Search Results - "Next-generation sequencing"

Next-Generation Sequencing: From Basic Research to Diagnostics by Voelkerding, Karl V, Dames, Shale A, Durtschi, Jacob D

“… The commercial launch of the first massively parallel pyrosequencing platform in 2005 ushered in the new era of high-throughput genomic analysis now referred to as next-generation sequencing (NGS). Content…”
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Clinical validation of a next-generation sequencing-based multi-cancer early detection “liquid biopsy” blood test in over 1,000 dogs using an independent testing set: The CANcer Detection in Dogs (CANDiD) study by Flory, Andi, Kruglyak, Kristina M., Tynan, John A., McLennan, Lisa M., Rafalko, Jill M., Fiaux, Patrick Christian, Hernandez, Gilberto E., Marass, Francesco, Nakashe, Prachi, Ruiz-Perez, Carlos A., Fath, Donna M., Jennings, Thuy, Motalli-Pepio, Rita, Wotrang, Kate, McCleary-Wheeler, Angela L., Lana, Susan, Phillips, Brenda, Flesner, Brian K., Leibman, Nicole F., LaDue, Tracy, Tripp, Chelsea D., Coomber, Brenda L., Woods, J. Paul, Miller, Mairin, Aiken, Sean W., Wolf-Ringwall, Amber, Borgatti, Antonella, Kraska, Kathleen, Thomson, Christopher B., Kosanovich Cahalane, Alane, Murray, Rebecca L., Kisseberth, William C., Camps-Palau, Maria A., Floch, Franck, Beaudu-Lange, Claire, Klajer-Peres, Aurélia, Keravel, Olivier, Fribourg-Blanc, Luc-André, Mazetier, Pascale Chicha, Marco, Angelo, McLeod, Molly B., Portillo, Erin, Clark, Terry S., Judd, Scott, Feinberg, C. Kirk, Benitez, Marie, Runyan, Candace, Hackett, Lindsey, Lafey, Scott, Richardson, Danielle, Vineyard, Sarah, Tefend Campbell, Mary, Dharajiya, Nilesh, Jensen, Taylor J., van den Boom, Dirk, Diaz, Luis A., Grosu, Daniel S., Polk, Arthur, Marsal, Kalle, Hicks, Susan Cho, Lytle, Katherine M., Holtvoigt, Lauren, Chibuk, Jason, Chorny, Ilya, Tsui, Dana W. Y.

“…” test developed for noninvasive detection and characterization of cancer in dogs using next-generation sequencing (NGS…”
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Plasma Microbial Cell-free DNA Next-generation Sequencing in the Diagnosis and Management of Febrile Neutropenia by Benamu, Esther, Gajurel, Kiran, Anderson, Jill N, Lieb, Tullia, Gomez, Carlos A, Seng, Hon, Aquino, Romielle, Hollemon, Desiree, Hong, David K, Blauwkamp, Timothy A, Kertesz, Mickey, Blair, Lily, Bollyky, Paul L, Medeiros, Bruno C, Coutre, Steven, Zompi, Simona, Montoya, Jose G, Deresinski, Stan

“… Up to 5 blood samples were collected per subject within 24 hours of fever onset (T1) and every 2 to 3 days…”
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Rapid Targeted Next-Generation Sequencing Platform for Molecular Screening and Clinical Genotyping in Subjects with Hemoglobinopathies by Shang, Xuan, Peng, Zhiyu, Ye, Yuhua, Asan, Zhang, Xinhua, Chen, Yan, Zhu, Baosheng, Cai, Wangwei, Chen, Shaoke, Cai, Ren, Guo, Xiaoling, Zhang, Chonglin, Zhou, Yuqiu, Huang, Shuodan, Liu, Yanhui, Chen, Biyan, Yan, Shanhuo, Chen, Yajun, Ding, Hongmei, Yin, Xiaolin, Wu, Liusong, He, Jing, Huang, Dongai, He, Sheng, Yan, Tizhen, Fan, Xin, Zhou, Yuehong, Wei, Xiaofeng, Zhao, Sumin, Cai, Decheng, Guo, Fengyu, Zhang, Qianqian, Li, Yun, Zhang, Xuelian, Lu, Haorong, Huang, Huajie, Guo, Junfu, Zhu, Fei, Yuan, Yuan, Zhang, Li, Liu, Na, Li, Zhiming, Jiang, Hui, Zhang, Qiang, Zhang, Yijia, Juhari, Wan Khairunnisa Wan, Hanafi, Sarifah, Zhou, Wanjun, Xiong, Fu, Yang, Huanming, Wang, Jian, Zilfalil, Bin Alwi, Qi, Ming, Yang, Yaping, Yin, Ye, Mao, Mao, Xu, Xiangmin

“… A comprehensive next-generation sequencing (NGS) test would greatly facilitate screening and diagnosis of these disorders…”
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Next Generation Sequencing of Pooled Samples: Guideline for Variants’ Filtering by Anand, Santosh, Mangano, Eleonora, Barizzone, Nadia, Bordoni, Roberta, Sorosina, Melissa, Clarelli, Ferdinando, Corrado, Lucia, Martinelli Boneschi, Filippo, D’Alfonso, Sandra, De Bellis, Gianluca

“…Sequencing large number of individuals, which is often needed for population genetics studies, is still economically challenging despite falling costs of Next Generation Sequencing (NGS…”
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Targeted next-generation sequencing (NGS) analysis of mutations in nonsyndromic tooth agenesis candidate genes: Analysis of a Turkish cohort by Keskin, Gül, Karaer, Kadri, Uçar Gündoğar, Zübeyde

“…Purpose The goal of this study was to assess genes known to be associated with tooth agenesis with next-generation sequencing (NGS…”
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Allele and haplotype frequencies of 11 HLA loci in Koreans by next‐generation sequencing by Jung, Kiwook, Kim, Jisoo G., Shin, Sue, Roh, Eun Youn, Hong, Yun Ji, Song, Eun Young

“…‐generation sequencing (NGS)‐based HLA typing. A total of 339 samples from unrelated healthy subjects were genotyped for HLA‐A, ‐B, ‐C, ‐DRB1, ‐DRB3, ‐DRB4, ‐DRB5, ‐DQB1, ‐DQA1, ‐DPB1…”
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Next‐generation sequencing‐defined minimal residual disease before stem cell transplantation predicts acute myeloid leukemia relapse by Press, Richard D., Eickelberg, Garrett, Froman, Allison, Yang, Fei, Stentz, Alex, Flatley, Ellen M., Fan, Guang, Lim, Jeong Y., Meyers, Gabrielle, Maziarz, Richard T., Cook, Rachel J.

“…‐generation sequencing (NGS)‐based MRD detection undertaken before hematopoietic stem cell transplantation (HSCT). Forty…”
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Application of metagenomic next-generation sequencing and targeted metagenomic next-generation sequencing in diagnosing pulmonary infections in immunocompetent and immunocompromised patients by Liu, Yong, Wu, Wencai, Xiao, Yunping, Zou, Hongyan, Hao, Sijia, Jiang, Yanfang

“…Metagenomic next-generation sequencing (mNGS) technology has been widely used to diagnose various infections…”
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Computational methods for discovering structural variation with next-generation sequencing by Medvedev, Paul, Stanciu, Monica, Brudno, Michael

“… With the advent of next-generation sequencing, a new generation of methods is being developed to tackle the challenges of short reads, while taking advantage of the high coverage the new sequencing technologies provide…”
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Rapid 16S rRNA Next-Generation Sequencing of Polymicrobial Clinical Samples for Diagnosis of Complex Bacterial Infections by Salipante, Stephen J., Sengupta, Dhruba J., Rosenthal, Christopher, Costa, Gina, Spangler, Jessica, Sims, Elizabeth H., Jacobs, Michael A., Miller, Samuel I., Hoogestraat, Daniel R., Cookson, Brad T., McCoy, Connor, Matsen, Frederick A., Shendure, Jay, Lee, Clarence C., Harkins, Timothy T., Hoffman, Noah G.

“…Classifying individual bacterial species comprising complex, polymicrobial patient specimens remains a challenge for culture-based and molecular microbiology…”
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Computational tools for copy number variation (CNV) detection using next-generation sequencing data: features and perspectives by Zhao, Min, Wang, Qingguo, Wang, Quan, Jia, Peilin, Zhao, Zhongming

“… changes in genomes until most recently high-resolution sequence data can be analyzed by next-generation sequencing (NGS…”
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Comparison of the gut microbiota composition between obese and non-obese individuals in a Japanese population, as analyzed by terminal restriction fragment length polymorphism and next-generation sequencing by Kasai, Chika, Sugimoto, Kazushi, Moritani, Isao, Tanaka, Junichiro, Oya, Yumi, Inoue, Hidekazu, Tameda, Masahiko, Shiraki, Katsuya, Ito, Masaaki, Takei, Yoshiyuki, Takase, Kojiro

“…) analysis, samples from 10 subjects (4 non-obese and 6 obese) were selected and subjected to next-generation sequencing for species-level…”
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The spectrum of BRCA mutations and characteristics of BRCA‐associated breast cancers in China: Screening of 2,991 patients and 1,043 controls by next‐generation sequencing by Lang, Guan‐Tian, Shi, Jin‐Xiu, Hu, Xin, Zhang, Chen‐Hui, Shan, Ling, Song, Chuan‐Gui, Zhuang, Zhi‐Gang, Cao, A‐Yong, Ling, Hong, Yu, Ke‐Da, Li, Shan, Sun, Meng‐Hong, Zhou, Xiao‐Yan, Huang, Wei, Shao, Zhi‐Ming

“…‐generation sequencing (NGS). A total of 4,034 Chinese subjects were screened for germline BRCA1/2 mutations, including 2,991 breast cancer patients and 1,043 healthy individuals from the community enrolled as controls…”
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Using next‐generation sequencing to detect oral microbiome change following periodontal interventions: A systematic review by Zhang, Ya, Qi, Yinliang, Lo, Edward C. M., McGrath, Colman, Mei, May Lei, Dai, Ruoxi

“…‐generation sequencing (NGS)‐metagenomic analysis following periodontal interventions among systematically healthy subjects…”
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Targeted next generation sequencing as a tool for precision medicine by Gulilat, Markus, Lamb, Tyler, Teft, Wendy A., Wang, Jian, Dron, Jacqueline S., Robinson, John F., Tirona, Rommel G., Hegele, Robert A., Kim, Richard B., Schwarz, Ute I.

“…Background Targeted next-generation sequencing (NGS) enables rapid identification of common and rare genetic variation…”
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Next-Generation Sequencing Reveals That HLA-DRB3, -DRB4, and -DRB5 May Be Associated With Islet Autoantibodies and Risk for Childhood Type 1 Diabetes by Zhao, Lue Ping, Alshiekh, Shehab, Zhao, Michael, Carlsson, Annelie, Larsson, Helena Elding, Forsander, Gun, Ivarsson, Sten A, Ludvigsson, Johnny, Kockum, Ingrid, Marcus, Claude, Persson, Martina, Samuelsson, Ulf, Örtqvist, Eva, Pyo, Chul-Woo, Nelson, Wyatt C, Geraghty, Daniel E, Lernmark, Åke

“…) at clinical diagnosis is unclear. Next-generation sequencing (NGS) was used to determine all DRB alleles in consecutively diagnosed patients ages 1-18 years with islet autoantibody-positive type 1 diabetes (n = 970…”
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Genetic diagnosis of familial hypercholesterolaemia by targeted next‐generation sequencing by Maglio, C., Mancina, R. M., Motta, B. M., Stef, M., Pirazzi, C., Palacios, L., Askaryar, N., Borén, J., Wiklund, O., Romeo, S.

“…Objectives The aim of this study was to combine clinical criteria and next‐generation sequencing (pyrosequencing…”
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Single-Cell Applications of Next-Generation Sequencing by Anaparthy, Naishitha, Ho, Yu-Jui, Martelotto, Luciano, Hammell, Molly, Hicks, James

“…s, and even cancer, makes them the subject of intense study. Next-generation sequencing (NGS) of RNA and DNA has opened a new frontier of (single)-cell biology…”
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Next‐generation sequencing improves molecular epidemiological characterization of thalassemia in Chenzhou Region, P.R. China by Zhang, Haoqing, Li, Caiyun, Li, Jianbiao, Hou, Shuai, Chen, Danjing, Yan, Haiying, Chen, Shiping, Liu, Saijun, Yin, Zhenzhen, Yang, Xiaoqin, Tan, Jufang, Huang, Xiaoyan, Zhang, Liming, Fang, Junbin, Zhang, Caifen, Li, Wei, Guo, Jian, Lei, Dongzhu

“…‐generation sequencing (NGS) and Gap‐PCR. Results In total, 1704 subjects were diagnosed as thalassemia carriers with a total prevalence rate of 10.78%, including 943 α…”
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