Molecular mechanisms controlling phosphate-induced downregulation of the yeast Pho84 phosphate transporter

In Saccharomyces cerevisiae, phosphate uptake is mainly dependent on the proton-coupled Pho84 permease under phosphate-limited growth conditions. Phosphate addition causes Pho84-mediated activation of the protein kinase A (PKA) pathway as well as rapid internalization and vacuolar breakdown of Pho84...

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Vydané v:Biochemistry (Easton) Ročník 48; číslo 21; s. 4497 - 4505
Hlavní autori: Lundh, Fredrik, Mouillon, Jean-Marie, Samyn, Dieter, Stadler, Kent, Popova, Yulia, Lagerstedt, Jens O, Thevelein, Johan M, Persson, Bengt L
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States 02.06.2009
Predmet:
Cyclic AMP-Dependent Protein Kinases - metabolism
Down-Regulation - drug effects
Feedback, Physiological
Intracellular Space - metabolism
Phosphates - metabolism
Phosphates - pharmacology
Phosphorylation - drug effects
Protein Transport - drug effects
Proton-Phosphate Symporters - chemistry
Proton-Phosphate Symporters - metabolism
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - chemistry
Saccharomyces cerevisiae Proteins - metabolism
Signal Transduction - drug effects
Ubiquitin - metabolism
Up-Regulation - drug effects
ISSN:1520-4995
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Shrnutí:In Saccharomyces cerevisiae, phosphate uptake is mainly dependent on the proton-coupled Pho84 permease under phosphate-limited growth conditions. Phosphate addition causes Pho84-mediated activation of the protein kinase A (PKA) pathway as well as rapid internalization and vacuolar breakdown of Pho84. We show that Pho84 undergoes phosphate-induced phosphorylation and subsequent ubiquitination on amino acids located in the large middle intracellular loop prior to endocytosis. The attachment of ubiquitin is dependent on the ubiquitin conjugating enzymes Ubc2 and Ubc4. In addition, we show that the Pho84 endocytotic process is delayed in strains with reduced PKA activity. Our results suggest that Pho84-mediated activation of the PKA pathway is responsible for its own downregulation by phosphorylation, ubiquination, internalization, and vacuolar breakdown.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1520-4995
DOI:10.1021/bi9001198