Suchergebnisse - "Nureki, Osamu"

Cryo-EM structures capture the transport cycle of the P4-ATPase flippase von Hiraizumi, Masahiro, Yamashita, Keitaro, Nishizawa, Tomohiro, Nureki, Osamu

“… In eukaryotic membranes, type IV P-type adenosine triphosphatases (P4-ATPases) mediate the translocation of phospholipids from the outer to the inner leaflet …”
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Cyclic GMP-AMP as an Endogenous Second Messenger in Innate Immune Signaling by Cytosolic DNA von Kato, Kazuki, Omura, Hiroki, Ishitani, Ryuichiro, Nureki, Osamu

“… The innate immune system functions as the first line of defense against invading bacteria and viruses. In this context, the cGAS/STING [cyclic guanosine …”
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Structural biology of the multidrug and toxic compound extrusion superfamily transporters von Kusakizako, Tsukasa, Miyauchi, Hirotake, Ishitani, Ryuichiro, Nureki, Osamu

“… Xenobiotic and metabolite extrusion is an important process for the proper functions of cells and their compartments, including acidic organelles. MATE …”
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Taste transduction and channel synapses in taste buds von Taruno, Akiyuki, Nomura, Kengo, Kusakizako, Tsukasa, Ma, Zhongming, Nureki, Osamu, Foskett, J. Kevin

“… The variety of taste sensations, including sweet, umami, bitter, sour, and salty, arises from diverse taste cells, each of which expresses specific taste …”
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Structures and mechanisms of CRISPR RNA-guided effector nucleases von Nishimasu, Hiroshi, Nureki, Osamu

“… [Display omitted] •Incredible structural and mechanistic diversity in the CRISPR-Cas effector nucleases.•Divergent evolution of the type I (Cascade-Cas3) and …”
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Cryo-EM advances in GPCR structure determination von Shihoya, Wataru, Iwama, Aika, Sano, Fumiya K, Nureki, Osamu

“… G-protein-coupled receptors (GPCRs) constitute a prominent superfamily in humans, and are categorized into six classes (A through F) that play indispensable …”
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Structural Basis for the Altered PAM Specificities of Engineered CRISPR-Cas9 von Hirano, Seiichi, Nishimasu, Hiroshi, Ishitani, Ryuichiro, Nureki, Osamu

“… The RNA-guided endonuclease Cas9 cleaves double-stranded DNA targets bearing a PAM (protospacer adjacent motif) and complementarity to the guide RNA. A recent …”
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Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1 von Yamano, Takashi, Zetsche, Bernd, Ishitani, Ryuichiro, Zhang, Feng, Nishimasu, Hiroshi, Nureki, Osamu

“… The RNA-guided Cpf1 (also known as Cas12a) nuclease associates with a CRISPR RNA (crRNA) and cleaves the double-stranded DNA target complementary to the crRNA …”
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Structural insights into tetraspanin CD9 function von Umeda, Rie, Satouh, Yuhkoh, Takemoto, Mizuki, Nakada-Nakura, Yoshiko, Liu, Kehong, Yokoyama, Takeshi, Shirouzu, Mikako, Iwata, So, Nomura, Norimichi, Sato, Ken, Ikawa, Masahito, Nishizawa, Tomohiro, Nureki, Osamu

“… Tetraspanins play critical roles in various physiological processes, ranging from cell adhesion to virus infection. The members of the tetraspanin family have …”
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Molecular mechanisms of building blocks of life towards medicinal applications von Nureki, Osamu

“… Membrane and membrane-spanning proteins, and non-coding RNA are biomolecules to play central roles in beginning of life and distinguishing higher-order …”
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Engineered CRISPR-Cas9 nuclease with expanded targeting space von Nishimasu, Hiroshi, Shi, Xi, Ishiguro, Soh, Gao, Linyi, Hirano, Seiichi, Okazaki, Sae, Noda, Taichi, Abudayyeh, Omar O, Gootenberg, Jonathan S, Mori, Hideto, Oura, Seiya, Holmes, Benjamin, Tanaka, Mamoru, Seki, Motoaki, Hirano, Hisato, Aburatani, Hiroyuki, Ishitani, Ryuichiro, Ikawa, Masahito, Yachie, Nozomu, Zhang, Feng, Nureki, Osamu

“… The RNA-guided endonuclease Cas9 cleaves its target DNA and is a powerful genome-editing tool. However, the widely used Cas9 enzyme (SpCas9) requires an NGG …”
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Structural Insights into Endothelin Receptor Signaling von Shihoya, Wataru, Sano, Fumiya K, Nureki, Osamu

“… Endothelins and their receptors, ETA and ETB, modulate vital cellular processes, including growth, survival, invasion, and angiogenesis, through multiple G …”
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TMC1 and TMC2 Proteins Are Pore-Forming Subunits of Mechanosensitive Ion Channels von Jia, Yanyan, Zhao, Yimeng, Kusakizako, Tsukasa, Wang, Yao, Pan, Chengfang, Zhang, Yuwei, Nureki, Osamu, Hattori, Motoyuki, Yan, Zhiqiang

“… Transmembrane channel-like (TMC) 1 and 2 are required for the mechanotransduction of mouse inner ear hair cells and localize to the site of mechanotransduction …”
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The structure of lipid nanodisc-reconstituted TRPV3 reveals the gating mechanism von Shimada, Hiroto, Kusakizako, Tsukasa, Dung Nguyen, T H, Nishizawa, Tomohiro, Hino, Tomoya, Tominaga, Makoto, Nureki, Osamu

“… Transient receptor potential vanilloid subfamily member 3 (TRPV3) is a temperature-sensitive cation channel. Previous cryo-EM analyses of TRPV3 in detergent …”
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Structural insights into cGAMP degradation by Ecto-nucleotide pyrophosphatase phosphodiesterase 1 von Kato, Kazuki, Nishimasu, Hiroshi, Oikawa, Daisuke, Hirano, Seiichi, Hirano, Hisato, Kasuya, Go, Ishitani, Ryuichiro, Tokunaga, Fuminori, Nureki, Osamu

“… ENPP1 (Ecto-nucleotide pyrophosphatase phosphodiesterase 1), a type II transmembrane glycoprotein, hydrolyzes ATP to produce AMP and diphosphate, thereby …”
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Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex von Konermann, Silvana, Brigham, Mark D., Trevino, Alexandro E., Joung, Julia, Abudayyeh, Omar O., Barcena, Clea, Hsu, Patrick D., Habib, Naomi, Gootenberg, Jonathan S., Nishimasu, Hiroshi, Nureki, Osamu, Zhang, Feng

“… Systematic interrogation of gene function requires the ability to perturb gene expression in a robust and generalizable manner. Here we describe …”
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Recent Advances in the Structural Biology of the Volume-Regulated Anion Channel LRRC8 von Kasuya, Go, Nureki, Osamu

“… Members of the leucine-rich repeat-containing 8 (LRRC8) protein family, composed of five LRRC8A-E isoforms, are pore-forming components of the volume-regulated …”
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Structure of the miniature type V-F CRISPR-Cas effector enzyme von Takeda, Satoru N, Nakagawa, Ryoya, Okazaki, Sae, Hirano, Hisato, Kobayashi, Kan, Kusakizako, Tsukasa, Nishizawa, Tomohiro, Yamashita, Keitaro, Nishimasu, Hiroshi, Nureki, Osamu

“… RNA-guided DNA endonucleases derived from CRISPR-Cas adaptive immune systems are widely used as powerful genome-engineering tools. Among the diverse CRISPR-Cas …”
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Structure of the Dicer-2–R2D2 heterodimer bound to a small RNA duplex von Yamaguchi, Sonomi, Naganuma, Masahiro, Nishizawa, Tomohiro, Kusakizako, Tsukasa, Tomari, Yukihide, Nishimasu, Hiroshi, Nureki, Osamu

“… In flies, Argonaute2 (Ago2) and small interfering RNA (siRNA) form an RNA-induced silencing complex to repress viral transcripts 1 . The RNase III enzyme …”
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Base editors for simultaneous introduction of C-to-T and A-to-G mutations von Sakata, Rina C, Ishiguro, Soh, Mori, Hideto, Tanaka, Mamoru, Tatsuno, Kenji, Ueda, Hiroki, Yamamoto, Shogo, Seki, Motoaki, Masuyama, Nanami, Nishida, Keiji, Nishimasu, Hiroshi, Arakawa, Kazuharu, Kondo, Akihiko, Nureki, Osamu, Tomita, Masaru, Aburatani, Hiroyuki, Yachie, Nozomu

“… We describe base editors that combine both cytosine and adenine base-editing functions. A codon-optimized fusion of the cytosine deaminase PmCDA1, the …”
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