Effects of Microplate Type and Broth Additives on Microdilution MIC Susceptibility Assays

The determination of antibiotic potency against bacterial strains by assessment of their minimum inhibitory concentration normally uses a standardized broth microdilution assay procedure developed more than 50 years ago. However, certain antibiotics require modified assay conditions in order to obse...

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Vydáno v:	Antimicrobial agents and chemotherapy Ročník 63; číslo 1
Hlavní autoři:	Kavanagh, Angela, Ramu, Soumya, Gong, Yujing, Cooper, Matthew A, Blaskovich, Mark A T
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States 01.01.2019
Témata:	Aminoglycosides - chemistry Aminoglycosides - pharmacology Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Calcium - pharmacology Ciprofloxacin - chemistry Ciprofloxacin - pharmacology Colistin - chemistry Colistin - pharmacology Culture Media - chemistry Culture Media - pharmacology Depsipeptides - chemistry Depsipeptides - pharmacology Escherichia coli - drug effects Escherichia coli - growth & development Escherichia coli - metabolism Factor Analysis, Statistical Lipoglycopeptides - chemistry Lipoglycopeptides - pharmacology Methicillin-Resistant Staphylococcus aureus - drug effects Methicillin-Resistant Staphylococcus aureus - growth & development Methicillin-Resistant Staphylococcus aureus - metabolism Microbial Sensitivity Tests - instrumentation Oxacillin - chemistry Oxacillin - pharmacology Penicillin G - chemistry Penicillin G - pharmacology Plastics - chemistry Polymyxin B - chemistry Polymyxin B - pharmacology Polysorbates - pharmacology Rifampin - chemistry Rifampin - pharmacology Teicoplanin - analogs & derivatives Teicoplanin - chemistry Teicoplanin - pharmacology Trimethoprim - chemistry Trimethoprim - pharmacology Vancomycin - chemistry Vancomycin - pharmacology broth microdilution assay additive effects microtiter plate effects MIC
ISSN:	1098-6596, 1098-6596
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Shrnutí:	The determination of antibiotic potency against bacterial strains by assessment of their minimum inhibitory concentration normally uses a standardized broth microdilution assay procedure developed more than 50 years ago. However, certain antibiotics require modified assay conditions in order to observe optimal activity. For example, daptomycin requires medium supplemented with Ca , and the lipoglycopeptides dalbavancin and oritavancin require Tween 80 to be added to the growth medium to prevent the depletion of free drug via adsorption to the plastic microplate. In this report, we examine systematically the effects of several different plate types on microdilution broth MIC values for a set of antibiotics against Gram-positive and Gram-negative bacteria, both in medium alone and in medium supplemented with the commonly used additives Tween 80, lysed horse blood, and 50% human serum. We observed very significant differences in measured MICs (up to 100-fold) for some lipophilic antibiotics, such as the Gram-positive lipoglycopeptide dalbavancin and the Gram-negative lipopeptide polymyxins, and found that nonspecific binding plates can replace the need for surfactant additives. Microtiter plate types and any additives should be specified when reporting broth dilution MIC values, as results can vary dramatically for some classes of antibiotics.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1098-6596 1098-6596
DOI:	10.1128/AAC.01760-18