagnosis of CJD by using the RT-QuIC assay on CSF samples, OM samples, or both.
In this case-control study, samples of CSF and OM were collected from 86 patients with a clinical diagnosis of probable (n = 51), possible (n = 24), or suspected (n = 11) CJD and 104 negative control samples (54 CSF and 50 OM). The CSF and OM samples were analyzed using conventional RT-QuIC. The CSF samples underwent further testing using improved RT-QuIC conditions. In addition, the diagnostic performance of a novel, easy-to-use, gentle flocked swab for sampling of OM was evaluated. Data were collected from January 1 to June 30, 2015.
Correlations between RT-QuIC results and the final diagnosis of recruited patients.
Among the 86 patients (37 men [43%] and 49 women [57%]; mean [SD] age, 65.7 [11.5] years) included for analysis, all 61 patients with sporadic CJD had positive RT-QuIC findings using OM or CSF samples or both for an overall RT-QuIC diagnostic sensitivity of 100% (95% CI, 93%-100%). All patients with a final diagnosis of non-prion disease (71 CSF and 67 OM samples) had negative RT-QuIC findings for 100% specificity (95% CI, 94%-100%). Of 8 symptomatic patients with various mutations causing CJD or Gerstmann-Sträussler-Scheinker syndrome, 6 had positive and 2 had negative RT-QuIC findings for a sensitivity of 75% (95% CI, 36%-96%).
A proposed diagnostic algorithm for sporadic CJD combines CSF and OM RT-QuIC testing to provide virtually 100% diagnostic sensitivity and specificity in the clinical phase of the disease. |
|---|---|
| AbstractList | Early and accurate in vivo diagnosis of Creutzfeldt-Jakob disease (CJD) is necessary for quickly distinguishing treatable from untreatable rapidly progressive dementias and for future therapeutic trials. This early diagnosis is becoming possible using the real-time quaking-induced conversion (RT-QuIC) seeding assay, which detects minute amounts of the disease-specific pathologic prion protein in cerebrospinal fluid (CSF) or olfactory mucosa (OM) samples.
To develop an algorithm for accurate and early diagnosis of CJD by using the RT-QuIC assay on CSF samples, OM samples, or both.
In this case-control study, samples of CSF and OM were collected from 86 patients with a clinical diagnosis of probable (n = 51), possible (n = 24), or suspected (n = 11) CJD and 104 negative control samples (54 CSF and 50 OM). The CSF and OM samples were analyzed using conventional RT-QuIC. The CSF samples underwent further testing using improved RT-QuIC conditions. In addition, the diagnostic performance of a novel, easy-to-use, gentle flocked swab for sampling of OM was evaluated. Data were collected from January 1 to June 30, 2015.
Correlations between RT-QuIC results and the final diagnosis of recruited patients.
Among the 86 patients (37 men [43%] and 49 women [57%]; mean [SD] age, 65.7 [11.5] years) included for analysis, all 61 patients with sporadic CJD had positive RT-QuIC findings using OM or CSF samples or both for an overall RT-QuIC diagnostic sensitivity of 100% (95% CI, 93%-100%). All patients with a final diagnosis of non-prion disease (71 CSF and 67 OM samples) had negative RT-QuIC findings for 100% specificity (95% CI, 94%-100%). Of 8 symptomatic patients with various mutations causing CJD or Gerstmann-Sträussler-Scheinker syndrome, 6 had positive and 2 had negative RT-QuIC findings for a sensitivity of 75% (95% CI, 36%-96%).
A proposed diagnostic algorithm for sporadic CJD combines CSF and OM RT-QuIC testing to provide virtually 100% diagnostic sensitivity and specificity in the clinical phase of the disease. Early and accurate in vivo diagnosis of Creutzfeldt-Jakob disease (CJD) is necessary for quickly distinguishing treatable from untreatable rapidly progressive dementias and for future therapeutic trials. This early diagnosis is becoming possible using the real-time quaking-induced conversion (RT-QuIC) seeding assay, which detects minute amounts of the disease-specific pathologic prion protein in cerebrospinal fluid (CSF) or olfactory mucosa (OM) samples.ImportanceEarly and accurate in vivo diagnosis of Creutzfeldt-Jakob disease (CJD) is necessary for quickly distinguishing treatable from untreatable rapidly progressive dementias and for future therapeutic trials. This early diagnosis is becoming possible using the real-time quaking-induced conversion (RT-QuIC) seeding assay, which detects minute amounts of the disease-specific pathologic prion protein in cerebrospinal fluid (CSF) or olfactory mucosa (OM) samples.To develop an algorithm for accurate and early diagnosis of CJD by using the RT-QuIC assay on CSF samples, OM samples, or both.ObjectiveTo develop an algorithm for accurate and early diagnosis of CJD by using the RT-QuIC assay on CSF samples, OM samples, or both.In this case-control study, samples of CSF and OM were collected from 86 patients with a clinical diagnosis of probable (n = 51), possible (n = 24), or suspected (n = 11) CJD and 104 negative control samples (54 CSF and 50 OM). The CSF and OM samples were analyzed using conventional RT-QuIC. The CSF samples underwent further testing using improved RT-QuIC conditions. In addition, the diagnostic performance of a novel, easy-to-use, gentle flocked swab for sampling of OM was evaluated. Data were collected from January 1 to June 30, 2015.Design, Setting, and ParticipantsIn this case-control study, samples of CSF and OM were collected from 86 patients with a clinical diagnosis of probable (n = 51), possible (n = 24), or suspected (n = 11) CJD and 104 negative control samples (54 CSF and 50 OM). The CSF and OM samples were analyzed using conventional RT-QuIC. The CSF samples underwent further testing using improved RT-QuIC conditions. In addition, the diagnostic performance of a novel, easy-to-use, gentle flocked swab for sampling of OM was evaluated. Data were collected from January 1 to June 30, 2015.Correlations between RT-QuIC results and the final diagnosis of recruited patients.Main Outcome and MeasuresCorrelations between RT-QuIC results and the final diagnosis of recruited patients.Among the 86 patients (37 men [43%] and 49 women [57%]; mean [SD] age, 65.7 [11.5] years) included for analysis, all 61 patients with sporadic CJD had positive RT-QuIC findings using OM or CSF samples or both for an overall RT-QuIC diagnostic sensitivity of 100% (95% CI, 93%-100%). All patients with a final diagnosis of non-prion disease (71 CSF and 67 OM samples) had negative RT-QuIC findings for 100% specificity (95% CI, 94%-100%). Of 8 symptomatic patients with various mutations causing CJD or Gerstmann-Sträussler-Scheinker syndrome, 6 had positive and 2 had negative RT-QuIC findings for a sensitivity of 75% (95% CI, 36%-96%).ResultsAmong the 86 patients (37 men [43%] and 49 women [57%]; mean [SD] age, 65.7 [11.5] years) included for analysis, all 61 patients with sporadic CJD had positive RT-QuIC findings using OM or CSF samples or both for an overall RT-QuIC diagnostic sensitivity of 100% (95% CI, 93%-100%). All patients with a final diagnosis of non-prion disease (71 CSF and 67 OM samples) had negative RT-QuIC findings for 100% specificity (95% CI, 94%-100%). Of 8 symptomatic patients with various mutations causing CJD or Gerstmann-Sträussler-Scheinker syndrome, 6 had positive and 2 had negative RT-QuIC findings for a sensitivity of 75% (95% CI, 36%-96%).A proposed diagnostic algorithm for sporadic CJD combines CSF and OM RT-QuIC testing to provide virtually 100% diagnostic sensitivity and specificity in the clinical phase of the disease.Conclusions and RelevanceA proposed diagnostic algorithm for sporadic CJD combines CSF and OM RT-QuIC testing to provide virtually 100% diagnostic sensitivity and specificity in the clinical phase of the disease. |
| Author | Monaco, Salvatore Testi, Silvia Imperiale, Daniele Cattaruzza, Tatiana Tiple, Dorina Caughey, Byron Capaldi, Stefano Poleggi, Anna Fiorini, Michele Ferrari, Sergio Ladogana, Anna Orrù, Christina Tonoli, Giovanni Triva, Giorgio Cagnin, Annachiara Castriciano, Santina Marchioni, Daniele Pocchiari, Maurizio Zanusso, Gianluigi Colaizzo, Elisa Bongianni, Matilde Sacchetto, Luca Hughson, Andrew G Groveman, Bradley R Fabrizi, Gian Maria Vaianella, Luana |
| Author_xml | – sequence: 1 givenname: Matilde surname: Bongianni fullname: Bongianni, Matilde organization: Department of Neurosciences, Biomedicine, and Movement Sciences, University of Verona, Policlinico G. B. Rossi, Verona, Italy – sequence: 2 givenname: Christina surname: Orrù fullname: Orrù, Christina organization: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute for Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana – sequence: 3 givenname: Bradley R surname: Groveman fullname: Groveman, Bradley R organization: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute for Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana – sequence: 4 givenname: Luca surname: Sacchetto fullname: Sacchetto, Luca organization: Department of Surgical Sciences, Dentistry, Gynecology, and Pediatrics, University of Verona, Verona, Italy – sequence: 5 givenname: Michele surname: Fiorini fullname: Fiorini, Michele organization: Department of Neurosciences, Biomedicine, and Movement Sciences, University of Verona, Policlinico G. B. Rossi, Verona, Italy – sequence: 6 givenname: Giovanni surname: Tonoli fullname: Tonoli, Giovanni organization: Struttura Complessa di Otorinolaringoiatria, Ospedale Santa Maria della Misericordia, Rovigo, Italy – sequence: 7 givenname: Giorgio surname: Triva fullname: Triva, Giorgio organization: Copan Italia SpA, Brescia, Italy – sequence: 8 givenname: Stefano surname: Capaldi fullname: Capaldi, Stefano organization: Biocrystallography Laboratory, Department of Biotechnology, University of Verona, Italy – sequence: 9 givenname: Silvia surname: Testi fullname: Testi, Silvia organization: Department of Neurosciences, Biomedicine, and Movement Sciences, University of Verona, Policlinico G. B. Rossi, Verona, Italy – sequence: 10 givenname: Sergio surname: Ferrari fullname: Ferrari, Sergio organization: Department of Neurosciences, Biomedicine, and Movement Sciences, University of Verona, Policlinico G. B. Rossi, Verona, Italy – sequence: 11 givenname: Annachiara surname: Cagnin fullname: Cagnin, Annachiara organization: Department of Neuroscience, University of Padova, Padova, Italy8Istituto di Ricovero e Cura a Carattere Scientifico San Camillo Hospital, Venice, Italy – sequence: 12 givenname: Anna surname: Ladogana fullname: Ladogana, Anna organization: Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy – sequence: 13 givenname: Anna surname: Poleggi fullname: Poleggi, Anna organization: Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy – sequence: 14 givenname: Elisa surname: Colaizzo fullname: Colaizzo, Elisa organization: Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy – sequence: 15 givenname: Dorina surname: Tiple fullname: Tiple, Dorina organization: Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy – sequence: 16 givenname: Luana surname: Vaianella fullname: Vaianella, Luana organization: Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy – sequence: 17 givenname: Santina surname: Castriciano fullname: Castriciano, Santina organization: Copan Italia SpA, Brescia, Italy – sequence: 18 givenname: Daniele surname: Marchioni fullname: Marchioni, Daniele organization: Department of Surgical Sciences, Dentistry, Gynecology, and Pediatrics, University of Verona, Verona, Italy – sequence: 19 givenname: Andrew G surname: Hughson fullname: Hughson, Andrew G organization: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute for Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana – sequence: 20 givenname: Daniele surname: Imperiale fullname: Imperiale, Daniele organization: Neurology Unit, Ospedale Maria Vittoria, Torino, Italy – sequence: 21 givenname: Tatiana surname: Cattaruzza fullname: Cattaruzza, Tatiana organization: Neurology Unit, Ospedale Cattinara, Trieste, Italy – sequence: 22 givenname: Gian Maria surname: Fabrizi fullname: Fabrizi, Gian Maria organization: Department of Neurosciences, Biomedicine, and Movement Sciences, University of Verona, Policlinico G. B. Rossi, Verona, Italy – sequence: 23 givenname: Maurizio surname: Pocchiari fullname: Pocchiari, Maurizio organization: Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy – sequence: 24 givenname: Salvatore surname: Monaco fullname: Monaco, Salvatore organization: Department of Neurosciences, Biomedicine, and Movement Sciences, University of Verona, Policlinico G. B. Rossi, Verona, Italy – sequence: 25 givenname: Byron surname: Caughey fullname: Caughey, Byron organization: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute for Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana – sequence: 26 givenname: Gianluigi surname: Zanusso fullname: Zanusso, Gianluigi organization: Department of Neurosciences, Biomedicine, and Movement Sciences, University of Verona, Policlinico G. B. Rossi, Verona, Italy |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/27942718$$D View this record in MEDLINE/PubMed |
| BookMark | eNpNkNtKAzEQhoMoVmvfQCSX3mzNYY-X0nooVOqhvV5md2dLajapSSP0CXxtt6jg3MwwfPMx_Ofk2FiDhFxyNuaM8ZsNdGAwOKvHgvF0HKc8PiJngqd5lPIkO_43D8jI-w3rK2cslvEpGYisiEXG8zPyNVWwNtYrT21LH0Ovpc9OWUOnyiN4pCuvzJq-IuhoqTqkLwHe-000M02osaETaz7R-cPJEv3uAPemhW6h3lm3p0-hth4omB5Fh5WzfqsMaHqvg2roG3Rbjf6CnLSgPY5--5Cs7u-Wk8dovniYTW7nEcQZ30WyqmWRxTyFjLV1BlUjkwarvG1bKXOGohENSsaSpMCaJzKvEXghWygAKuBcDMn1j3fr7Efo_y075WvUuo_TBl_yPBFpGhcs7dGrXzRUHTbl1qkO3L78C098A1WSeGY |
| CitedBy_id | crossref_primary_10_1016_j_mcn_2018_12_003 crossref_primary_10_1177_1941874419846338 crossref_primary_10_1038_s41598_021_84527_9 crossref_primary_10_1111_ene_15258 crossref_primary_10_3390_pathogens10030305 crossref_primary_10_3389_fnagi_2022_874734 crossref_primary_10_1080_14737175_2018_1519397 crossref_primary_10_1007_s00441_022_03595_z crossref_primary_10_1002_alz_13541 crossref_primary_10_3390_v13050759 crossref_primary_10_1073_pnas_1909322116 crossref_primary_10_3390_pathogens10060750 crossref_primary_10_1097_QAD_0000000000002102 crossref_primary_10_1212_WNL_0000000000006724 crossref_primary_10_1002_ccr3_3131 crossref_primary_10_1186_s40478_018_0508_2 crossref_primary_10_1007_s00401_017_1692_z crossref_primary_10_3390_biom10091233 crossref_primary_10_1212_WNL_0000000000005860 crossref_primary_10_1016_j_pathol_2024_09_005 crossref_primary_10_1038_s41380_021_01045_w crossref_primary_10_1186_s40478_020_00990_x crossref_primary_10_1126_scitranslmed_aat8462 crossref_primary_10_12688_f1000research_12681_1 crossref_primary_10_1007_s00401_023_02661_2 crossref_primary_10_1111_neup_12557 crossref_primary_10_1001_jamaophthalmol_2020_0447 crossref_primary_10_1007_s00441_021_03568_8 crossref_primary_10_1016_j_nucmedbio_2020_09_003 crossref_primary_10_3390_biom12040576 crossref_primary_10_1016_j_neurol_2019_06_002 crossref_primary_10_1371_journal_pone_0225904 crossref_primary_10_1016_j_ncl_2018_07_005 crossref_primary_10_1111_ene_15795 crossref_primary_10_1002_acn3_51000 crossref_primary_10_3389_fnagi_2021_703984 crossref_primary_10_1007_s00415_022_11549_2 crossref_primary_10_1002_mdc3_13737 crossref_primary_10_1016_j_mam_2017_11_011 crossref_primary_10_1007_s00415_023_11962_1 crossref_primary_10_1007_s10072_017_2968_8 crossref_primary_10_1016_j_conb_2020_01_009 crossref_primary_10_3389_fneur_2020_00763 crossref_primary_10_1007_s00401_019_02080_2 crossref_primary_10_1093_brain_awab005 crossref_primary_10_1080_14737175_2023_2246653 crossref_primary_10_3390_v11040309 crossref_primary_10_1186_s40035_023_00345_1 crossref_primary_10_1080_14728222_2023_2199923 crossref_primary_10_1128_spectrum_00292_25 crossref_primary_10_1097_MD_0000000000008699 crossref_primary_10_1002_aorn_12073 crossref_primary_10_1515_tnsci_2022_0315 crossref_primary_10_3390_ijms21030880 crossref_primary_10_1007_s13760_021_01596_3 crossref_primary_10_1016_j_conb_2019_11_019 crossref_primary_10_1073_pnas_1917054116 crossref_primary_10_3389_fnins_2020_00145 crossref_primary_10_1002_acn3_378 crossref_primary_10_1136_practneurol_2018_001935 crossref_primary_10_1007_s00401_022_02453_0 crossref_primary_10_3390_biom10030469 crossref_primary_10_1038_s41598_024_56789_6 crossref_primary_10_1001_jamanetworkopen_2021_46319 crossref_primary_10_1038_s41598_017_15778_8 crossref_primary_10_3233_JAD_170770 crossref_primary_10_1007_s12035_018_0926_y crossref_primary_10_1212_WNL_0000000000214040 crossref_primary_10_1002_acn3_52230 crossref_primary_10_3390_diagnostics11050788 crossref_primary_10_1016_j_clinbiochem_2019_08_008 crossref_primary_10_1016_j_coph_2018_10_001 crossref_primary_10_1186_s40035_022_00283_4 crossref_primary_10_3389_fnagi_2022_872629 crossref_primary_10_7759_cureus_75887 crossref_primary_10_3389_fbioe_2020_570692 crossref_primary_10_1016_j_exger_2022_111842 crossref_primary_10_3389_fnbeh_2020_00055 crossref_primary_10_1016_j_neubiorev_2024_105997 crossref_primary_10_1186_s12883_023_03193_8 crossref_primary_10_1007_s12035_018_1007_y crossref_primary_10_1016_j_cll_2020_04_002 crossref_primary_10_1016_j_neuint_2022_105306 crossref_primary_10_1007_s13760_018_0995_8 crossref_primary_10_1038_s41598_019_42449_7 crossref_primary_10_20517_and_2025_18 crossref_primary_10_4081_ejh_2021_3298 crossref_primary_10_1080_14737159_2017_1368389 crossref_primary_10_3390_v13050789 crossref_primary_10_1038_s41374_019_0282_1 crossref_primary_10_1080_14737175_2021_1965879 crossref_primary_10_3233_ADR_230173 crossref_primary_10_1038_s41531_023_00519_8 crossref_primary_10_1111_cxo_12604 crossref_primary_10_3390_pathogens12020309 crossref_primary_10_3389_fbioe_2020_585896 crossref_primary_10_1016_S1474_4422_20_30477_4 crossref_primary_10_1038_s41598_017_10922_w crossref_primary_10_1016_j_banm_2022_04_002 crossref_primary_10_1159_000486712 crossref_primary_10_1186_s40478_020_0887_z crossref_primary_10_3389_fnagi_2022_853050 crossref_primary_10_1007_s11940_017_0474_1 crossref_primary_10_1128_AAC_01441_17 crossref_primary_10_3389_fneur_2018_00242 crossref_primary_10_1097_01_NT_0000615632_73773_71 crossref_primary_10_1093_brain_awad101 crossref_primary_10_1136_bcr_2019_229729 crossref_primary_10_1007_s00401_018_1947_3 crossref_primary_10_1002_acn3_51219 crossref_primary_10_3389_fnins_2022_902077 crossref_primary_10_1128_JVI_00926_17 crossref_primary_10_1186_s12985_020_01309_x crossref_primary_10_1002_acn3_51057 crossref_primary_10_1002_alz_14422 crossref_primary_10_1097_MD_0000000000010162 crossref_primary_10_1016_j_coph_2019_04_019 crossref_primary_10_1186_s13073_018_0536_3 crossref_primary_10_3390_ijms231810210 crossref_primary_10_1136_bcr_2018_229018 crossref_primary_10_1186_s40035_022_00311_3 crossref_primary_10_3390_diagnostics11101821 crossref_primary_10_1002_ana_25579 crossref_primary_10_1186_s12877_023_04556_z |
| ContentType | Journal Article |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1001/jamaneurol.2016.4614 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Medicine |
| EISSN | 2168-6157 |
| ExternalDocumentID | 27942718 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GroupedDBID | 0R~ 4.4 53G AAGZG ABIVO ABJNI ACDNT ACGFS ACPRK ADBBV AENEX AFRAH AHMBA ALMA_UNASSIGNED_HOLDINGS AMJDE ANMPU BRYMA C45 CGR CUY CVF EBD EBS ECM EIF EJD EMOBN EX3 H13 NPM OB2 OBH OHH OVD PQQKQ RAJ SV3 TEORI WOW 7X8 ADHGD |
| ID | FETCH-LOGICAL-a471t-3bc397416a70fc7abd35deb8fff3380e2d2de300559ec1538cea193fa9aaba112 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 145 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000395663600010&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 2168-6157 |
| IngestDate | Sun Nov 09 09:55:03 EST 2025 Thu Jan 02 23:09:59 EST 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 2 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-a471t-3bc397416a70fc7abd35deb8fff3380e2d2de300559ec1538cea193fa9aaba112 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | http://hdl.handle.net/11380/1279602 |
| PMID | 27942718 |
| PQID | 1852664906 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_1852664906 pubmed_primary_27942718 |
| PublicationCentury | 2000 |
| PublicationDate | 2017-02-01 |
| PublicationDateYYYYMMDD | 2017-02-01 |
| PublicationDate_xml | – month: 02 year: 2017 text: 2017-02-01 day: 01 |
| PublicationDecade | 2010 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | JAMA neurology |
| PublicationTitleAlternate | JAMA Neurol |
| PublicationYear | 2017 |
| References | 27942712 - JAMA Neurol. 2017 Feb 1;74(2):144-145 |
| References_xml | – reference: 27942712 - JAMA Neurol. 2017 Feb 1;74(2):144-145 |
| SSID | ssj0000800434 |
| Score | 2.5892346 |
| Snippet | Early and accurate in vivo diagnosis of Creutzfeldt-Jakob disease (CJD) is necessary for quickly distinguishing treatable from untreatable rapidly progressive... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 155 |
| SubjectTerms | Aged Algorithms Case-Control Studies Creutzfeldt-Jakob Syndrome - cerebrospinal fluid Creutzfeldt-Jakob Syndrome - diagnosis Creutzfeldt-Jakob Syndrome - pathology Female Humans Italy Male Middle Aged Olfactory Mucosa - metabolism Olfactory Mucosa - pathology Prions - cerebrospinal fluid Sensitivity and Specificity |
| Title | Diagnosis of Human Prion Disease Using Real-Time Quaking-Induced Conversion Testing of Olfactory Mucosa and Cerebrospinal Fluid Samples |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/27942718 https://www.proquest.com/docview/1852664906 |
| Volume | 74 |
| WOSCitedRecordID | wos000395663600010&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LSwMxEA5qRbz4ftQXEbxGt_vMnkSqxUtr1Qq9Ldk8oFB2a7cV_AX-bWeSbT0JgpdlD9kQktmZbx6Zj5CrVGltAsUZB1vJwN-Q8CYFS6XHQxMBQs49SzaR9Hp8OEz7dcCtqssqFzrRKmpVSoyR3-Al3zgOUy--nbwzZI3C7GpNobFKGgFAGZTqZMiXMRZEQ6FNLPutmIOXFCXL23Ou8ZBtGokZiFZ8HcZ4lec3nGntTWf7vyvdIVs10qR3TjR2yYou9shGt86l75Ove1dlN6poaagN5tP-FI6J3rukDbXlBPQFoCTDmyL0eW6pqxjSfUitaBsr1m24jQ6wWQcMhpmexo7D55N2sRpeUFHAUD0F5xs5SnBNnfF8pOirwM7E1QF56zwM2o-spmVgAizZjAW5BBADQE4knpGJyFUQKZ1zYwz4u572la80dsGPUi1RoUotACYakQqRC8B3h2StKAt9TKgvhfJkJGQgFDiyMBc3njYwQZwnSoZNcrnY4gzEHnMZcGTlvMp-NrlJjtw5ZRPXnyPzQcf4YHNP_vD1Kdn00VDbOuwz0jDw0-tzsi4_ZqNqemHlCZ69fvcbxfTYdg |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Diagnosis+of+Human+Prion+Disease+Using+Real-Time+Quaking-Induced+Conversion+Testing+of+Olfactory+Mucosa+and+Cerebrospinal+Fluid+Samples&rft.jtitle=JAMA+neurology&rft.au=Bongianni%2C+Matilde&rft.au=Orr%C3%B9%2C+Christina&rft.au=Groveman%2C+Bradley+R&rft.au=Sacchetto%2C+Luca&rft.date=2017-02-01&rft.eissn=2168-6157&rft.volume=74&rft.issue=2&rft.spage=155&rft_id=info:doi/10.1001%2Fjamaneurol.2016.4614&rft_id=info%3Apmid%2F27942718&rft_id=info%3Apmid%2F27942718&rft.externalDocID=27942718 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2168-6157&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2168-6157&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2168-6157&client=summon |