Femtosecond UV studies of the electronic relaxation processes in Cytochrome c

We report on an experimental study with UV and visible ultrafast time-gated emission and transient absorption of the early photodynamics of horse heart Cytochrome c in both ferric and ferrous redox states. A clear separation in time and energy of tryptophan and haem emission is observed. Excitation...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:The journal of physical chemistry. B Ročník 115; číslo 46; s. 13723
Hlavní autoři: Bräm, Olivier, Consani, Cristina, Cannizzo, Andrea, Chergui, Majed
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 24.11.2011
Témata:
Animals
Cytochromes c - chemistry
Electrons
Energy Transfer
Heme - chemistry
Horses
Oxidation-Reduction
Spectrophotometry, Ultraviolet
Time Factors
Tryptophan - chemistry
ISSN:1520-5207, 1520-5207
On-line přístup:Zjistit podrobnosti o přístupu
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:We report on an experimental study with UV and visible ultrafast time-gated emission and transient absorption of the early photodynamics of horse heart Cytochrome c in both ferric and ferrous redox states. A clear separation in time and energy of tryptophan and haem emission is observed. Excitation of the haem via resonant energy transfer from the tryptophan residue is observed in the subsequent haem electronic relaxation. Different Trp-haem energy transfer time constants of the ferrous and ferric forms are obtained. An almost instantaneous relaxation to the lowest singlet excited state (corresponding to the so-called Q band) characterizes the earliest electronic dynamics of the haem, independent of excitation energy, while dark intermediate states govern the ground-state recovery. The information gathered in these two experiments and in the literature allows us to propose a simple scheme for the electronic relaxation leading to ligand dissociation.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1520-5207
1520-5207
DOI:10.1021/jp207615u