Search Results - "protein quantification"

Abstract 2475 Exploring Alternative Dyes for Enhanced Sensitivity and Specificity in the Bradford Protein Assay by Mckenzie, Brooklynn, Boles, Jeffrey

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Proteomics Standards Initiative: Fifteen Years of Progress and Future Work by Deutsch, Eric W, Orchard, Sandra, Binz, Pierre-Alain, Bittremieux, Wout, Eisenacher, Martin, Hermjakob, Henning, Kawano, Shin, Lam, Henry, Mayer, Gerhard, Menschaert, Gerben, Perez-Riverol, Yasset, Salek, Reza M, Tabb, David L, Tenzer, Stefan, Vizcaíno, Juan Antonio, Walzer, Mathias, Jones, Andrew R

“…The Proteomics Standards Initiative (PSI) of the Human Proteome Organization (HUPO) has now been developing and promoting open community standards and software…”
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Spectral counting label-free proteomics by Arike, Liisa, Peil, Lauri

“…Label-free proteome quantification methods used in bottom-up mass-spectrometry based proteomics are gaining more popularity as they are easy to apply and can…”
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Gene‐specific correlation of RNA and protein levels in human cells and tissues by Edfors, Fredrik, Danielsson, Frida, Hallström, Björn M, Käll, Lukas, Lundberg, Emma, Pontén, Fredrik, Forsström, Björn, Uhlén, Mathias

“…An important issue for molecular biology is to establish whether transcript levels of a given gene can be used as proxies for the corresponding protein levels…”
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MSstatsTMT: Statistical Detection of Differentially Abundant Proteins in Experiments with Isobaric Labeling and Multiple Mixtures by Huang, Ting, Choi, Meena, Tzouros, Manuel, Golling, Sabrina, Pandya, Nikhil Janak, Banfai, Balazs, Dunkley, Tom, Vitek, Olga

“…Tandem mass tag (TMT) is a multiplexing technology widely-used in proteomic research. It enables relative quantification of proteins from multiple biological…”
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Efficient quantification of emicizumab by LC-MS/MS using protein G bead enrichment by Ma, Xiaoli, He, Xiaoshuang, Liu, Yu, Zhao, Juntao, Chen, Wenkai, Hu, Fengmei, Lu, Qiuya, Dai, Jing

“…Emicizumab, a bispecific antibody mimicking factor VIII(FVIII), has emerged as a cornerstone therapy for patients with hemophilia A, including those both with…”
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A direct high-throughput protein quantification strategy facilitates discovery and characterization of a celastrol-derived BRD4 degrader by Payne, N Connor, Maksoud, Semer, Tannous, Bakhos A, Mazitschek, Ralph

“…We describe a generalizable time-resolved Förster resonance energy transfer (TR-FRET)-based platform to profile the cellular action of heterobifunctional…”
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Tracking and Measuring Local Protein Synthesis In Vivo by Kays, Ibrahim, Chen, Brian E

“…Detecting when and how much a protein molecule is synthesized is important for understanding cell function, but current methods either cannot be performed in…”
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IQuant: An automated pipeline for quantitative proteomics based upon isobaric tags by Wen, Bo, Zhou, Ruo, Feng, Qiang, Wang, Quanhui, Wang, Jun, Liu, Siqi

“…Quantitative proteomics technology based on isobaric tags is playing an important role in proteomic investigation. In this paper, we present an automated…”
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An accurate and sensitive Coomassie Brilliant Blue G-250-based assay for protein determination by Grintzalis, Konstantinos, Georgiou, Christos D., Schneider, Yves-Jacques

“…In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous…”
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Protein quantification by bicinchoninic acid (BCA) assay follows complex kinetics and can be performed at short incubation times by Cortés-Ríos, Javiera, Zárate, Ana María, Figueroa, Juan David, Medina, Joaquín, Fuentes-Lemus, Eduardo, Rodríguez-Fernández, María, Aliaga, Margarita, López-Alarcón, Camilo

“…Amongst the available methodologies for protein determination, the bicinchoninic acid (BCA) assay highlights for its simplicity, sensitivity, repeatability and…”
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Wash-free chemiluminescence imaging sensing system for portable protein assay and high-throughput screening of PCSK9 inhibitors by Yang, Ruyu, Chen, Enping, Li, Li, Wu, Liuying, Li, Huijun, Li, Ping, Zong, Chen

“…The inhibition of the proprotein convertase subtilisin/kexin type 9 (PCSK9) expression can reduce the circulating low-density lipoprotein cholesterol levels…”
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µPAD-based colorimetric nanogold aptasensor for CRP and IL-6 detection as sepsis biomarkers by Malekmohamadi, Marjan, Mirzaei, Samira, Rezayan, Ali Hossein, Abbasi, Vahid, Abouei Mehrizi, Ali

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A nanozyme-linked immunosorbent assay for dual-modal colorimetric and ratiometric fluorescent detection of cardiac troponin I by Miao, Luyang, Jiao, Lei, Tang, Qiaorong, Li, He, Zhang, Lianhua, Wei, Qin

“…•A new kind of robust nanozyme-linked immunosorbent assay has been developed.•The proposed nanozyme-linked immunosorbent assay can achieve ratiometric…”
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Cytotoxicity and toxicoproteomics analysis of thiazolidinedione exposure in human‐derived cardiomyocytes by Al Sultan, Abdullah, Rattray, Zahra, Rattray, Nicholas J. W.

“…Thiazolidinediones (TZDs) (e.g. pioglitazone and rosiglitazone), known insulin sensitiser agents for type II diabetes mellitus, exhibit controversial effects…”
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Quantitative Targeted Absolute Proteomics for Better Characterization of an In Vitro Human Blood–Brain Barrier Model Derived from Hematopoietic Stem Cells by Dehouck, Marie-Pierre, Tachikawa, Masanori, Hoshi, Yutaro, Omori, Kotaro, Maurage, Claude-Alain, Strecker, Guillaume, Dehouck, Lucie, Boucau, Marie-Christine, Uchida, Yasuo, Gosselet, Fabien, Terasaki, Tetsuya, Karamanos, Yannis

“…We previously developed an in vitro model of the human blood–brain barrier (BBB) based on the use of endothelial cells derived from CD34+-hematopoietic stem…”
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Identification and Quantification of Major Faba Bean Seed Proteins by Warsame, Ahmed O, Michael, Nicholas, O'Sullivan, Donal M, Tosi, Paola

“…Faba bean ( ) holds great importance for human and animal nutrition for its high protein content. However, better understanding of its seed protein composition…”
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Seaweed as an alternative protein source: Prospective protein extraction technologies by de Souza Celente, Gleison, Sui, Yixing, Acharya, Parag

“…Demand for food production has increased due to population growth. The negative environmental impact of animal agriculture necessitates the search for…”
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Quantitative, high-resolution proteomics for data-driven systems biology by Cox, Jürgen, Mann, Matthias

“…Systems biology requires comprehensive data at all molecular levels. Mass spectrometry (MS)-based proteomics has emerged as a powerful and universal method for…”
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Development and validation of signature peptide-based isotope dilution liquid chromatography-mass spectrometry for bovine lactoferrin purity assessment by Yan, Jingjing, Yang, Mengrui, Wang, Min, Han, Yiyi, Zhou, Jian, Ma, Yingqing, Wang, Tongtong, Li, Liang

“…[Display omitted] •Signature peptide-based ID-LC-MS/MS for quantification of bovine lactoferrin was reported.•The two-stage IDMS that included the…”
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