Suchergebnisse - "Reverse Transcriptase Polymerase Chain Reaction"

Comparison of Different Reverse Transcriptase–Polymerase Chain Reaction–Based Methods for Wastewater Surveillance of SARS-CoV-2: Exploratory Study von Länsivaara, Annika, Lehto, Kirsi-Maarit, Hyder, Rafiqul, Janhonen, Erja Sinikka, Lipponen, Anssi, Heikinheimo, Annamari, Pitkänen, Tarja, Oikarinen, Sami

“… The most used methods for detecting SARS-CoV-2 in wastewater are quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR …”
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Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR von Smyrlaki, Ioanna, Ekman, Martin, Lentini, Antonio, Rufino de Sousa, Nuno, Papanicolaou, Natali, Vondracek, Martin, Aarum, Johan, Safari, Hamzah, Muradrasoli, Shaman, Rothfuchs, Antonio Gigliotti, Albert, Jan, Högberg, Björn, Reinius, Björn

“… Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed by reverse transcription …”
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Viral Detection by Reverse Transcriptase Polymerase Chain Reaction in Upper Respiratory Tract and Metagenomic RNA Sequencing in Lower Respiratory Tract in Critically Ill Children With Suspected Lower Respiratory Tract Infection von Osborne, Christina M, Langelier, Charles, Kamm, Jack, Williamson, Kayla, Ambroggio, Lilliam, Reeder, Ron W, Locandro, Christopher, Kirk Harris, J, Wagner, Brandie D, Maddux, Aline B, Caldera, Saharai, Lyden, Amy, Soesanto, Victoria, Simões, Eric A F, Leroue, Matthew K, Carpenter, Todd C, Hall, Mark W, Zuppa, Athena F, Carcillo, Joseph A, Meert, Kathleen L, Pollack, Murray M, McQuillen, Patrick S, Notterman, Daniel A, DeRisi, Joseph, Mourani, Peter M

“… Diagnosis is typically confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) of nasopharyngeal specimens in hospitalized patients …”
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Reliable gene expression analysis by reverse transcription-quantitative PCR: reporting and minimizing the uncertainty in data accuracy von Remans, Tony, Keunen, Els, Bex, Geert Jan, Smeets, Karen, Vangronsveld, Jaco, Cuypers, Ann

“… Additionally, even experiments that use appropriate procedures remain subject to individual variation that statistical methods cannot correct …”
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Efficacy of the Examination of Saliva Sample by Reverse Transcriptase-Polymerase Chain Reaction in Detection of SARS-CoV-2 in Al-Fallujah City, Iraq von Al-Ani, Hind Q. Jameel, Al-Hayani, Noor N., Al-Ani, Raid M.

“… Saliva samples could be used as a non-invasive method to diagnose COVID-19. We aimed to assess the results of the reverse transcriptase-polymerase chain reaction (RT-PCR …”
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BD Veritor System Respiratory Syncytial Virus Rapid Antigen Detection Test: Point-of-Care Results in Primary Care Pediatric Offices Compared With Reverse Transcriptase Polymerase Chain Reaction and Viral Culture Methods von Schwartz, Richard H, Selvarangan, Rangaraj, Zissman, Edward N

“… During respiratory syncytial virus season, many children present to pediatric offices and urgent care medical facilities with cough, tachypnea, intercostal …”
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Identification of Prevalent Dengue Serotypes by Reverse Transcriptase Polymerase Chain Reaction and Correlation with Severity of Dengue as per the Recent World Health Organization Classification (2009) von Mehta, Tanmay K., Shah, Parul D.

“… Subjects and Methods: We tested 242 dengue NS-1 antigen ELISA-positive cases for serotyping by dengue reverse transcriptase-polymerase chain reaction (RT-PCR …”
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Diagnostic performances and thresholds: The key to harmonization in serological SARS-CoV-2 assays? von Plebani, Mario, Padoan, Andrea, Negrini, Davide, Carpinteri, Benedetta, Sciacovelli, Laura

“… (ELISA) assays. Sera from a total of 271 subjects, including 64 reverse transcription-polymerase chain reaction (RT-PCR …”
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Occurrence and transmission potential of asymptomatic and presymptomatic SARS-CoV-2 infections: A living systematic review and meta-analysis von Buitrago-Garcia, Diana, Egli-Gany, Dianne, Counotte, Michel J., Hossmann, Stefanie, Imeri, Hira, Ipekci, Aziz Mert, Salanti, Georgia, Low, Nicola

“… There is disagreement about the level of asymptomatic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We conducted a living systematic …”
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Sensitive detection of occult breast cancer by the reverse-transcriptase polymerase chain reaction von Datta, Y H, Adams, P T, Drobyski, W R, Ethier, S P, Terry, V H, Roth, M S

“… To improve on existing methods of detection, we have developed a reverse-transcriptase polymerase chain reaction (RT-PCR …”
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Loss of Sense of Smell and Taste among Reverse Transcriptase Polymerase Chain Reaction Positive COVID-19 Patients of a Tertiary Care Hospital: A Descriptive Cross-sectional Study von Shah, Rajeev, Koirala, Bipin, Gyawali, Sangya

“… Nearly half of the subjects about 99 (51.6%) had dysfunction in both taste and smell. Conclusions: The present study shows the strong association between the olfactory and gustatory dysfunction with that of COVID infection …”
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Prevalence studies of GB Virus-C infection using reverse transcriptase-polymerase chain reaction von Dawson, George J., Schlauder, George G., Pilot-Matias, Tami J., Thiele, Dwain, Leary, Thomas P., Murphy, Paul, Rosenblatt, Jon E., Simons, John N., Martinson, Francis E. A., Gutierrez, Robin A., Lentino, Joseph R., Pachucki, Constance, Muerhoff, A. Scott, Widell, Anders, Tegtmeier, Gary, Desai, Suresh, Mushahwar, Isa K.

“… Among the three recently described GB viruses (GBV‐A, GBV‐B, and GBV‐C), only GBV‐C has been linked to cryptogenic hepatitis in man. Because of the limited …”
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Comparison of Reverse Transcriptase Loop-Mediated Isothermal Amplification and Reverse Transcriptase Polymerase Chain Reaction for Detection of Prostate Specific Antigen von Almasi, Mohammad Amin, Esmaili, Marya

“… A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was developed and compared to reverse transcriptase polymerase chain reaction (RT-PCR …”
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Variables influencing the efficiency and interpretation of reverse transcription quantitative PCR (RT-qPCR): An empirical study using Bacteriophage MS2 von Miranda, Jaclyn A., Steward, Grieg F.

“… •Increasing total RNA and primer concentrations improved RT efficiency.•At high concentrations, random hexamers performed as well as gene-specific primers.•RNA …”
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Guidelines for Laboratory Diagnosis of Coronavirus Disease 2019 (COVID-19) in Korea von Hong, Ki Ho, Lee, Sang Won, Kim, Taek Soo, Huh, Hee Jae, Lee, Jaehyeon, Kim, So Yeon, Park, Jae Sun, Kim, Gab Jeong, Sung, Heungsup, Roh, Kyoung Ho, Kim, Jae Seok, Kim, Hyun Soo, Lee, Seung Tae, Seong, Moon Woo, Ryoo, Namhee, Lee, Hyukmin, Kwon, Kye Chul, Yoo, Cheon Kwon

“… These guidelines are based on other related domestic and international guidelines, as well as expert opinions and include the selection of test subjects, selection of specimens, diagnostic methods …”
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Differential roles of macrophages in diverse phases of skin repair von Lucas, Tina, Waisman, Ari, Ranjan, Rajeev, Roes, Jürgen, Krieg, Thomas, Müller, Werner, Roers, Axel, Eming, Sabine A

“… Influx of macrophages plays a crucial role in tissue repair. However, the precise function of macrophages during the healing response has remained a subject …”
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Children Have Similar Reverse Transcription Polymerase Chain Reaction Cycle Threshold for Severe Acute Respiratory Syndrome Coronavirus 2 in Comparison With Adults von Polese-Bonatto, Márcia, Sartor, Ivaine Tais Sauthier, Varela, Fernanda Hammes, Giannini, Gabriela Luchiari Tumioto, Azevedo, Thaís Raupp, Kern, Luciane Beatriz, Fernandes, Ingrid Rodrigues, Zavaglia, Gabriela Oliveira, de David, Caroline Nespolo, Santos, Amanda Paz, de Almeida, Walquiria Aparecida Ferreira, Porto, Victor Bertollo Gomes, Scotta, Marcelo Comerlato, Stein, Renato T

“… ) values among children with confirmed SARS-CoV-2 compared with that of adult subjects. Patients (from 2 months to ≤18 years of age and adults …”
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A Compositional Look at the Human Gastrointestinal Microbiome and Immune Activation Parameters in HIV Infected Subjects von Mutlu, Ece A., Keshavarzian, Ali, Losurdo, John, Swanson, Garth, Siewe, Basile, Forsyth, Christopher, French, Audrey, DeMarais, Patricia, Sun, Yan, Koenig, Lars, Cox, Stephen, Engen, Phillip, Chakradeo, Prachi, Abbasi, Rawan, Gorenz, Annika, Burns, Charles, Landay, Alan

“… 121 specimens were collected from 21 HIV positive and 22 control human subjects during colonoscopy …”
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Observation of circulating tumour cells in patients with non-small cell lung cancer by real-time fluorescent quantitative reverse transcriptase-polymerase chain reaction in peroperative period von Ge, Ming Jian, Shi, De, Wu, Qing Chen, Wang, Mei, Li, Liang Bin

“… To assess whether surgical manoeuvre or resection of lung cancer could lead to haematogenous dissemination of malignant cells. In the mean time, the …”
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Loss of Junctional Adhesion Molecule A Promotes Severe Steatohepatitis in Mice on a Diet High in Saturated Fat, Fructose, and Cholesterol von Rahman, Khalidur, Desai, Chirayu, Iyer, Smita S, Thorn, Natalie E, Kumar, Pradeep, Liu, Yunshan, Smith, Tekla, Neish, Andrew S, Li, Hongliang, Tan, Shiyun, Wu, Pengbo, Liu, Xiaoxiong, Yu, Yuanjie, Farris, Alton B, Nusrat, Asma, Parkos, Charles A, Anania, Frank A

“… There is evidence from clinical studies that compromised intestinal epithelial permeability contributes to the development of nonalcoholic steatohepatitis …”
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