Search Results - "Lopes, Massimo"

The plasticity of DNA replication forks in response to clinically relevant genotoxic stress by Berti, Matteo, Cortez, David, Lopes, Massimo

“…Complete and accurate DNA replication requires the progression of replication forks through DNA damage, actively transcribed regions, structured DNA and…”
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Rad51-mediated replication fork reversal is a global response to genotoxic treatments in human cells by Zellweger, Ralph, Dalcher, Damian, Mutreja, Karun, Berti, Matteo, Schmid, Jonas A, Herrador, Raquel, Vindigni, Alessandro, Lopes, Massimo

“…Replication fork reversal protects forks from breakage after poisoning of Topoisomerase 1. We here investigated fork progression and chromosomal breakage in…”
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Replication fork reversal triggers fork degradation in BRCA2-defective cells by Mijic, Sofija, Zellweger, Ralph, Chappidi, Nagaraja, Berti, Matteo, Jacobs, Kurt, Mutreja, Karun, Ursich, Sebastian, Ray Chaudhuri, Arnab, Nussenzweig, Andre, Janscak, Pavel, Lopes, Massimo

“…Besides its role in homologous recombination, the tumor suppressor BRCA2 protects stalled replication forks from nucleolytic degradation. Defective fork…”
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Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis by Hashimoto, Yoshitami, Ray Chaudhuri, Arnab, Lopes, Massimo, Costanzo, Vincenzo

“…Rad51 plays a central role in homologous recombination pathways. Now work with Xenopus laevis egg extracts shows that Rad51 prevents formation of DNA gaps at…”
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Sequential role of RAD51 paralog complexes in replication fork remodeling and restart by Berti, Matteo, Teloni, Federico, Mijic, Sofija, Ursich, Sebastian, Fuchs, Jevgenij, Palumbieri, Maria Dilia, Krietsch, Jana, Schmid, Jonas A., Garcin, Edwige B., Gon, Stéphanie, Modesti, Mauro, Altmeyer, Matthias, Lopes, Massimo

“…Homologous recombination (HR) factors were recently implicated in DNA replication fork remodeling and protection. While maintaining genome stability,…”
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Fork Cleavage-Religation Cycle and Active Transcription Mediate Replication Restart after Fork Stalling at Co-transcriptional R-Loops by Chappidi, Nagaraja, Nascakova, Zuzana, Boleslavska, Barbora, Zellweger, Ralph, Isik, Esin, Andrs, Martin, Menon, Shruti, Dobrovolna, Jana, Balbo Pogliano, Chiara, Matos, Joao, Porro, Antonio, Lopes, Massimo, Janscak, Pavel

“…Formation of co-transcriptional R-loops underlies replication fork stalling upon head-on transcription-replication encounters. Here, we demonstrate that…”
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HLTF Promotes Fork Reversal, Limiting Replication Stress Resistance and Preventing Multiple Mechanisms of Unrestrained DNA Synthesis by Bai, Gongshi, Kermi, Chames, Stoy, Henriette, Schiltz, Carl J, Bacal, Julien, Zaino, Angela M, Hadden, M Kyle, Eichman, Brandt F, Lopes, Massimo, Cimprich, Karlene A

“…DNA replication stress can stall replication forks, leading to genome instability. DNA damage tolerance pathways assist fork progression, promoting replication…”
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DNA2 drives processing and restart of reversed replication forks in human cells by Thangavel, Saravanabhavan, Berti, Matteo, Levikova, Maryna, Pinto, Cosimo, Gomathinayagam, Shivasankari, Vujanovic, Marko, Zellweger, Ralph, Moore, Hayley, Lee, Eu Han, Hendrickson, Eric A, Cejka, Petr, Stewart, Sheila, Lopes, Massimo, Vindigni, Alessandro

“…Accurate processing of stalled or damaged DNA replication forks is paramount to genomic integrity and recent work points to replication fork reversal and…”
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Visualization of recombination-mediated damage bypass by template switching by Giannattasio, Michele, Zwicky, Katharina, Follonier, Cindy, Foiani, Marco, Lopes, Massimo, Branzei, Dana

“…A combination of two-dimensional gel electrophoresis and EM is used to isolate and characterize multiple template-switching intermediates generated in budding…”
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Replication Fork Slowing and Reversal upon DNA Damage Require PCNA Polyubiquitination and ZRANB3 DNA Translocase Activity by Vujanovic, Marko, Krietsch, Jana, Raso, Maria Chiara, Terraneo, Nastassja, Zellweger, Ralph, Schmid, Jonas A, Taglialatela, Angelo, Huang, Jen-Wei, Holland, Cory L, Zwicky, Katharina, Herrador, Raquel, Jacobs, Heinz, Cortez, David, Ciccia, Alberto, Penengo, Lorenza, Lopes, Massimo

“…DNA damage tolerance during eukaryotic replication is orchestrated by PCNA ubiquitination. While monoubiquitination activates mutagenic translesion synthesis,…”
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Excessive reactive oxygen species induce transcription-dependent replication stress by Andrs, Martin, Stoy, Henriette, Boleslavska, Barbora, Chappidi, Nagaraja, Kanagaraj, Radhakrishnan, Nascakova, Zuzana, Menon, Shruti, Rao, Satyajeet, Oravetzova, Anna, Dobrovolna, Jana, Surendranath, Kalpana, Lopes, Massimo, Janscak, Pavel

“…Elevated levels of reactive oxygen species (ROS) reduce replication fork velocity by causing dissociation of the TIMELESS-TIPIN complex from the replisome…”
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Topoisomerase I poisoning results in PARP-mediated replication fork reversal by Ray Chaudhuri, Arnab, Hashimoto, Yoshitami, Herrador, Raquel, Neelsen, Kai J, Fachinetti, Daniele, Bermejo, Rodrigo, Cocito, Andrea, Costanzo, Vincenzo, Lopes, Massimo

“…Topoisomerase 1 (Top1) inhibition is believed to mediate cellular toxicity by trapping Top1 on nicked DNA, leading to double-strand break formation during…”
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Selective Loss of PARG Restores PARylation and Counteracts PARP Inhibitor-Mediated Synthetic Lethality by Gogola, Ewa, Duarte, Alexandra A, de Ruiter, Julian R, Wiegant, Wouter W, Schmid, Jonas A, de Bruijn, Roebi, James, Dominic I, Guerrero Llobet, Sergi, Vis, Daniel J, Annunziato, Stefano, van den Broek, Bram, Barazas, Marco, Kersbergen, Ariena, van de Ven, Marieke, Tarsounas, Madalena, Ogilvie, Donald J, van Vugt, Marcel, Wessels, Lodewyk F A, Bartkova, Jirina, Gromova, Irina, Andújar-Sánchez, Miguel, Bartek, Jiri, Lopes, Massimo, van Attikum, Haico, Borst, Piet, Jonkers, Jos, Rottenberg, Sven

“…Inhibitors of poly(ADP-ribose) (PAR) polymerase (PARPi) have recently entered the clinic for the treatment of homologous recombination (HR)-deficient cancers…”
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A short G1 phase imposes constitutive replication stress and fork remodelling in mouse embryonic stem cells by Ahuja, Akshay K., Jodkowska, Karolina, Teloni, Federico, Bizard, Anna H., Zellweger, Ralph, Herrador, Raquel, Ortega, Sagrario, Hickson, Ian D., Altmeyer, Matthias, Mendez, Juan, Lopes, Massimo

“…Embryonic stem cells (ESCs) represent a transient biological state, where pluripotency is coupled with fast proliferation. ESCs display a constitutively active…”
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Oncogenes induce genotoxic stress by mitotic processing of unusual replication intermediates by Neelsen, Kai J, Zanini, Isabella M Y, Herrador, Raquel, Lopes, Massimo

“…Oncogene-induced DNA replication stress activates the DNA damage response (DDR), a crucial anticancer barrier. DDR inactivation in these conditions promotes…”
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ATR-Mediated Global Fork Slowing and Reversal Assist Fork Traverse and Prevent Chromosomal Breakage at DNA Interstrand Cross-Links by Mutreja, Karun, Krietsch, Jana, Hess, Jeannine, Ursich, Sebastian, Berti, Matteo, Roessler, Fabienne K., Zellweger, Ralph, Patra, Malay, Gasser, Gilles, Lopes, Massimo

“…Interstrand cross-links (ICLs) are toxic DNA lesions interfering with DNA metabolism that are induced by widely used anticancer drugs. They have long been…”
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Human RECQ1 promotes restart of replication forks reversed by DNA topoisomerase I inhibition by Berti, Matteo, Ray Chaudhuri, Arnab, Thangavel, Saravanabhavan, Gomathinayagam, Shivasankari, Kenig, Sasa, Vujanovic, Marko, Odreman, Federico, Glatter, Timo, Graziano, Simona, Mendoza-Maldonado, Ramiro, Marino, Francesca, Lucic, Bojana, Biasin, Valentina, Gstaiger, Matthias, Aebersold, Ruedi, Sidorova, Julia M, Monnat, Raymond J, Lopes, Massimo, Vindigni, Alessandro

“…The cytotoxic effects of topoisomerase I inhibitors such as camptothecin can be modulated by replication fork reversal, in a process that requires…”
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Dynamic Architecture of Eukaryotic DNA Replication Forks In Vivo, Visualized by Electron Microscopy by Zellweger, Ralph, Lopes, Massimo

“…The DNA replication process can be heavily perturbed by several different conditions of genotoxic stress, particularly relevant for cancer onset and therapy…”
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Human RIF1-Protein Phosphatase 1 Prevents Degradation and Breakage of Nascent DNA on Replication Stalling by Garzón, Javier, Ursich, Sebastian, Lopes, Massimo, Hiraga, Shin-ichiro, Donaldson, Anne D.

“…RIF1 is a multifunctional protein implicated in controlling DNA replication and repair. Here, we show that human RIF1 protects nascent DNA from…”
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Nuclear actin polymerization rapidly mediates replication fork remodeling upon stress by limiting PrimPol activity by Palumbieri, Maria Dilia, Merigliano, Chiara, González-Acosta, Daniel, Kuster, Danina, Krietsch, Jana, Stoy, Henriette, von Känel, Thomas, Ulferts, Svenja, Welter, Bettina, Frey, Joël, Doerdelmann, Cyril, Sanchi, Andrea, Grosse, Robert, Chiolo, Irene, Lopes, Massimo

“…Cells rapidly respond to replication stress actively slowing fork progression and inducing fork reversal. How replication fork plasticity is achieved in the…”
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