Live Microscopy of Multicellular Spheroids with the Multimodal Near-Infrared Nanoparticles Reveals Differences in Oxygenation Gradients

Assessment of hypoxia, nutrients, metabolite gradients, and other hallmarks of the tumor microenvironment within 3D multicellular spheroid and organoid models represents a challenging analytical task. Here, we report red/near-infrared (NIR) emitting cell staining with O2-sensitive nanoparticles, whi...

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Bibliographic Details
Published in:	ACS nano Vol. 18; no. 19; pp. 12168 - 12186
Main Authors:	Debruyne, Angela C., Okkelman, Irina A., Heymans, Nina, Pinheiro, Cláudio, Hendrix, An, Nobis, Max, Borisov, Sergey M., Dmitriev, Ruslan I.
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 14.05.2024
Subjects:	Humans Infrared Rays Metalloporphyrins - chemistry Metalloporphyrins - pharmacology Microscopy, Fluorescence Nanoparticles - chemistry Oxygen - chemistry Oxygen - metabolism Spheroids, Cellular - drug effects Spheroids, Cellular - metabolism hypoxia nanoparticles cancer FLIM oxygenation fluorescence microscopy multicellular spheroids
ISSN:	1936-0851, 1936-086X, 1936-086X
Online Access:	Get full text
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