Fast DNA Sequencing via Transverse Electronic Transport

A rapid and low-cost method to sequence DNA would usher in a revolution in medicine. We propose and theoretically show the feasibility of a protocol for sequencing based on the distributions of transverse electrical currents of single-stranded DNA while it translocates through a nanopore. Our estima...

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Veröffentlicht in:Nano letters Jg. 6; H. 4; S. 779 - 782
Hauptverfasser: Lagerqvist, Johan, Zwolak, Michael, Di Ventra, Massimiliano
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Washington, DC American Chemical Society 01.04.2006
Schlagworte:
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Computer Simulation
Computer-Aided Design
Conformational dynamics in molecular biology
DNA - analysis
DNA - chemistry
DNA - radiation effects
Electrochemistry - instrumentation
Electrochemistry - methods
Electrodes
Electron Transport
Feasibility Studies
Fundamental and applied biological sciences. Psychology
Models, Chemical
Molecular biophysics
Oligonucleotide Array Sequence Analysis - instrumentation
Oligonucleotide Array Sequence Analysis - methods
Sequence Analysis, DNA - instrumentation
Sequence Analysis, DNA - methods
Human
Nucleotide sequence
DNA
DNA sequence
Nanopore
Genome
Implementation
Nanoporosity
ISSN:1530-6984, 1530-6992
Online-Zugang:Volltext
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Beschreibung
Zusammenfassung:A rapid and low-cost method to sequence DNA would usher in a revolution in medicine. We propose and theoretically show the feasibility of a protocol for sequencing based on the distributions of transverse electrical currents of single-stranded DNA while it translocates through a nanopore. Our estimates, based on the statistics of these distributions, reveal that sequencing of an entire human genome could be done with very high accuracy in a matter of hours without parallelization, that is, orders of magnitude faster than present techniques. The practical implementation of our approach would represent a substantial advancement in our ability to study, predict, and cure diseases from the perspective of the genetic makeup of each individual.
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ISSN:1530-6984
1530-6992
DOI:10.1021/nl0601076