Green-to-Red Photoconvertible Dronpa Mutant for Multimodal Super-resolution Fluorescence Microscopy

Advanced imaging techniques crucially depend on the labels used. In this work, we present the structure-guided design of a fluorescent protein that displays both reversibly photochromic and green-to-red photoconversion behavior. We first designed ffDronpa, a mutant of the photochromic fluorescent pr...

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Bibliographic Details
Published in:	ACS nano Vol. 8; no. 2; pp. 1664 - 1673
Main Authors:	Moeyaert, Benjamien, Nguyen Bich, Ngan, De Zitter, Elke, Rocha, Susana, Clays, Koen, Mizuno, Hideaki, van Meervelt, Luc, Hofkens, Johan, Dedecker, Peter
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 25.02.2014
Subjects:	Biochemistry, Molecular Biology Biophysics Displays Fluctuation Fluorescence Imaging Life Sciences Microscopy Nanostructure Optimization Proteins Strategy Structural Biology fluorescent proteins protein engineering PALM Dronpa pcSOFI crystal structure determination super-resolution fluorescence microscopy
ISSN:	1936-0851, 1936-086X, 1936-086X
Online Access:	Get full text
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