Antibody Profiling of Dengue Severities Using Flavivirus Protein Microarrays
Dengue is a viral disease transmitted by Aedes aegypti mosquitoes. According to the World Health Organization, about half of the world's population is at risk of dengue. There are four serotypes of the dengue virus. After infection with one serotype, it will be immune to such a serotype. Howeve...
Uložené v:
| Vydané v: | Analytical chemistry (Washington) Ročník 95; číslo 41; s. 15217 |
|---|---|
| Hlavní autori: | , , , , , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | English |
| Vydavateľské údaje: |
17.10.2023
|
| ISSN: | 1520-6882, 1520-6882 |
| On-line prístup: | Zistit podrobnosti o prístupe |
| Tagy: |
Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
|
| Abstract | Dengue is a viral disease transmitted by Aedes aegypti mosquitoes. According to the World Health Organization, about half of the world's population is at risk of dengue. There are four serotypes of the dengue virus. After infection with one serotype, it will be immune to such a serotype. However, subsequent infection with other serotypes will increase the risk of severe outcomes, e.g., dengue hemorrhagic fever, dengue shock syndrome, and even death. Since severe dengue is challenging to predict and lacks molecular markers, we aim to build a multiplexed Flavivirus protein microarray (Flaviarray) that includes all of the common Flaviviruses to profile the humoral immunity and cross-reactivity in the dengue patients with different outcomes. The Flaviarrays we fabricated contained 17 Flavivirus antigens with high reproducibility (R-square = 0.96) and low detection limits (172-214 pg). We collected serums from healthy subjects (n = 36) and dengue patients within 7 days after symptom onset (mild dengue (n = 21), hospitalized nonsevere dengue (n = 29), and severe dengue (n = 36)). After profiling the serum antibodies using Flaviarrays, we found that patients with severe dengue showed higher IgG levels against multiple Flavivirus antigens. With logistic regression, we found groups of markers with high performance in distinguishing dengue patients from healthy controls as well as hospitalized from mild cases (AUC > 0.9). We further reported some single markers that were suitable to separate dengue patients from healthy controls (AUC > 0.9) and hospitalized from mild outcomes (AUC > 0.8). Together, Flaviarray is a valuable tool to profile antibody specificities, uncover novel markers for decision-making, and shed some light on early preventions and treatments.Dengue is a viral disease transmitted by Aedes aegypti mosquitoes. According to the World Health Organization, about half of the world's population is at risk of dengue. There are four serotypes of the dengue virus. After infection with one serotype, it will be immune to such a serotype. However, subsequent infection with other serotypes will increase the risk of severe outcomes, e.g., dengue hemorrhagic fever, dengue shock syndrome, and even death. Since severe dengue is challenging to predict and lacks molecular markers, we aim to build a multiplexed Flavivirus protein microarray (Flaviarray) that includes all of the common Flaviviruses to profile the humoral immunity and cross-reactivity in the dengue patients with different outcomes. The Flaviarrays we fabricated contained 17 Flavivirus antigens with high reproducibility (R-square = 0.96) and low detection limits (172-214 pg). We collected serums from healthy subjects (n = 36) and dengue patients within 7 days after symptom onset (mild dengue (n = 21), hospitalized nonsevere dengue (n = 29), and severe dengue (n = 36)). After profiling the serum antibodies using Flaviarrays, we found that patients with severe dengue showed higher IgG levels against multiple Flavivirus antigens. With logistic regression, we found groups of markers with high performance in distinguishing dengue patients from healthy controls as well as hospitalized from mild cases (AUC > 0.9). We further reported some single markers that were suitable to separate dengue patients from healthy controls (AUC > 0.9) and hospitalized from mild outcomes (AUC > 0.8). Together, Flaviarray is a valuable tool to profile antibody specificities, uncover novel markers for decision-making, and shed some light on early preventions and treatments. |
|---|---|
| AbstractList | Dengue is a viral disease transmitted by Aedes aegypti mosquitoes. According to the World Health Organization, about half of the world's population is at risk of dengue. There are four serotypes of the dengue virus. After infection with one serotype, it will be immune to such a serotype. However, subsequent infection with other serotypes will increase the risk of severe outcomes, e.g., dengue hemorrhagic fever, dengue shock syndrome, and even death. Since severe dengue is challenging to predict and lacks molecular markers, we aim to build a multiplexed Flavivirus protein microarray (Flaviarray) that includes all of the common Flaviviruses to profile the humoral immunity and cross-reactivity in the dengue patients with different outcomes. The Flaviarrays we fabricated contained 17 Flavivirus antigens with high reproducibility (R-square = 0.96) and low detection limits (172-214 pg). We collected serums from healthy subjects (n = 36) and dengue patients within 7 days after symptom onset (mild dengue (n = 21), hospitalized nonsevere dengue (n = 29), and severe dengue (n = 36)). After profiling the serum antibodies using Flaviarrays, we found that patients with severe dengue showed higher IgG levels against multiple Flavivirus antigens. With logistic regression, we found groups of markers with high performance in distinguishing dengue patients from healthy controls as well as hospitalized from mild cases (AUC > 0.9). We further reported some single markers that were suitable to separate dengue patients from healthy controls (AUC > 0.9) and hospitalized from mild outcomes (AUC > 0.8). Together, Flaviarray is a valuable tool to profile antibody specificities, uncover novel markers for decision-making, and shed some light on early preventions and treatments.Dengue is a viral disease transmitted by Aedes aegypti mosquitoes. According to the World Health Organization, about half of the world's population is at risk of dengue. There are four serotypes of the dengue virus. After infection with one serotype, it will be immune to such a serotype. However, subsequent infection with other serotypes will increase the risk of severe outcomes, e.g., dengue hemorrhagic fever, dengue shock syndrome, and even death. Since severe dengue is challenging to predict and lacks molecular markers, we aim to build a multiplexed Flavivirus protein microarray (Flaviarray) that includes all of the common Flaviviruses to profile the humoral immunity and cross-reactivity in the dengue patients with different outcomes. The Flaviarrays we fabricated contained 17 Flavivirus antigens with high reproducibility (R-square = 0.96) and low detection limits (172-214 pg). We collected serums from healthy subjects (n = 36) and dengue patients within 7 days after symptom onset (mild dengue (n = 21), hospitalized nonsevere dengue (n = 29), and severe dengue (n = 36)). After profiling the serum antibodies using Flaviarrays, we found that patients with severe dengue showed higher IgG levels against multiple Flavivirus antigens. With logistic regression, we found groups of markers with high performance in distinguishing dengue patients from healthy controls as well as hospitalized from mild cases (AUC > 0.9). We further reported some single markers that were suitable to separate dengue patients from healthy controls (AUC > 0.9) and hospitalized from mild outcomes (AUC > 0.8). Together, Flaviarray is a valuable tool to profile antibody specificities, uncover novel markers for decision-making, and shed some light on early preventions and treatments. |
| Author | Lin, Wei-Hsun Du, Pin-Xian Lin, Pei-Chun Su, Wen-Yu Tsai, Pei-Shan Syu, Guan-Da Wan, Shu-Wen Lin, Ya-Lan Keskin, Batuhan Birol Lee, Nan-Yao Shih, Hsi-Chang Ho, Tzong-Shiann |
| Author_xml | – sequence: 1 givenname: Pei-Shan surname: Tsai fullname: Tsai, Pei-Shan – sequence: 2 givenname: Pin-Xian surname: Du fullname: Du, Pin-Xian – sequence: 3 givenname: Batuhan Birol surname: Keskin fullname: Keskin, Batuhan Birol – sequence: 4 givenname: Nan-Yao surname: Lee fullname: Lee, Nan-Yao – sequence: 5 givenname: Shu-Wen surname: Wan fullname: Wan, Shu-Wen – sequence: 6 givenname: Ya-Lan surname: Lin fullname: Lin, Ya-Lan – sequence: 7 givenname: Wen-Yu surname: Su fullname: Su, Wen-Yu – sequence: 8 givenname: Pei-Chun surname: Lin fullname: Lin, Pei-Chun – sequence: 9 givenname: Wei-Hsun surname: Lin fullname: Lin, Wei-Hsun – sequence: 10 givenname: Hsi-Chang surname: Shih fullname: Shih, Hsi-Chang – sequence: 11 givenname: Tzong-Shiann surname: Ho fullname: Ho, Tzong-Shiann – sequence: 12 givenname: Guan-Da surname: Syu fullname: Syu, Guan-Da |
| BookMark | eNpNT19LwzAcDDLBbfoNfMijL63506Tp45hOhYqC7nmk6S8z0iWatIV9ezv0wYfjjrvj4BZo5oMHhK4pySlh9FablGuvO_MBh5ybyZLiDM2pYCSTSrHZP32BFil9EkIpoXKO6pXvXRPaI36NwbrO-T0OFt-B3w-A32CE6HoHCW_TKdp0enSji0M69XtwHj87E4OOUR_TJTq3uktw9cdLtN3cv68fs_rl4Wm9qjPNlewzYLSgiqgJjeRVaw0pqRCGG-CEtFJI0zJVTT_ASgum4cxUTcPbEqyQrGBLdPO7-xXD9wCp3x1cMtB12kMY0o6psmBymi7YDzkJVsA |
| CitedBy_id | crossref_primary_10_1016_j_biotechadv_2024_108461 |
| ContentType | Journal Article |
| DBID | 7X8 |
| DOI | 10.1021/acs.analchem.3c02165 |
| DatabaseName | MEDLINE - Academic |
| DatabaseTitle | MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Engineering Chemistry |
| EISSN | 1520-6882 |
| GroupedDBID | --- -DZ -~X .DC .K2 23M 4.4 53G 55A 5GY 5RE 5VS 6J9 7X8 7~N 85S AABXI AAHBH ABBLG ABHFT ABHMW ABJNI ABLBI ABMVS ABOCM ABPPZ ABQRX ABUCX ABUFD ACBEA ACGFO ACGFS ACGOD ACIWK ACJ ACKOT ACNCT ACPRK ACS ADHLV AEESW AENEX AFEFF AFRAH AGXLV AHGAQ ALMA_UNASSIGNED_HOLDINGS AQSVZ BAANH BKOMP CS3 CUPRZ D0L EBS ED~ F5P GGK GNL IH9 IHE JG~ KZ1 LMP P2P PQQKQ ROL RXW TAE TN5 UHB UI2 UKR VF5 VG9 W1F WH7 X6Y XSW YZZ ZCA ~02 |
| ID | FETCH-LOGICAL-a386t-e2141808180b639dfc07155c3ce300d656cd289c02ef6fecb32c9bb3d7ef56242 |
| IEDL.DBID | 7X8 |
| ISICitedReferencesCount | 1 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=001082560500001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 1520-6882 |
| IngestDate | Sun Nov 09 12:51:22 EST 2025 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 41 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-a386t-e2141808180b639dfc07155c3ce300d656cd289c02ef6fecb32c9bb3d7ef56242 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PQID | 2874266394 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_2874266394 |
| PublicationCentury | 2000 |
| PublicationDate | 2023-10-17 |
| PublicationDateYYYYMMDD | 2023-10-17 |
| PublicationDate_xml | – month: 10 year: 2023 text: 2023-10-17 day: 17 |
| PublicationDecade | 2020 |
| PublicationTitle | Analytical chemistry (Washington) |
| PublicationYear | 2023 |
| SSID | ssj0011016 |
| Score | 2.441058 |
| Snippet | Dengue is a viral disease transmitted by Aedes aegypti mosquitoes. According to the World Health Organization, about half of the world's population is at risk... |
| SourceID | proquest |
| SourceType | Aggregation Database |
| StartPage | 15217 |
| Title | Antibody Profiling of Dengue Severities Using Flavivirus Protein Microarrays |
| URI | https://www.proquest.com/docview/2874266394 |
| Volume | 95 |
| WOSCitedRecordID | wos001082560500001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LSwMxEA5qBfXgoyq-ieB1a5N0N7snKdXioZaCD3or2SQrC7qru22h_96ZuEsFL4LXIYdkkswjM_k-Qq4ig7Di3Hoy1JCgaAZ3Dvy0J0GqYss5c92ELwM5HIbjcTSqHtzKqq2ytonOUJtc4xv5NeKygzMRUefm49ND1iisrlYUGqukISCUwVMtx8sqAmamDi8VUyQIJeuvc5xdK122FGwVaOa9JTSIAv-XOXY-pr_z39ntku0quqTd7-OwR1Zs1iQbvZrUrUm2fuAP7pNBN5umcW4WdOSou0FI84Te2ux1ZumjhWPuAFepayyg_Tc1T-dpMStxPNJk0gfs51NFoRblAXnu3z317r2KXsFTIgymnuWsw5B4I2zHMHOTaAg3fF8LbUW7bSDQ0wbSMdCNTYLE6lhwHcWxMNImPn4rOSRrWZ7ZI0KFgDgpZJIZZSDf43GYBBq8I4KVCRnxY3JZq20CC8aahMpsPisnS8Wd_GHMKdlEunf0HUyekUYCV9Sek3U9n6ZlceF2_wttCbjd |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Antibody+Profiling+of+Dengue+Severities+Using+Flavivirus+Protein+Microarrays&rft.jtitle=Analytical+chemistry+%28Washington%29&rft.au=Tsai%2C+Pei-Shan&rft.au=Du%2C+Pin-Xian&rft.au=Keskin%2C+Batuhan+Birol&rft.au=Lee%2C+Nan-Yao&rft.date=2023-10-17&rft.issn=1520-6882&rft.eissn=1520-6882&rft.volume=95&rft.issue=41&rft.spage=15217&rft_id=info:doi/10.1021%2Facs.analchem.3c02165&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1520-6882&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1520-6882&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1520-6882&client=summon |