Metal–Organic Framework Encapsulated Whole-Cell Vaccines Enhance Humoral Immunity against Bacterial Infection

The increasing rate of resistance of bacterial infection against antibiotics requires next generation approaches to fight potential pandemic spread. The development of vaccines against pathogenic bacteria has been difficult owing, in part, to the genetic diversity of bacteria. Hence, there are many...

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Vydáno v:ACS nano Ročník 15; číslo 11; s. 17426 - 17438
Hlavní autoři: Luzuriaga, Michael A, Herbert, Fabian C, Brohlin, Olivia R, Gadhvi, Jashkaran, Howlett, Thomas, Shahrivarkevishahi, Arezoo, Wijesundara, Yalini H, Venkitapathi, Sundharamani, Veera, Kavya, Ehrman, Ryanne, Benjamin, Candace E, Popal, Sarah, Burton, Michael D, Ingersoll, Molly A, De Nisco, Nicole J, Gassensmith, Jeremiah J
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States American Chemical Society 23.11.2021
Témata:
Animals
Antigens
Bacterial Infections
Escherichia coli
Immunity, Humoral
Immunology
Life Sciences
Metal-Organic Frameworks
Mice
Vaccination - methods
Vaccines
whole cell vaccine
vaccine
germinal center
MOF
E. coli
urinary tract infection
ISSN:1936-0851, 1936-086X, 1936-086X
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Shrnutí:The increasing rate of resistance of bacterial infection against antibiotics requires next generation approaches to fight potential pandemic spread. The development of vaccines against pathogenic bacteria has been difficult owing, in part, to the genetic diversity of bacteria. Hence, there are many potential target antigens and little a priori knowledge of which antigen/s will elicit protective immunity. The painstaking process of selecting appropriate antigens could be avoided with whole-cell bacteria; however, whole-cell formulations typically fail to produce long-term and durable immune responses. These complications are one reason why no vaccine against any type of pathogenic E. coli has been successfully clinically translated. As a proof of principle, we demonstrate a method to enhance the immunogenicity of a model pathogenic E. coli strain by forming a slow releasing depot. The E. coli strain CFT073 was biomimetically mineralized within a metal–organic framework (MOF). This process encapsulates the bacteria within 30 min in water and at ambient temperatures. Vaccination with this formulation substantially enhances antibody production and results in significantly enhanced survival in a mouse model of bacteremia compared to standard inactivated formulations.
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ISSN:1936-0851
1936-086X
1936-086X
DOI:10.1021/acsnano.1c03092