Self-Powered Temperature Sensor with Seebeck Effect Transduction for Photothermal-Thermoelectric Coupled Immunoassay

A self-powered temperature sensor based on Seebeck effect transduction was designed for photothermal-thermoelectric coupled immunoassay of α-fetoprotein (AFP). In this system, glucose oxidase (GOx)-conjugated detection antibody was first captured onto the microplate by target-induced sandwich-type i...

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Veröffentlicht in:Analytical chemistry (Washington) Jg. 92; H. 3; S. 2809
Hauptverfasser: Huang, Lingting, Chen, Jialun, Yu, Zhonghua, Tang, Dianping
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 04.02.2020
Schlagworte:
alpha-Fetoproteins - analysis
Biosensing Techniques - instrumentation
Electrochemical Techniques - instrumentation
Immunoassay - instrumentation
Photochemical Processes
Temperature
ISSN:1520-6882, 1520-6882
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Zusammenfassung:A self-powered temperature sensor based on Seebeck effect transduction was designed for photothermal-thermoelectric coupled immunoassay of α-fetoprotein (AFP). In this system, glucose oxidase (GOx)-conjugated detection antibody was first captured onto the microplate by target-induced sandwich-type immunoreaction. Thereafter, the as-generated hydrogen peroxide via the GOx-glucose system oxidized 3,3',5,5'-tetrametylbenzidine (TMB) into photothermal product oxidized TMB (ox-TMB). Under near-infrared (NIR) laser irradiation, the temperature change of ox-TMB was read out in an electrical signal by the flexible thermoelectric module in a 3D-printed integrated detection device. Under optimal conditions, the photothermal-thermoelectric coupled immunoassay exhibited a limit of detection of 0.39 ng mL AFP over a dynamic linear range from 0.5 to 60 ng mL . Impressively, such a strategy presented herein offers tremendous potentials for applying many other high-efficiency thermoelectric materials in ultrasensitive biosensors.
Bibliographie:ObjectType-Article-1
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ISSN:1520-6882
1520-6882
DOI:10.1021/acs.analchem.9b05218