Suchergebnisse - "O'Reilly, Francis J"

Cross-linking mass spectrometry: methods and applications in structural, molecular and systems biology von O'Reilly, Francis J, Rappsilber, Juri

“… Over the past decade, cross-linking mass spectrometry (CLMS) has developed into a robust and flexible tool that provides medium-resolution structural …”
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Reliable identification of protein-protein interactions by crosslinking mass spectrometry von Lenz, Swantje, Sinn, Ludwig R., O’Reilly, Francis J., Fischer, Lutz, Wegner, Fritz, Rappsilber, Juri

“… Protein-protein interactions govern most cellular pathways and processes, and multiple technologies have emerged to systematically map them. Assessing the …”
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Cross-linking mass spectrometry for mapping protein complex topologies in situ von Lee, Kitaik, O'Reilly, Francis J

“… Cross-linking mass spectrometry has become an established technology to provide structural information on the topology and dynamics of protein complexes …”
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A folded conformation of MukBEF and cohesin von Bürmann, Frank, Lee, Byung-Gil, Than, Thane, Sinn, Ludwig, O'Reilly, Francis J, Yatskevich, Stanislau, Rappsilber, Juri, Hu, Bin, Nasmyth, Kim, Löwe, Jan

“… Structural maintenance of chromosomes (SMC)-kleisin complexes organize chromosomal DNAs in all domains of life, with key roles in chromosome segregation, DNA …”
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In-cell architecture of an actively transcribing-translating expressome von O'Reilly, Francis J, Xue, Liang, Graziadei, Andrea, Sinn, Ludwig, Lenz, Swantje, Tegunov, Dimitry, Blötz, Cedric, Singh, Neil, Hagen, Wim J H, Cramer, Patrick, Stülke, Jörg, Mahamid, Julia, Rappsilber, Juri

“… Structural biology studies performed inside cells can capture molecular machines in action within their native context. In this work, we developed an …”
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Cryo‐EM reveals the complex architecture of dynactin's shoulder region and pointed end von Lau, Clinton K, O’Reilly, Francis J, Santhanam, Balaji, Lacey, Samuel E, Rappsilber, Juri, Carter, Andrew P

“… Dynactin is a 1.1 MDa complex that activates the molecular motor dynein for ultra‐processive transport along microtubules. In order to do this, it forms a …”
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mRNA recognition and packaging by the human transcription–export complex von Pacheco-Fiallos, Belén, Vorländer, Matthias K., Riabov-Bassat, Daria, Fin, Laura, O’Reilly, Francis J., Ayala, Farja I., Schellhaas, Ulla, Rappsilber, Juri, Plaschka, Clemens

“… Newly made mRNAs are processed and packaged into mature ribonucleoprotein complexes (mRNPs) and are recognized by the essential transcription–export complex …”
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Cryo-EM structures of holo condensin reveal a subunit flip-flop mechanism von Lee, Byung-Gil, Merkel, Fabian, Allegretti, Matteo, Hassler, Markus, Cawood, Christopher, Lecomte, Léa, O'Reilly, Francis J, Sinn, Ludwig R, Gutierrez-Escribano, Pilar, Kschonsak, Marc, Bravo, Sol, Nakane, Takanori, Rappsilber, Juri, Aragon, Luis, Beck, Martin, Löwe, Jan, Haering, Christian H

“… Complexes containing a pair of structural maintenance of chromosomes (SMC) family proteins are fundamental for the three-dimensional (3D) organization of …”
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Protein complexes in cells by AI‐assisted structural proteomics von O'Reilly, Francis J, Graziadei, Andrea, Forbrig, Christian, Bremenkamp, Rica, Charles, Kristine, Lenz, Swantje, Elfmann, Christoph, Fischer, Lutz, Stülke, Jörg, Rappsilber, Juri

“… Accurately modeling the structures of proteins and their complexes using artificial intelligence is revolutionizing molecular biology. Experimental data enable …”
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Specific recognition and ubiquitination of translating ribosomes by mammalian CCR4–NOT von Absmeier, Eva, Chandrasekaran, Viswanathan, O’Reilly, Francis J., Stowell, James A. W., Rappsilber, Juri, Passmore, Lori A.

“… Translation affects messenger RNA stability and, in yeast, this is mediated by the Ccr4–Not deadenylation complex. The details of this process in mammals …”
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A Structure-Based Mechanism for DNA Entry into the Cohesin Ring von Higashi, Torahiko L, Eickhoff, Patrik, Sousa, Joana S, Locke, Julia, Nans, Andrea, Flynn, Helen R, Snijders, Ambrosius P, Papageorgiou, George, O'Reilly, Nicola, Chen, Zhuo A, O'Reilly, Francis J, Rappsilber, Juri, Costa, Alessandro, Uhlmann, Frank

“… Despite key roles in sister chromatid cohesion and chromosome organization, the mechanism by which cohesin rings are loaded onto DNA is still unknown. Here we …”
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Molecular mechanism of IKK catalytic dimer docking to NF-κB substrates von Li, Changqing, Moro, Stefano, Shostak, Kateryna, O’Reilly, Francis J., Donzeau, Mariel, Graziadei, Andrea, McEwen, Alastair G., Desplancq, Dominique, Poussin-Courmontagne, Pierre, Bachelart, Thomas, Fiskin, Mert, Berrodier, Nicolas, Pichard, Simon, Brillet, Karl, Orfanoudakis, Georges, Poterszman, Arnaud, Torbeev, Vladimir, Rappsilber, Juri, Davey, Norman E., Chariot, Alain, Zanier, Katia

“… The inhibitor of κB (IκB) kinase (IKK) is a central regulator of NF-κB signaling. All IKK complexes contain hetero- or homodimers of the catalytic IKKβ and/or …”
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Improved Peptide Backbone Fragmentation Is the Primary Advantage of MS-Cleavable Crosslinkers von Kolbowski, Lars, Lenz, Swantje, Fischer, Lutz, Sinn, Ludwig R, O'Reilly, Francis J, Rappsilber, Juri

“… Proteome-wide crosslinking mass spectrometry studies have coincided with the advent of mass spectrometry (MS)-cleavable crosslinkers that can reveal the …”
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The structure of human thyroglobulin von Coscia, Francesca, Taler-Verčič, Ajda, Chang, Veronica T., Sinn, Ludwig, O’Reilly, Francis J., Izoré, Thierry, Renko, Miha, Berger, Imre, Rappsilber, Juri, Turk, Dušan, Löwe, Jan

“… Thyroglobulin (TG) is the protein precursor of thyroid hormones, which are essential for growth, development and the control of metabolism in vertebrates 1 , 2 …”
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Purified Smc5/6 Complex Exhibits DNA Substrate Recognition and Compaction von Gutierrez-Escribano, Pilar, Hormeño, Silvia, Madariaga-Marcos, Julene, Solé-Soler, Roger, O'Reilly, Francis J, Morris, Kyle, Aicart-Ramos, Clara, Aramayo, Ricardo, Montoya, Alex, Kramer, Holger, Rappsilber, Juri, Torres-Rosell, Jordi, Moreno-Herrero, Fernando, Aragon, Luis

“… Eukaryotic SMC complexes, cohesin, condensin, and Smc5/6, use ATP hydrolysis to power a plethora of functions requiring organization and restructuring of …”
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In Situ Structural Restraints from Cross-Linking Mass Spectrometry in Human Mitochondria von Ryl, Petra S J, Bohlke-Schneider, Michael, Lenz, Swantje, Fischer, Lutz, Budzinski, Lisa, Stuiver, Marchel, Mendes, Marta M L, Sinn, Ludwig, O'Reilly, Francis J, Rappsilber, Juri

“… The field of structural biology is increasingly focusing on studying proteins in situ, i.e., in their greater biological context. Cross-linking mass …”
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Structure of apolipoprotein B100 bound to the low-density lipoprotein receptor von Reimund, Mart, Dearborn, Altaira D., Graziano, Giorgio, Lei, Haotian, Ciancone, Anthony M., Kumar, Ashish, Holewinski, Ronald, Neufeld, Edward B., O’Reilly, Francis J., Remaley, Alan T., Marcotrigiano, Joseph

“… Apolipoprotein B100 (apoB100) is a structural component of low-density lipoprotein (LDL) and a ligand for the LDL receptor (LDLR) 1 . Mutations in apoB100 or …”
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An integrated workflow for crosslinking mass spectrometry von Mendes, Marta L, Fischer, Lutz, Chen, Zhuo A, Barbon, Marta, O'Reilly, Francis J, Giese, Sven H, Bohlke‐Schneider, Michael, Belsom, Adam, Dau, Therese, Combe, Colin W, Graham, Martin, Eisele, Markus R, Baumeister, Wolfgang, Speck, Christian, Rappsilber, Juri

“… We present a concise workflow to enhance the mass spectrometric detection of crosslinked peptides by introducing sequential digestion and the crosslink …”
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Integrative structure of a 10-megadalton eukaryotic pyruvate dehydrogenase complex from native cell extracts von Kyrilis, Fotis L., Semchonok, Dmitry A., Skalidis, Ioannis, Tüting, Christian, Hamdi, Farzad, O’Reilly, Francis J., Rappsilber, Juri, Kastritis, Panagiotis L.

“… The pyruvate dehydrogenase complex (PDHc) is a giant enzymatic assembly involved in pyruvate oxidation. PDHc components have been characterized in isolation, …”
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M2 pyruvate kinase provides a mechanism for nutrient sensing and regulation of cell proliferation von Morgan, Hugh P, O'Reilly, Francis J, Wear, Martin A, O'Neill, J Robert, Fothergill-Gilmore, Linda A, Hupp, Ted, Walkinshaw, Malcolm D

“… We show that the M2 isoform of pyruvate kinase (M2PYK) exists in equilibrium between monomers and tetramers regulated by allosteric binding of naturally …”
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