Search Results - Protein Array Analysis/methods*

Anticitrullinated protein/peptide antibody multiplexing defines an extended group of ACPA-positive rheumatoid arthritis patients with distinct genetic and environmental determinants by Rönnelid, Johan, Hansson, Monika, Mathsson-Alm, Linda, Cornillet, Martin, Reed, Evan, Jakobsson, Per-Johan, Alfredsson, Lars, Holmdahl, Rikard, Skriner, Karl, Serre, Guy, Lundberg, Karin, Klareskog, Lars

“…IntroductionThe second generation anticycliccitrullinated peptide (anti-CCP2) assay detects the majority but not all anticitrullinated protein/peptide antibodies (ACPA…”
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Statistical Methods for Analysis of Protein Microarray Data Using R by Chung, Yunro

“…, visualization, and power analysis. The R code used to perform the statistical analyses will be provided.This chapter aims to provide statistical methods for analyzing protein microarray data…”
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Realizing the promise of reverse phase protein arrays for clinical, translational, and basic research: a workshop report: the RPPA (Reverse Phase Protein Array) society by Akbani, Rehan, Becker, Karl-Friedrich, Carragher, Neil, Goldstein, Ted, de Koning, Leanne, Korf, Ulrike, Liotta, Lance, Mills, Gordon B, Nishizuka, Satoshi S, Pawlak, Michael, Petricoin, 3rd, Emanuel F, Pollard, Harvey B, Serrels, Bryan, Zhu, Jingchun

“…Reverse phase protein array (RPPA) technology introduced a miniaturized "antigen-down" or "dot-blot" immunoassay suitable for quantifying the relative, semi-quantitative or quantitative…”
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Characterization of Human Cancer Cell Lines by Reverse-phase Protein Arrays by Li, Jun, Zhao, Wei, Akbani, Rehan, Liu, Wenbin, Ju, Zhenlin, Ling, Shiyun, Vellano, Christopher P, Roebuck, Paul, Yu, Qinghua, Eterovic, A Karina, Byers, Lauren A, Davies, Michael A, Deng, Wanleng, Gopal, Y N Vashisht, Chen, Guo, von Euw, Erika M, Slamon, Dennis, Conklin, Dylan, Heymach, John V, Gazdar, Adi F, Minna, John D, Myers, Jeffrey N, Lu, Yiling, Mills, Gordon B, Liang, Han

“… Using reverse-phase protein arrays, we measured expression levels of ∼230 key cancer-related proteins…”
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SARS-CoV-2 proteome microarray for global profiling of COVID-19 specific IgG and IgM responses by Jiang, He-wei, Li, Yang, Zhang, Hai-nan, Wang, Wei, Yang, Xiao, Qi, Huan, Li, Hua, Men, Dong, Zhou, Jie, Tao, Sheng-ce

“… Here we construct a SARS-CoV-2 proteome microarray containing 18 out of the 28 predicted proteins and apply it to the characterization of the IgG and IgM antibodies responses in the sera from 29 convalescent patients…”
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In-situ ultra-sensitive infrared absorption spectroscopy of biomolecule interactions in real time with plasmonic nanoantennas by Adato, Ronen, Altug, Hatice

“… Its molecular specificity renders the technique sensitive to the subtle conformational changes exhibited by proteins in response to a variety of stimuli…”
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Serial dilution curve: a new method for analysis of reverse phase protein array data by Zhang, Li, Wei, Qingyi, Mao, Li, Liu, Wenbin, Mills, Gordon B., Coombes, Kevin

“…Reverse phase protein arrays (RPPAs) are a powerful high-throughput tool for measuring protein concentrations in a large number of samples…”
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Highly multiplexed simultaneous detection of RNAs and proteins in single cells by Frei, Andreas P, Bava, Felice-Alessio, Zunder, Eli R, Hsieh, Elena W Y, Chen, Shih-Yu, Nolan, Garry P, Gherardini, Pier Federico

“…PLAYR (proximity ligation assay for RNA) enables highly multiplexed transcript quantification in combination with protein marker detection in single cells using flow or mass cytometry…”
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Developments and Applications of Functional Protein Microarrays by Syu, Guan-Da, Dunn, Jessica, Zhu, Heng

“…Protein microarrays are crucial tools in the study of proteins in an unbiased, high-throughput manner, as they allow for characterization of up to thousands of individually purified proteins in parallel…”
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Nanobodies and recombinant binders in cell biology by Helma, Jonas, Cardoso, M Cristina, Muyldermans, Serge, Leonhardt, Heinrich

“…Antibodies are key reagents to investigate cellular processes. The development of recombinant antibodies and binders derived from natural protein scaffolds…”
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A human lectin array for characterizing host-pathogen interactions by Benjamin, Stefi V., Jégouzo, Sabine A.F., Lieng, Chloe, Daniels, Connor, Coispeau, Marine, Lau, Rikin J., Kim, Suyeon, Metaxa, Yasmine, Philpott, James, Li, Tiannuo, Dai, Chao, Wang, Xin, Newby, Maddy L., Pier, Gerald B., Crispin, Max, Clements, Abigail, Taylor, Maureen E., Drickamer, Kurt

“…A human lectin array has been developed to probe the interactions of innate immune receptors with pathogenic and commensal microorganism…”
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Proteome-wide microarray-based screening of PAR-binding proteins by Kang, Bong Gu, Kang, Sung-Ung, Jin Kim, Jae, Kwon, Ji-Sun, Gagné, Jean-Philippe, Yun Lee, Seo, Kim, Soyeon, Lee, Karl S, Ha, Shinwon, Seop Jeong, Jun, Lee, Yun-Il, Zhu, Heng, Kim, Dongsan, Poirier, Guy G, Chul Kang, Ho, Dawson, Valina L, Dawson, Ted M

“… Here we performed a PAR-binding screen using a human protein microarray that covers most of the human proteome to characterize the non-covalent binding PARylome…”
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An Interactive Database for the Assessment of Histone Antibody Specificity by Rothbart, Scott B, Dickson, Bradley M, Raab, Jesse R, Grzybowski, Adrian T, Krajewski, Krzysztof, Guo, Angela H, Shanle, Erin K, Josefowicz, Steven Z, Fuchs, Stephen M, Allis, C David, Magnuson, Terry R, Ruthenburg, Alexander J, Strahl, Brian D

“…). Here we debut the Histone Antibody Specificity Database (http://www.histoneantibodies.com), an online and expanding resource cataloging the behavior of widely used, commercially available histone antibodies by peptide microarray…”
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Direct Profiling of Extracellular Vesicle Surface Markers in Plasma: A Proof-of-Concept Study by Clegg, Lee-Ann, Jensen, Rebecca Best, Bæk, Rikke, Mumm, Birgitte Hviid, Sørensen, Maria Kjølhede, Blauenfeldt, Rolf Ankerlund, Drasbek, Kim Ryun, Jørgensen, Malene Møller

“…During the last decade, non-invasive methods such as liquid biopsy have increasingly replaced invasive techniques for diagnosing and monitoring diseases…”
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Disease-specific molecular events in cortical multiple sclerosis lesions by Fischer, Marie Therese, Wimmer, Isabella, Höftberger, Romana, Gerlach, Susanna, Haider, Lukas, Zrzavy, Tobias, Hametner, Simon, Mahad, Don, Binder, Christoph J, Krumbholz, Markus, Bauer, Jan, Bradl, Monika, Lassmann, Hans

“…Cortical lesions constitute an important part of multiple sclerosis pathology. Although inflammation appears to play a role in their formation, the mechanisms…”
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Multiplexed sandwich immunoassays using electrochemiluminescence imaging resolved at the single bead level by Deiss, Frédérique, LaFratta, Christopher N, Symer, Matthew, Blicharz, Timothy M, Sojic, Neso, Walt, David R

“…A new class of bead-based microarray that uses electrogenerated chemiluminescence (ECL…”
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Plasmodium falciparum serology: A comparison of two protein production methods for analysis of antibody responses by protein microarray by Oulton, Tate, Obiero, Joshua, Rodriguez, Isabel, Ssewanyana, Isaac, Dabbs, Rebecca A., Bachman, Christine M., Greenhouse, Bryan, Drakeley, Chris, Felgner, Phil L., Stone, Will, Tetteh, Kevin K. A.

“…The evaluation of protein antigens as putative serologic biomarkers of infection has increasingly shifted to high-throughput, multiplex approaches such as the protein microarray…”
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Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging by Zhu, Ling, Wang, Kun, Cui, Jian, Liu, Huan, Bu, Xiangli, Ma, Huailei, Wang, Weizhi, Gong, He, Lausted, Christopher, Hood, Leroy, Yang, Guang, Hu, Zhiyuan

“… Utilizing surface plasmon resonance imaging (SPRi) in combination with antibody microarrays specific to the extracellular domains of exosome membrane proteins, exosomes in tumor cell culture medium can be quantitatively…”
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Immobilization of Protein Macromolecules in Biochip Cells Made of Various Polymers by Shtylev, G F, Shishkin, I Yu, Vasiliskov, V A, Barsky, V E, Kuznetsova, V E, Shershov, V E, Polyakov, S A, Miftakhov, R A, Butvilovskaya, V I, Zasedateleva, O A, Chudinov, A V

“…Microarrays with immobilized protein probes are used to analyze protein samples. Selection of materials for biochip fabrication, functionalization…”
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Activity-based protein profiling in bacteria: Applications for identification of therapeutic targets and characterization of microbial communities by Keller, Laura J., Babin, Brett M., Lakemeyer, Markus, Bogyo, Matthew

“…Activity-based protein profiling (ABPP) is a robust chemoproteomic technique that uses activity-based probes to globally measure endogenous enzymatic activity in complex proteomes…”
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