A rapid and simple clonality assay for bovine leukemia virus-infected cells by amplified fragment length polymorphism (AFLP) analysis

Enzootic bovine leukosis (EBL) is routinely diagnosed based on external manifestations at the farm, such as the presence of tumors and/or general lymph node enlargement. However, due to the nonspecific clinical manifestations of EBL, over half of EBL cases are unrecognized at the farm, with most cas...

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Veröffentlicht in:Microbiology spectrum Jg. 13; H. 1; S. e0171424
Hauptverfasser: Kobayashi, Tomoko, Makimoto, Sakurako, Ohnuki, Nagaki, Hossain, Md Belal, Jahan, M. Ishrat, Matsuo, Misaki, Imakawa, Kazuhiko, Satou, Yorifumi
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States American Society for Microbiology 07.01.2025
Schlagworte:
AFLP
Amplified Fragment Length Polymorphism Analysis - methods
Animals
Bovine leukemia virus
Cattle
clonality
enzootic bovine leukosis
Enzootic Bovine Leukosis - diagnosis
Enzootic Bovine Leukosis - virology
Leukemia Virus, Bovine - genetics
Leukemia Virus, Bovine - isolation & purification
Methods and Protocols
Polymerase Chain Reaction - methods
retrovirus
Virology
retrovirus
clonality
enzootic bovine leukosis
Bovine leukemia virus
AFLP
ISSN:2165-0497, 2165-0497
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Beschreibung
Zusammenfassung:Enzootic bovine leukosis (EBL) is routinely diagnosed based on external manifestations at the farm, such as the presence of tumors and/or general lymph node enlargement. However, due to the nonspecific clinical manifestations of EBL, over half of EBL cases are unrecognized at the farm, with most cases being diagnosed during postmortem inspection at the slaughterhouse. Early detection and monitoring of clonal expansion are necessary for managing EBL and reducing economic losses. In this study, we developed BLV-AFLP that represents a significant advancement in the diagnosis of EBL in cattle. This method can rapidly assess the clonal proliferation of BLV-infected cells, crucial for distinguishing between asymptomatic and EBL cattle. Additionally, tracking clonal dynamics offers insights into the disease’s progression, potentially providing strategies for avoiding economic losses. Overall, as BLV-AFLP is a simple and rapid test for detecting EBL, it is feasible and efficient for routine veterinary practice.
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The authors declare no conflict of interest.
ISSN:2165-0497
2165-0497
DOI:10.1128/spectrum.01714-24