Výsledky vyhľadávania - "Reverse Transcriptase Polymerase Chain Reaction/methods"

Saliva as a Noninvasive Specimen for Detection of SARS-CoV-2 Autor Williams, Eloise, Bond, Katherine, Zhang, Bowen, Putland, Mark, Williamson, Deborah A

Zistit podrobnosti o prístupe

Sensitivity assessment of droplet digital PCR for SARS-CoV-2 detection Autor Falzone, Luca, Musso, Nicolò, Gattuso, Giuseppe, Bongiorno, Dafne, Palermo, Concetta Ilenia, Scalia, Guido, Libra, Massimo, Stefani, Stefania

“…Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the gold standard method for the diagnosis of COVID-19 infection. Due to…”
Získať plný text

One-step real-time RT-PCR assays for serotyping dengue virus in clinical samples Autor Alm, Erik, Lindegren, Gunnel, Falk, Kerstin Ingrid, Lagerqvist, Nina

“…Background Dengue is one of the leading causes of morbidity in tropical and subtropical regions and infection with any of the four dengue virus serotypes…”
Získať plný text

Endogenous RNase inhibitor contributes to stability of RNA in crude cell lysates: Applicability to RT-qPCR Autor Wang, Xiao, Teferedegne, Belete, Shatzkes, Kenneth, Tu, Wei, Murata, Haruhiko

“…Crude cell lysates are increasingly used as input for direct analysis by reverse transcription quantitative PCR (RT-qPCR), particularly for high-throughput…”
Získať plný text

Proenkephalin expression and enkephalin release are widely observed in non-neuronal tissues Autor Denning, Gerene M., Ackermann, Laynez W., Barna, Thomas J., Armstrong, John G., Stoll, Lynn L., Weintraub, Neal L., Dickson, Eric W.

“…Enkephalins are opioid peptides that are found at high levels in the brain and endocrine tissues. Studies have shown that enkephalins play an important role in…”
Získať plný text

Intraflagellar transport genes are essential for differentiation and survival of vertebrate sensory neurons Autor Tsujikawa, Motokazu, Malicki, Jarema

“…Cilia play diverse roles in vertebrate and invertebrate sensory neurons. We show that a mutation of the zebrafish oval (ovl) locus affects a component of the…”
Zistit podrobnosti o prístupe

Acid-sensing ion channel 3 expressed in type B synoviocytes and chondrocytes modulates hyaluronan expression and release Autor Kolker, S J, Walder, R Y, Usachev, Y, Hillman, J, Boyle, D L, Firestein, G S, Sluka, K A

“…Background Rheumatoid arthritis is an inflammatory disease marked by intra-articular decreases in pH, aberrant hyaluronan regulation and destruction of bone…”
Získať plný text

The potassium channels Kv1.5 and Kv1.3 modulate distinct functions of microglia Autor Pannasch, Ulrike, Färber, Katrin, Nolte, Christiane, Blonski, Mary, Yan Chiu, Shing, Messing, Albee, Kettenmann, Helmut

“…Activation of microglia by LPS leads to an induction of cytokine and NO release, reduced proliferation and increased outward K + conductance, the latter…”
Získať plný text

Bag1 is essential for differentiation and survival of hematopoietic and neuronal cells Autor Götz, Rudolf, Wiese, Stefan, Takayama, Shinichi, Camarero, Guadalupe C, Rossoll, Wilfried, Schweizer, Ulrich, Troppmair, Jakob, Jablonka, Sibylle, Holtmann, Bettina, Reed, John C, Rapp, Ulf R, Sendtner, Michael

“…Bag1 is a cochaperone for the heat-shock protein Hsp70 that interacts with C-Raf, B-Raf, Akt, Bcl-2, steroid hormone receptors and other proteins. Here we use…”
Získať plný text

Gene transfer of soluble interleukin-17 receptor prolongs cardiac allograft survival in a rat model Autor Li, Jianping, Simeoni, Eleonora, Fleury, Sylvain, Dudler, Jean, Fiorini, Emma, Kappenberger, Lukas, von Segesser, Ludwig K, Vassalli, Giuseppe

“…Interleukin-17 (IL-17), a potent proinflammatory cytokine, has been implicated in allograft rejection. We analyzed the efficacy of an adenoviral vector…”
Zistit podrobnosti o prístupe

From gene expression to serum proteins: biomarker discovery in ankylosing spondylitis Autor Haroon, N, Tsui, F W L, O’Shea, F D, Chiu, B, Tsui, H W, Zhang, H, Marshall, K W, Inman, R D

“…Objectives:Studying post-infliximab gene expression changes could provide insights into the pathogenesis of ankylosing spondylitis (AS).Methods:Gene expression…”
Získať plný text

Postsynaptic mechanisms underlying responsiveness of amygdaloid neurons to cholecystokinin are mediated by a transient receptor potential-like current Autor Meis, Susanne, Munsch, Thomas, Sosulina, Ludmila, Pape, Hans-Christian

“…Projection neurons of mouse basolateral amygdala responded to CCK with an inward current at a holding potential of − 70 mV. This response was mediated by CCK 2…”
Získať plný text

VEGF and odontoblast-like cells: Stimulation by low frequency ultrasound Autor Scheven, B.A., Man, J., Millard, J.L., Cooper, P.R., Lea, S.C., Walmsley, A.D., Smith, A.J.

“…Vascular endothelial growth factor (VEGF) has been implicated in the regulation of dental pulp and dentine repair. Therapeutic ultrasound was shown to be…”
Získať plný text

Reference genes for canine skin when using quantitative real-time PCR Autor Wood, Shona H., Clements, Dylan N., McEwan, Neil A., Nuttall, Tim, Carter, Stuart D.

“…Quantitative real-time PCR (qPCR) facilitates the quantification of mRNA expression. Accurate qPCR analysis of gene expression requires the normalisation of…”
Získať plný text

Rating of putative housekeeping genes for quantitative gene expression analysis in cyclic and early pregnant equine endometrium Autor Kayis, Seyit A, Atli, Mehmet O, Kurar, Ercan, Bozkaya, Faruk, Semacan, Ahmet, Aslan, Selim, Guzeloglu, Aydin

“…The aim was an evaluation of a set of housekeeping genes (HKGs) to be used in the normalization of gene expression in the equine endometrium…”
Získať plný text

Expression of cysteinyl leukotriene receptor 1 in human traumatic brain injury and brain tumors Autor Zhang, Wei-Ping, Hu, Hua, Zhang, Lei, Ding, Wei, Yao, Hong-Tian, Chen, Ke-Da, Sheng, Wen-Wen, Chen, Zhong, Wei, Er-Qing

“…Cysteinyl leukotrienes (CysLTs) are potent proinflammatory mediators. CysLT receptor 1 (CysLT 1) is one of the two CysLT receptors that has been cloned…”
Získať plný text

Defining Suitable Reference Genes for RT-qPCR Analysis on Intestinal Epithelial Cells Autor Sirakov, Maria, Borra, Marco, Cambuli, Francesca Maria, Plateroti, Michelina

“…The study of the mammalian intestinal epithelium concerns several aspects of cellular and molecular biology. In fact, most of these studies aim to define…”
Získať plný text

Reliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin subtypes Autor Li, Olive T.W., Barr, Ian, Leung, Connie Y.H., Chen, Honglin, Guan, Yi, Peiris, J.S. Malik, Poon, Leo L.M.

“…Realizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based…”
Získať plný text

Development of SYBR green I based one-step real-time RT-PCR assay for the detection and differentiation of very virulent and classical strains of infectious bursal disease virus Autor Kong, Lih Ling, Omar, Abdul Rahman, Hair Bejo, Mohd, Ideris, Aini, Tan, Sheau Wei

“…A SYBR Green I based one-step real-time reverse transcriptase polymerase chain reaction was developed for the detection and differentiation of very virulent…”
Získať plný text

Development and Assessment of a Quantitative Reverse Transcription-PCR Assay for Simultaneous Measurement of Four Amplicons Autor Grace, Marcy B, McLeland, Christopher B, Gagliardi, Steven J, Smith, Jeffrey M, Jackson, William E., III, Blakely, William F

“…Background: High-throughput and forward-deployable biological dosimetry capabilities are required for tactical and medical decisions after radiologic events…”
Získať plný text