Endothelial cell culture model for replication of physiological profiles of pressure, flow, stretch, and shear stress in vitro
The phenotype and function of vascular cells in vivo are influenced by complex mechanical signals generated by pulsatile hemodynamic loading. Physiologically relevant in vitro studies of vascular cells therefore require realistic environments where in vivo mechanical loading conditions can be accura...
Uloženo v:
| Vydáno v: | Analytical chemistry (Washington) Ročník 83; číslo 8; s. 3170 |
|---|---|
| Hlavní autoři: | , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
| Vydáno: |
United States
15.04.2011
|
| Témata: | |
| ISSN: | 1520-6882, 1520-6882 |
| On-line přístup: | Zjistit podrobnosti o přístupu |
| Tagy: |
Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
|
| Abstract | The phenotype and function of vascular cells in vivo are influenced by complex mechanical signals generated by pulsatile hemodynamic loading. Physiologically relevant in vitro studies of vascular cells therefore require realistic environments where in vivo mechanical loading conditions can be accurately reproduced. To accomplish a realistic in vivo-like loading environment, we designed and fabricated an Endothelial Cell Culture Model (ECCM) to generate physiological pressure, stretch, and shear stress profiles associated with normal and pathological cardiac flow states. Cells within this system were cultured on a stretchable, thin (∼500 μm) planar membrane within a rectangular flow channel and subject to constant fluid flow. Under pressure, the thin planar membrane assumed a concave shape, representing a segment of the blood vessel wall. Pulsatility was introduced using a programmable pneumatically controlled collapsible chamber. Human aortic endothelial cells (HAECs) were cultured within this system under normal conditions and compared to HAECs cultured under static and "flow only" (13 dyn/cm(2)) control conditions using microscopy. Cells cultured within the ECCM were larger than both controls and assumed an ellipsoidal shape. In contrast to static control control cells, ECCM-cultured cells exhibited alignment of cytoskeletal actin filaments and high and continuous expression levels of β-catenin indicating an in vivo-like phenotype. In conclusion, design, fabrication, testing, and validation of the ECCM for culture of ECs under realistic pressure, flow, strain, and shear loading seen in normal and pathological conditions was accomplished. The ECCM therefore is an enabling technology that allows for study of ECs under physiologically relevant biomechanical loading conditions in vitro. |
|---|---|
| AbstractList | The phenotype and function of vascular cells in vivo are influenced by complex mechanical signals generated by pulsatile hemodynamic loading. Physiologically relevant in vitro studies of vascular cells therefore require realistic environments where in vivo mechanical loading conditions can be accurately reproduced. To accomplish a realistic in vivo-like loading environment, we designed and fabricated an Endothelial Cell Culture Model (ECCM) to generate physiological pressure, stretch, and shear stress profiles associated with normal and pathological cardiac flow states. Cells within this system were cultured on a stretchable, thin (∼500 μm) planar membrane within a rectangular flow channel and subject to constant fluid flow. Under pressure, the thin planar membrane assumed a concave shape, representing a segment of the blood vessel wall. Pulsatility was introduced using a programmable pneumatically controlled collapsible chamber. Human aortic endothelial cells (HAECs) were cultured within this system under normal conditions and compared to HAECs cultured under static and "flow only" (13 dyn/cm(2)) control conditions using microscopy. Cells cultured within the ECCM were larger than both controls and assumed an ellipsoidal shape. In contrast to static control control cells, ECCM-cultured cells exhibited alignment of cytoskeletal actin filaments and high and continuous expression levels of β-catenin indicating an in vivo-like phenotype. In conclusion, design, fabrication, testing, and validation of the ECCM for culture of ECs under realistic pressure, flow, strain, and shear loading seen in normal and pathological conditions was accomplished. The ECCM therefore is an enabling technology that allows for study of ECs under physiologically relevant biomechanical loading conditions in vitro.The phenotype and function of vascular cells in vivo are influenced by complex mechanical signals generated by pulsatile hemodynamic loading. Physiologically relevant in vitro studies of vascular cells therefore require realistic environments where in vivo mechanical loading conditions can be accurately reproduced. To accomplish a realistic in vivo-like loading environment, we designed and fabricated an Endothelial Cell Culture Model (ECCM) to generate physiological pressure, stretch, and shear stress profiles associated with normal and pathological cardiac flow states. Cells within this system were cultured on a stretchable, thin (∼500 μm) planar membrane within a rectangular flow channel and subject to constant fluid flow. Under pressure, the thin planar membrane assumed a concave shape, representing a segment of the blood vessel wall. Pulsatility was introduced using a programmable pneumatically controlled collapsible chamber. Human aortic endothelial cells (HAECs) were cultured within this system under normal conditions and compared to HAECs cultured under static and "flow only" (13 dyn/cm(2)) control conditions using microscopy. Cells cultured within the ECCM were larger than both controls and assumed an ellipsoidal shape. In contrast to static control control cells, ECCM-cultured cells exhibited alignment of cytoskeletal actin filaments and high and continuous expression levels of β-catenin indicating an in vivo-like phenotype. In conclusion, design, fabrication, testing, and validation of the ECCM for culture of ECs under realistic pressure, flow, strain, and shear loading seen in normal and pathological conditions was accomplished. The ECCM therefore is an enabling technology that allows for study of ECs under physiologically relevant biomechanical loading conditions in vitro. The phenotype and function of vascular cells in vivo are influenced by complex mechanical signals generated by pulsatile hemodynamic loading. Physiologically relevant in vitro studies of vascular cells therefore require realistic environments where in vivo mechanical loading conditions can be accurately reproduced. To accomplish a realistic in vivo-like loading environment, we designed and fabricated an Endothelial Cell Culture Model (ECCM) to generate physiological pressure, stretch, and shear stress profiles associated with normal and pathological cardiac flow states. Cells within this system were cultured on a stretchable, thin (∼500 μm) planar membrane within a rectangular flow channel and subject to constant fluid flow. Under pressure, the thin planar membrane assumed a concave shape, representing a segment of the blood vessel wall. Pulsatility was introduced using a programmable pneumatically controlled collapsible chamber. Human aortic endothelial cells (HAECs) were cultured within this system under normal conditions and compared to HAECs cultured under static and "flow only" (13 dyn/cm(2)) control conditions using microscopy. Cells cultured within the ECCM were larger than both controls and assumed an ellipsoidal shape. In contrast to static control control cells, ECCM-cultured cells exhibited alignment of cytoskeletal actin filaments and high and continuous expression levels of β-catenin indicating an in vivo-like phenotype. In conclusion, design, fabrication, testing, and validation of the ECCM for culture of ECs under realistic pressure, flow, strain, and shear loading seen in normal and pathological conditions was accomplished. The ECCM therefore is an enabling technology that allows for study of ECs under physiologically relevant biomechanical loading conditions in vitro. |
| Author | Giridharan, Guruprasad A Nguyen, Mai-Dung Prabhu, Sumanth D Estrada, Rosendo Shakeri, Mostafa Parichehreh, Vahidreza Roussel, Thomas J Sethu, Palaniappan |
| Author_xml | – sequence: 1 givenname: Rosendo surname: Estrada fullname: Estrada, Rosendo organization: Department of Bioengineering, Speed School of Engineering, University of Louisville, Louisville, Kentucky 40208, USA – sequence: 2 givenname: Guruprasad A surname: Giridharan fullname: Giridharan, Guruprasad A – sequence: 3 givenname: Mai-Dung surname: Nguyen fullname: Nguyen, Mai-Dung – sequence: 4 givenname: Thomas J surname: Roussel fullname: Roussel, Thomas J – sequence: 5 givenname: Mostafa surname: Shakeri fullname: Shakeri, Mostafa – sequence: 6 givenname: Vahidreza surname: Parichehreh fullname: Parichehreh, Vahidreza – sequence: 7 givenname: Sumanth D surname: Prabhu fullname: Prabhu, Sumanth D – sequence: 8 givenname: Palaniappan surname: Sethu fullname: Sethu, Palaniappan |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/21413699$$D View this record in MEDLINE/PubMed |
| BookMark | eNpNUMtOwzAQtFARfcCBH0C-cWnA3qROckRVeUiVuMA5cuM1MXLiYDugXvh2QgGJy85qZne1M3My6VyHhJxzdsUZ8GtZA2NQlsURmfEVsEQUBUz-9VMyD-GVMc4ZFydkCjzjqSjLGfncdMrFBq2RltZoxzLYOHikrVNoqXaeeuytqWU0rqNO077ZB-Osexk5S3vvtLEYDorHEMbdJdXWfSxpiB5j3Syp7BQNDUp_oEKgpqPvJnp3So61tAHPfnFBnm83T-v7ZPt497C-2SYyS_OYaC7EDlLQkNZ5AVJptqpLhpnkCmVRc-ApClVolYs6LbOUqzGPjEupMq0hgwW5_Lk7vvs2YIhVa8K3XdmhG0JVCIC8hBEW5OJ3cti1qKrem1b6ffUXGXwBPc1wqA |
| CitedBy_id | crossref_primary_10_1016_j_bbe_2024_08_001 crossref_primary_10_1002_btpr_1627 crossref_primary_10_1186_s12938_016_0273_z crossref_primary_10_1016_j_cbpa_2012_03_014 crossref_primary_10_1002_admt_202201778 crossref_primary_10_1016_j_mtbio_2025_101599 crossref_primary_10_1186_s12915_024_02009_6 crossref_primary_10_1002_mabi_202200466 crossref_primary_10_1021_acsbiomaterials_8b00628 crossref_primary_10_1063_1_3608137 crossref_primary_10_3390_mi12020139 crossref_primary_10_1038_ncomms8301 crossref_primary_10_1146_annurev_fluid_010816_060246 crossref_primary_10_1002_elps_201600429 crossref_primary_10_1016_j_healun_2013_02_010 crossref_primary_10_1371_journal_pone_0142945 crossref_primary_10_1371_journal_pone_0061275 crossref_primary_10_3389_fphys_2021_733464 crossref_primary_10_1016_j_addr_2013_08_009 crossref_primary_10_1002_adhm_202100508 crossref_primary_10_32604_biocell_2021_014900 crossref_primary_10_3390_bios8030076 crossref_primary_10_1016_j_cmpb_2023_107926 crossref_primary_10_3389_fbioe_2021_791116 crossref_primary_10_3390_s24185872 crossref_primary_10_1097_MAT_0000000000001886 crossref_primary_10_1016_j_talanta_2022_123933 crossref_primary_10_1016_j_lfs_2018_09_026 crossref_primary_10_1016_j_addr_2024_115216 crossref_primary_10_1088_1748_605X_ac91ba crossref_primary_10_3390_antiox12010031 crossref_primary_10_1007_s00421_022_05041_y crossref_primary_10_1088_0960_1317_26_3_035013 crossref_primary_10_1002_biot_201300016 crossref_primary_10_3389_fphys_2021_705256 crossref_primary_10_1038_srep04951 crossref_primary_10_3390_mi10110790 crossref_primary_10_1016_j_jbiomech_2012_09_031 crossref_primary_10_1007_s13239_018_00394_y crossref_primary_10_1016_j_aca_2022_340456 crossref_primary_10_1002_elps_201300205 crossref_primary_10_1002_elps_202100128 crossref_primary_10_1038_srep38861 crossref_primary_10_1007_s10529_017_2354_x crossref_primary_10_1039_C3IB40267C crossref_primary_10_1063_1_4893272 crossref_primary_10_1007_s13239_012_0096_4 crossref_primary_10_1016_j_actbio_2021_02_031 crossref_primary_10_1007_s12195_016_0473_4 crossref_primary_10_1007_s12551_021_00815_8 crossref_primary_10_3390_ijms21218156 crossref_primary_10_1007_s10404_013_1275_9 crossref_primary_10_3390_app11199140 |
| ContentType | Journal Article |
| Copyright | 2011 American Chemical Society |
| Copyright_xml | – notice: 2011 American Chemical Society |
| DBID | CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1021/ac2002998 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Engineering Chemistry |
| EISSN | 1520-6882 |
| ExternalDocumentID | 21413699 |
| Genre | Research Support, U.S. Gov't, Non-P.H.S Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural |
| GrantInformation_xml | – fundername: NHLBI NIH HHS grantid: R01 HL-99014 |
| GroupedDBID | --- -DZ -~X .DC .K2 23M 4.4 53G 55A 5GY 5RE 5VS 6J9 7~N 85S AABXI AAHBH ABHFT ABHMW ABJNI ABMVS ABOCM ABPPZ ABQRX ABUCX ACBEA ACGFO ACGFS ACGOD ACIWK ACJ ACKOT ACNCT ACPRK ACS ADHLV AEESW AENEX AFEFF AFRAH AGXLV AHGAQ ALMA_UNASSIGNED_HOLDINGS AQSVZ BAANH BKOMP CGR CS3 CUPRZ CUY CVF D0L EBS ECM ED~ EIF EJD F5P GGK GNL IH9 IHE JG~ KZ1 LG6 LMP NPM P2P PQQKQ ROL RXW TAE TN5 UHB UI2 UKR VF5 VG9 W1F WH7 X6Y XSW YZZ ZCA ~02 7X8 ABBLG ABLBI |
| ID | FETCH-LOGICAL-a437t-f166b232f23c782adf05c90e4a1dea8c1213e6d8fd76c39431d02941aad4ff242 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 86 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000289223700046&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1520-6882 |
| IngestDate | Wed Oct 01 14:41:04 EDT 2025 Thu Apr 03 07:00:46 EDT 2025 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 8 |
| Language | English |
| License | 2011 American Chemical Society |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-a437t-f166b232f23c782adf05c90e4a1dea8c1213e6d8fd76c39431d02941aad4ff242 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 21413699 |
| PQID | 862279286 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_862279286 pubmed_primary_21413699 |
| PublicationCentury | 2000 |
| PublicationDate | 2011-04-15 |
| PublicationDateYYYYMMDD | 2011-04-15 |
| PublicationDate_xml | – month: 04 year: 2011 text: 2011-04-15 day: 15 |
| PublicationDecade | 2010 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Analytical chemistry (Washington) |
| PublicationTitleAlternate | Anal Chem |
| PublicationYear | 2011 |
| SSID | ssj0011016 |
| Score | 2.3346155 |
| Snippet | The phenotype and function of vascular cells in vivo are influenced by complex mechanical signals generated by pulsatile hemodynamic loading. Physiologically... |
| SourceID | proquest pubmed |
| SourceType | Aggregation Database Index Database |
| StartPage | 3170 |
| SubjectTerms | Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Cells, Cultured Endothelial Cells - cytology Humans Models, Biological Pressure Stress, Physiological |
| Title | Endothelial cell culture model for replication of physiological profiles of pressure, flow, stretch, and shear stress in vitro |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/21413699 https://www.proquest.com/docview/862279286 |
| Volume | 83 |
| WOSCitedRecordID | wos000289223700046&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV07T8MwELaAIgEDj_IqL93AWKtN4ibxhFDVioWqA0jdIju2pUpVUpJSNn47ZycpLIiBDFkiS9H5fP7uzv4-Qu55aGIZGUkVl5qySAjKcRVRiU9gDPp1XzqxiWgyiWczPq3P5pT1scomJrpArfLU1sh7iLwt110cPizfqBWNss3VWkFjm7QCRDL2RFc0-24i2MTU0aXaDAmRZEMs5Hs9S0zYx0gc_w4s3QYzPvrnrx2TwxpZwmPlCidkS2dtsjdsBN3a5OAH9-Ap-Rxlyt6-WqADgi3fQ0XCocGJ4wCCWSj0prsNuQFXBWmCJdRq36X74pL2QnfBLPKPLtgbKOgOXRCZgtKKZkN1KQXmGaznqyI_I6_j0cvwidZiDFSwIFpR44WhRPhl_CBFVCGU6Q9S3tdMeEqLOLXUcDpUsVFRmAYccYlCAzNPCMWMQSBwTnayPNOXBBBUIizRfoBTxthASCVDibFFe5rxVPsdAo2dEzSRNYHIdP5eJhtLd8hFNVfJsiLlSHwPt-OQ86u_B1-T_aoyzKg3uCEtgwtd35LddL2al8WdcyJ8T6bPX7Da1WY |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Endothelial+cell+culture+model+for+replication+of+physiological+profiles+of+pressure%2C+flow%2C+stretch%2C+and+shear+stress+in+vitro&rft.jtitle=Analytical+chemistry+%28Washington%29&rft.au=Estrada%2C+Rosendo&rft.au=Giridharan%2C+Guruprasad+A&rft.au=Nguyen%2C+Mai-Dung&rft.au=Roussel%2C+Thomas+J&rft.date=2011-04-15&rft.eissn=1520-6882&rft.volume=83&rft.issue=8&rft.spage=3170&rft_id=info:doi/10.1021%2Fac2002998&rft_id=info%3Apmid%2F21413699&rft_id=info%3Apmid%2F21413699&rft.externalDocID=21413699 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1520-6882&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1520-6882&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1520-6882&client=summon |