Redondovirus Diversity and Evolution on Global, Individual, and Molecular Scales

is a newly established family of circular Rep-encoding single-stranded (CRESS) DNA viruses found in the human ororespiratory tract. Redondoviruses were previously found in ∼15% of respiratory specimens from U.S. urban subjects; levels were elevated in individuals with periodontitis or critical illne...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Journal of virology Ročník 95; číslo 21; s. e0081721
Hlavní autoři: Taylor, Louis J, Dothard, Marisol I, Rubel, Meagan A, Allen, Audrey A, Hwang, Young, Roche, Aoife M, Graham-Wooten, Jevon, Fitzgerald, Ayannah S, Khatib, Layla A, Ranciaro, Alessia, Thompson, Simon R, Beggs, William R, Campbell, Michael C, Mokone, Gaonyadiwe G, Mpoloka, Sununguko Wata, Fokunang, Charles, Njamnshi, Alfred K, Mbunwe, Eric, Woldemeskel, Dawit, Belay, Gurja, Nyambo, Thomas, Tishkoff, Sarah A, Collman, Ronald G, Bushman, Frederic D
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 13.10.2021
Témata:
Africa - epidemiology
Biodiversity
Critical Illness
DNA Virus Infections - epidemiology
DNA Virus Infections - virology
DNA Viruses - classification
DNA Viruses - genetics
DNA Viruses - metabolism
DNA-Binding Proteins - metabolism
Evolution, Molecular
Genome, Viral
Humans
Metagenomics
Mouth - virology
Periodontitis - virology
Phylogeny
Prevalence
Respiratory System - virology
Rural Population
Saliva - virology
United States - epidemiology
Viral Proteins - metabolism
United States
Africa
vientovirus
genetic recombination
brisavirus
CRESS viruses
Redondoviridae
evolution
rep protein
redondovirus
ISSN:1098-5514, 1098-5514
On-line přístup:Zjistit podrobnosti o přístupu
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:is a newly established family of circular Rep-encoding single-stranded (CRESS) DNA viruses found in the human ororespiratory tract. Redondoviruses were previously found in ∼15% of respiratory specimens from U.S. urban subjects; levels were elevated in individuals with periodontitis or critical illness. Here, we report higher redondovirus prevalence in saliva samples: four rural African populations showed 61 to 82% prevalence, and an urban U.S. population showed 32% prevalence. Longitudinal, limiting-dilution single-genome sequencing revealed diverse strains of both redondovirus species ( and ) in single individuals, persistence over time, and evidence of intergenomic recombination. Computational analysis of viral genomes identified a recombination hot spot associated with a conserved potential DNA stem-loop structure. To assess the possible role of this site in recombination, we carried out studies which showed that this potential stem-loop was cleaved by the virus-encoded Rep protein. In addition, in reconstructed reactions, a Rep-DNA covalent intermediate was shown to mediate DNA strand transfer at this site. Thus, redondoviruses are highly prevalent in humans, found in individuals on multiple continents, heterogeneous even within individuals and encode a Rep protein implicated in facilitating recombination. is a recently established family of DNA viruses predominantly found in the human respiratory tract and associated with multiple clinical conditions. In this study, we found high redondovirus prevalence in saliva from urban North American individuals and nonindustrialized African populations in Botswana, Cameroon, Ethiopia, and Tanzania. Individuals on both continents harbored both known redondovirus species. Global prevalence of both species suggests that redondoviruses have long been associated with humans but have remained undetected until recently due to their divergent genomes. By sequencing single redondovirus genomes in longitudinally sampled humans, we found that redondoviruses persisted over time within subjects and likely evolve by recombination. The Rep protein encoded by redondoviruses catalyzes multiple reactions , consistent with a role in mediating DNA replication and recombination. In summary, we identify high redondovirus prevalence in humans across multiple continents, longitudinal heterogeneity and persistence, and potential mechanisms of redondovirus evolution by recombination.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1098-5514
1098-5514
DOI:10.1128/JVI.00817-21