In vitro activity of antibiotic monotherapy and combination therapy with bacteriophages against Staphylococcus aureus LVAD-driveline infections

Current treatment strategies for S. aureus LVAD-driveline infections are based on in vitro antibiotic susceptibility of planktonic bacteria. However, LVAD infections are most often biofilm-related, which decreases antibiotic susceptibility significantly, resulting in discrepancies between in vitro a...

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Veröffentlicht in:Journal of clinical microbiology Jg. 63; H. 11; S. e0027225
Hauptverfasser: Molendijk, Michèle M., Verkaik, Nelianne J., de Vogel, Corné P., Lemmens-den Toom, Nicole, Knight, Gwenan M., Caliskan, Kadir, Bode, Lonneke G. M., Verbon, Annelies, Koopmans, Marion P. G., de Graaf, Miranda, van Wamel, Willem J. B.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States American Society for Microbiology 12.11.2025
Schlagworte:
Animal Models of Infection
Anti-Bacterial Agents - pharmacology
Antimicrobial Resistance
Bacterial Infections
Bacteriology
Bacteriophage-Pathogen Interactions
Bacteriophages
Bacteriophages - physiology
biofilm
Biofilm-Associated Resistance
Biofilms - drug effects
Biofilms - growth & development
Clinical Microbiology and Infectious Diseases
Heart-Assist Devices - adverse effects
Heart-Assist Devices - microbiology
Humans
isothermal microcalorimetry
LVAD driveline infection
Microbial Pathogenesis and Immunology
Microbial Sensitivity Tests
Outbreak Investigation in Healthcare Facilities
Phage Therapy
Phage Therapy - methods
Prosthesis-Related Infections - microbiology
Prosthesis-Related Infections - therapy
Rifampin - pharmacology
Staphylococcal Infections
Staphylococcal Infections - microbiology
Staphylococcal Infections - therapy
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - physiology
Staphylococcus aureus - virology
Staphylococcus Phages - physiology
Trichinellosis
Vaginal Fungal Infections
Virology
LVAD driveline infection
Staphylococcus aureus
bacteriophages
biofilm
isothermal microcalorimetry
ISSN:0095-1137, 1098-660X, 1098-660X
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Zusammenfassung:Current treatment strategies for S. aureus LVAD-driveline infections are based on in vitro antibiotic susceptibility of planktonic bacteria. However, LVAD infections are most often biofilm-related, which decreases antibiotic susceptibility significantly, resulting in discrepancies between in vitro antibiotic susceptibility and in vivo treatment success. Here, we have developed a novel in vitro assay to determine antibiotic susceptibility of S. aureus biofilm, grown in conditions relevant to LVAD-driveline infections. Next to antibiotic susceptibility, the susceptibility of this biofilm to bacteriophage mono- and combination treatment with antibiotics was evaluated as an alternative treatment strategy. In the future, this assay can be used to provide a better insight in in vivo antibiotic- and bacteriophage susceptibility of LVAD-driveline biofilms. Thereby improving in vivo treatment strategies for LVAD-driveline infections.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0095-1137
1098-660X
1098-660X
DOI:10.1128/jcm.00272-25