Effect of Poly(γ-glutamic acid) on the Physiological Responses and Calcium Signaling of Rape Seedlings (Brassica napus L.) under Cold Stress
Cold stress adversely affects plant growth and development. Poly(γ-glutamic acid) (γ-PGA) is a potential plant growth regulator that may be an effective cryoprotectant that prevents crops from damage during cold weather. In this study, the effects of γ-PGA on the physiological responses of rape seed...
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| Vydané v: | Journal of agricultural and food chemistry Ročník 63; číslo 48; s. 10399 |
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| Hlavní autori: | , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | English |
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United States
09.12.2015
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| ISSN: | 1520-5118, 1520-5118 |
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| Abstract | Cold stress adversely affects plant growth and development. Poly(γ-glutamic acid) (γ-PGA) is a potential plant growth regulator that may be an effective cryoprotectant that prevents crops from damage during cold weather. In this study, the effects of γ-PGA on the physiological responses of rape seedlings subject to cold stress were investigated using hydroponic experiments. We determined that the malondialdehyde content was decreased by 33.4% and the proline content was increased by 62.5% by γ-PGA after 144 h under cold stress. Antioxidant enzymes activities were also evidently enhanced after treatment with γ-PGA. These responses counteracted increases in the fresh weight and chlorophyll content of rape seedlings, which increased by 24.5 and 50.9%, respectively, after 144 h, which meant that growth inhibition caused by cold was mitigated by γ-PGA. Our results also showed that γ-PGA also regulated Ca(2+) concentrations in the cytoplasm and calcium-dependent protein kinases, which are associated with cold resistance. In conclusion, we suggest that the Ca(2+)/CPKs signal pathway is involved in the γ-PGA-mediated enhancement of cold resistance in rape seedlings. |
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| AbstractList | Cold stress adversely affects plant growth and development. Poly(γ-glutamic acid) (γ-PGA) is a potential plant growth regulator that may be an effective cryoprotectant that prevents crops from damage during cold weather. In this study, the effects of γ-PGA on the physiological responses of rape seedlings subject to cold stress were investigated using hydroponic experiments. We determined that the malondialdehyde content was decreased by 33.4% and the proline content was increased by 62.5% by γ-PGA after 144 h under cold stress. Antioxidant enzymes activities were also evidently enhanced after treatment with γ-PGA. These responses counteracted increases in the fresh weight and chlorophyll content of rape seedlings, which increased by 24.5 and 50.9%, respectively, after 144 h, which meant that growth inhibition caused by cold was mitigated by γ-PGA. Our results also showed that γ-PGA also regulated Ca(2+) concentrations in the cytoplasm and calcium-dependent protein kinases, which are associated with cold resistance. In conclusion, we suggest that the Ca(2+)/CPKs signal pathway is involved in the γ-PGA-mediated enhancement of cold resistance in rape seedlings.Cold stress adversely affects plant growth and development. Poly(γ-glutamic acid) (γ-PGA) is a potential plant growth regulator that may be an effective cryoprotectant that prevents crops from damage during cold weather. In this study, the effects of γ-PGA on the physiological responses of rape seedlings subject to cold stress were investigated using hydroponic experiments. We determined that the malondialdehyde content was decreased by 33.4% and the proline content was increased by 62.5% by γ-PGA after 144 h under cold stress. Antioxidant enzymes activities were also evidently enhanced after treatment with γ-PGA. These responses counteracted increases in the fresh weight and chlorophyll content of rape seedlings, which increased by 24.5 and 50.9%, respectively, after 144 h, which meant that growth inhibition caused by cold was mitigated by γ-PGA. Our results also showed that γ-PGA also regulated Ca(2+) concentrations in the cytoplasm and calcium-dependent protein kinases, which are associated with cold resistance. In conclusion, we suggest that the Ca(2+)/CPKs signal pathway is involved in the γ-PGA-mediated enhancement of cold resistance in rape seedlings. Cold stress adversely affects plant growth and development. Poly(γ-glutamic acid) (γ-PGA) is a potential plant growth regulator that may be an effective cryoprotectant that prevents crops from damage during cold weather. In this study, the effects of γ-PGA on the physiological responses of rape seedlings subject to cold stress were investigated using hydroponic experiments. We determined that the malondialdehyde content was decreased by 33.4% and the proline content was increased by 62.5% by γ-PGA after 144 h under cold stress. Antioxidant enzymes activities were also evidently enhanced after treatment with γ-PGA. These responses counteracted increases in the fresh weight and chlorophyll content of rape seedlings, which increased by 24.5 and 50.9%, respectively, after 144 h, which meant that growth inhibition caused by cold was mitigated by γ-PGA. Our results also showed that γ-PGA also regulated Ca(2+) concentrations in the cytoplasm and calcium-dependent protein kinases, which are associated with cold resistance. In conclusion, we suggest that the Ca(2+)/CPKs signal pathway is involved in the γ-PGA-mediated enhancement of cold resistance in rape seedlings. |
| Author | Ding, Yan Xu, Zongqi Tang, Bao Li, Huashan Feng, Xiaohai Lei, Peng Zhang, Yunxia Xu, Hong |
| Author_xml | – sequence: 1 givenname: Peng surname: Lei fullname: Lei, Peng organization: College of Food Science and Light Industry, Nanjing Tech University , Nanjing 211816, China – sequence: 2 givenname: Zongqi surname: Xu fullname: Xu, Zongqi organization: College of Food Science and Light Industry, Nanjing Tech University , Nanjing 211816, China – sequence: 3 givenname: Yan surname: Ding fullname: Ding, Yan organization: Nanjing Shineking Biological Technology Company, Ltd., Nanjing 210000, China – sequence: 4 givenname: Bao surname: Tang fullname: Tang, Bao organization: College of Food Science and Light Industry, Nanjing Tech University , Nanjing 211816, China – sequence: 5 givenname: Yunxia surname: Zhang fullname: Zhang, Yunxia organization: College of Food Science and Light Industry, Nanjing Tech University , Nanjing 211816, China – sequence: 6 givenname: Huashan surname: Li fullname: Li, Huashan organization: College of Food Science and Light Industry, Nanjing Tech University , Nanjing 211816, China – sequence: 7 givenname: Xiaohai surname: Feng fullname: Feng, Xiaohai organization: College of Food Science and Light Industry, Nanjing Tech University , Nanjing 211816, China – sequence: 8 givenname: Hong surname: Xu fullname: Xu, Hong organization: College of Food Science and Light Industry, Nanjing Tech University , Nanjing 211816, China |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/26585291$$D View this record in MEDLINE/PubMed |
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| Keywords | proline Ca2+ fluctuation cold stress antioxidant enzymes Ca2+/CPKs signal pathway poly(γ-glutamic acid) |
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| SubjectTerms | Brassica napus - genetics Brassica napus - metabolism Calcium - metabolism Catalase - metabolism Chlorophyll - metabolism Cold Temperature Malondialdehyde - metabolism Plant Leaves - metabolism Plant Proteins - metabolism Polyglutamic Acid - analogs & derivatives Seedlings - genetics Seedlings - growth & development Seedlings - metabolism Signal Transduction Superoxide Dismutase - metabolism |
| Title | Effect of Poly(γ-glutamic acid) on the Physiological Responses and Calcium Signaling of Rape Seedlings (Brassica napus L.) under Cold Stress |
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