Search Results - "Mathis, Nicolas"

Enhancing prime editor activity by directed protein evolution in yeast by Weber, Yanik, Böck, Desirée, Ivașcu, Anastasia, Mathis, Nicolas, Rothgangl, Tanja, Ioannidi, Eleonora I., Blaudt, Alex C., Tidecks, Lisa, Vadovics, Máté, Muramatsu, Hiromi, Reichmuth, Andreas, Marquart, Kim F., Kissling, Lucas, Pardi, Norbert, Jinek, Martin, Schwank, Gerald

“…Prime editing is a highly versatile genome editing technology that enables the introduction of base substitutions, insertions, and deletions. However, compared…”
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A paracrine activin A–mDia2 axis promotes squamous carcinogenesis via fibroblast reprogramming by Cangkrama, Michael, Wietecha, Mateusz, Mathis, Nicolas, Okumura, Rin, Ferrarese, Luca, Al‐Nuaimi, Dunja, Antsiferova, Maria, Dummer, Reinhard, Innocenti, Metello, Werner, Sabine

“…Cancer‐associated fibroblasts (CAFs) are key regulators of tumorigenesis and promising targets for next‐generation therapies. We discovered that cancer…”
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Predicting adenine base editing efficiencies in different cellular contexts by deep learning by Kissling, Lucas, Mollaysa, Amina, Janjuha, Sharan, Mathis, Nicolas, Marquart, Kim F., Weber, Yanik, Moon, Woohyun J., Lin, Paulo J. C., Fan, Steven H. Y., Muramatsu, Hiromi, Vadovics, Máté, Allam, Ahmed, Pardi, Norbert, Tam, Ying K., Krauthammer, Michael, Schwank, Gerald

“…Background Adenine base editors (ABEs) enable the conversion of A•T to G•C base pairs. Since the sequence of the target locus influences base editing…”
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Replacing the SpCas9 HNH domain by deaminases generates compact base editors with an alternative targeting scope by Villiger, Lukas, Schmidheini, Lukas, Mathis, Nicolas, Rothgangl, Tanja, Marquart, Kim, Schwank, Gerald

“…Base editors are RNA-guided deaminases that enable site-specific nucleotide transitions. The targeting scope of these Cas-deaminase fusion proteins critically…”
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Predicting prime editing efficiency and product purity by deep learning by Mathis, Nicolas, Allam, Ahmed, Kissling, Lucas, Marquart, Kim Fabiano, Schmidheini, Lukas, Solari, Cristina, Balázs, Zsolt, Krauthammer, Michael, Schwank, Gerald

“…Prime editing is a versatile genome editing tool but requires experimental optimization of the prime editing guide RNA (pegRNA) to achieve high editing…”
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In vivo prime editing of a metabolic liver disease in mice by Böck, Desirée, Rothgangl, Tanja, Villiger, Lukas, Schmidheini, Lukas, Matsushita, Mai, Mathis, Nicolas, Ioannidi, Eleonora, Rimann, Nicole, Grisch-Chan, Hiu Man, Kreutzer, Susanne, Kontarakis, Zacharias, Kopf, Manfred, Thöny, Beat, Schwank, Gerald

“…Prime editing is a highly versatile CRISPR-based genome editing technology that works without DNA double-strand break formation. Despite rapid technological…”
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Systematic pegRNA design with PRIDICT2.0 and ePRIDICT for efficient prime editing by Mathis, Nicolas, Marquart, Kim Fabiano, Allam, Ahmed, Krauthammer, Michael, Schwank, Gerald

“…Prime editing is a versatile genome editing technology that enables precise genetic modifications without inducing DNA double-strand breaks. Owing to numerous…”
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Continuous directed evolution of a compact CjCas9 variant with broad PAM compatibility by Schmidheini, Lukas, Mathis, Nicolas, Marquart, Kim Fabiano, Rothgangl, Tanja, Kissling, Lucas, Böck, Desirée, Chanez, Christelle, Wang, Jingrui Priscilla, Jinek, Martin, Schwank, Gerald

“…CRISPR–Cas9 genome engineering is a powerful technology for correcting genetic diseases. However, the targeting range of Cas9 proteins is limited by their…”
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Machine learning prediction of prime editing efficiency across diverse chromatin contexts by Mathis, Nicolas, Allam, Ahmed, Tálas, András, Kissling, Lucas, Benvenuto, Elena, Schmidheini, Lukas, Schep, Ruben, Damodharan, Tanav, Balázs, Zsolt, Janjuha, Sharan, Ioannidi, Eleonora I, Böck, Desirée, van Steensel, Bas, Krauthammer, Michael, Schwank, Gerald

“…The success of prime editing depends on the prime editing guide RNA (pegRNA) design and target locus. Here, we developed machine learning models that reliably…”
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Publisher Correction: Machine learning prediction of prime editing efficiency across diverse chromatin contexts by Mathis, Nicolas, Allam, Ahmed, Tálas, András, Kissling, Lucas, Benvenuto, Elena, Schmidheini, Lukas, Schep, Ruben, Damodharan, Tanav, Balázs, Zsolt, Janjuha, Sharan, Ioannidi, Eleonora I, Böck, Desirée, van Steensel, Bas, Krauthammer, Michael, Schwank, Gerald

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Effective genome editing with an enhanced ISDra2 TnpB system and deep learning-predicted ωRNAs by Marquart, Kim Fabiano, Mathis, Nicolas, Mollaysa, Amina, Müller, Saphira, Kissling, Lucas, Rothgangl, Tanja, Schmidheini, Lukas, Kulcsár, Péter István, Allam, Ahmed, Kaufmann, Masako M., Matsushita, Mai, Haenggi, Tatjana, Cathomen, Toni, Kopf, Manfred, Krauthammer, Michael, Schwank, Gerald

“…Transposon (IS200/IS605)-encoded TnpB proteins are predecessors of class 2 type V CRISPR effectors and have emerged as one of the most compact genome editors…”
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Effective Genome Editing with ISDra2 TnpB and Deep Learning-Predicted ωRNAs by Marquart, Kim Fabiano, Mathis, Nicolas, Mollaysa, Amina, Müller, Saphira, Kissling, Lucas, Rothgangl, Tanja, Schmidheini, Lukas, Kulcsár, Péter István, Allam, Ahmed, Kaufmann, Masako M., Matsushita, Mai, Haenggi, Tatjana, Cathomen, Toni, Kopf, Manfred, Krauthammer, Michael, Schwank, Gerald

“…Transposon (IS200/IS605)-encoded TnpB proteins are predecessors of class 2 type V CRISPR effectors and have emerged as one of the most compact genome editors…”
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Stress-triggered hematopoietic stem cell proliferation relies on PrimPol-mediated repriming by Jacobs, Kurt, Doerdelmann, Cyril, Krietsch, Jana, González-Acosta, Daniel, Mathis, Nicolas, Kushinsky, Saul, Guarino, Estrella, Gómez-Escolar, Carmen, Martinez, Dolores, Schmid, Jonas A, Leary, Peter J, Freire, Raimundo, Ramiro, Almudena R, Eischen, Christine M, Mendez, Juan, Lopes, Massimo

“…Stem cell division is linked to tumorigenesis by yet-elusive mechanisms. The hematopoietic system reacts to stress by triggering hematopoietic stem and…”
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Boosting CAR T-Cell Efficacy by Blocking Proteasomal Degradation of Membrane Antigens by Rieger, Leonie, Irlinger, Kilian, Füchsl, Franziska, Tietje, Marlene, Purcarea, Anna, Barbian, Nicolas Mathis, Faber, Melanie, Vogelsang, Carolin, Pfeuffer, Lisa, Stotz, Sonja, Karpiuk, Oleksandra, Schulze, Tobias, Augsburger, Abirami, Glaisner, Nadine, Konetzki, Verena, Friedel, Sabrina, Besse, Andrej, Besse, Lenka, Driessen, Christoph, Buchner, Maike, Schwamborn, Kristina, Steiger, Katja, Giansanti, Piero, Theurich, Sebastian, Waldschmidt, Johannes M, Kortüm, Klaus Martin, Hudecek, Michael, Einsele, Hermann, Högner, Marion, Kuster, Bernhard, Krackhardt, Angela, Hecker, Judith S, Bassermann, Florian

“…Chimeric antigen receptor (CAR) T cells exhibit high response rates in B cell malignancies, but most patients eventually relapse. A key mechanism of treatment…”
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Spatial profiling of gene editing by in situ sequencing in mice and macaques by Janjuha, Sharan, Haenggi, Tatjana, Chamberlain, Thomas C, Rothgangl, Tanja, Kissling, Lucas, Wilhelm, Maria, Mathis, Nicolas, Boeck, Desiree, Marquart, Kim, Ioannidi, Eleonora, Moon, Woohyun J, Muramatsu, Hiromi, Vadovics, Máté, Pardi, Norbert, Semple, Sean C, Tam, Ying K, Schwank, Gerald

“…Base and prime editing technologies precisely install defined nucleotide edits in both dividing and non-dividing cells, offering potential for correcting…”
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Characterization of two novel intronic OPA1 mutations resulting in aberrant pre-mRNA splicing by Bolognini, Ramona, Gerth-Kahlert, Christina, Abegg, Mathias, Bartholdi, Deborah, Mathis, Nicolas, Sturm, Veit, Gallati, Sabina, Schaller, André

“…Background We report two novel splice region mutations in OPA1 in two unrelated families presenting with autosomal-dominant optic atrophy type 1 (ADOA1) (ADOA…”
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Osteoblast interaction with DLC-coated Si substrates by Chai, Feng, Mathis, Nicolas, Blanchemain, Nicolas, Meunier, Cathy, Hildebrand, Hartmut F.

“…Diamond-like carbon (DLC) coating is a convenient means of modifying material surfaces that are sensitive to wear, such as titanium and silica substrates. This…”
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Simultaneous effects of nutritional and environmental factors on growth and flesh quality of Perca fluviatilis using a fractional factorial design study by Gardeur, Jean-Noel, Mathis, Nicolas, Kobilinsky, Andre, Brun-Bellut, Jean

“…Fractional factorial design is a practical approach for studying multiple factors, with a minimum of experimental units. The objective of this work was to…”
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Ultrastructural features of hepatocytes in cultured Eurasian perch (Perca fluviatilis L.) as affected by nutritional and husbandry conditions by Blanchard, Gersande, Gardeur, Jean N., Mathis, Nicolas, Brun-Bellut, Jean, Kestemont, Patrick

“…A wide range of factors can be attributed to the syndrome of fatty liver observed in some cultured fish species. The objective of the study was therefore to…”
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Predicting prime editing efficiency across diverse edit types and chromatin contexts with machine learning by Mathis, Nicolas, Allam, Ahmed, Tálas, András, Benvenuto, Elena, Schep, Ruben, Damodharan, Tanav, Balázs, Zsolt, Janjuha, Sharan, Schmidheini, Lukas, Böck, Desirée, van Steensel, Bas, Krauthammer, Michael, Schwank, Gerald

“…Prime editing is a powerful genome editing technology, but its efficiency varies depending on the pegRNA design and target locus. Existing computational models…”
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