Výsledky vyhľadávania - "Journal of virological methods"

Comparison of the SARS-CoV-2 Rapid antigen test to the real star Sars-CoV-2 RT PCR kit Autor Krüttgen, Alexander, Cornelissen, Christian G., Dreher, Michael, Hornef, Mathias W., Imöhl, Matthias, Kleines, Michael

“…•The sensitivity and specificity of the new Roche SARS-CoV-2 Rapid Antigen Test was evaluated.•We found a specificity of 96 %.•The sensitivity with samples…”
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Performance evaluation of the Roche Elecsys Anti-SARS-CoV-2 S immunoassay Autor Riester, Elena, Findeisen, Peter, Hegel, J. Kolja, Kabesch, Michael, Ambrosch, Andreas, Rank, Christopher M., Pessl, Florina, Laengin, Tina, Niederhauser, Christoph

“…•The Elecsys® Anti-SARS-CoV-2 S assay detects antibodies to the SARS-CoV-2 S protein.•This assay demonstrated an overall specificity of 99.95% (7876/7880…”
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The screening value of RT-LAMP and RT-PCR in the diagnosis of COVID-19: systematic review and meta-analysis Autor Pu, Ruiyang, Liu, Sha, Ren, Xiaoyu, Shi, Dian, Ba, Yupei, Huo, Yanbei, Zhang, Wenling, Ma, Lingling, Liu, Yanyan, Yang, Yan, Cheng, Ning

“…•RT-PCR and RT-LAMP are widely used; however false negative rate (FNR) are 6% ∼ 12 %.•Sensitivity and FNR of index test were significantly affected by the…”
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Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay Autor Mariën, Joachim, Ceulemans, Ann, Michiels, Johan, Heyndrickx, Leo, Kerkhof, Karen, Foque, Nikki, Widdowson, Marc-Alain, Mortgat, Laure, Duysburgh, Els, Desombere, Isabelle, Jansens, Hilde, Van Esbroeck, Marjan, Ariën, Kevin K.

“…•We evaluated a bead-based Luminex assay for the detection of SARS-CoV-2 antibodies.•A combination of the nucleocapsid protein and the receptor-binding domain…”
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Inhibition of herpes simplex virus type 1 by copper oxide nanoparticles Autor Tavakoli, Ahmad, Hashemzadeh, Mohammad Sadegh

“…•Copper oxide (CuO-NPs) nanoparticles significantly inhibit HSV-1 infection.•The inhibition occurs when CuO-NPs are added after virus adsorption to the…”
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Assessing sensitivity and reproducibility of RT-ddPCR and RT-qPCR for the quantification of SARS-CoV-2 in wastewater Autor Ciesielski, Mark, Blackwood, Denene, Clerkin, Thomas, Gonzalez, Raul, Thompson, Hannah, Larson, Allison, Noble, Rachel

“…[Display omitted] •Wastewater surveillance methods captured the emergence of SARS-CoV-2 presence in a community.•In a direct comparison of workflows, RT-ddPCR…”
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Comparison of potency assays to assess SARS-CoV-2 neutralizing antibody capacity in COVID-19 convalescent plasma Autor von Rhein, Christine, Scholz, Tatjana, Henss, Lisa, Kronstein-Wiedemann, Romy, Schwarz, Tatjana, Rodionov, Roman N., Corman, Victor M., Tonn, Torsten, Schnierle, Barbara S.

“…•Transfusion of sera of convalescent patients is currently in clinical evaluations to treat COVID-19 patients.•Neutralizing antibody levels vary among…”
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Development of a real-time loop-mediated isothermal amplification (LAMP) assay and visual LAMP assay for detection of African swine fever virus (ASFV) Autor Wang, Deguo, Yu, Jianghan, Wang, Yongzhen, Zhang, Meng, Li, Peng, Liu, Meng, Liu, Yanhong

“…•LAMP assays were developed for detection of African swine fever (ASF).•The LAMP assays were comparable to the well-established real-time PCR assay.•The LAMP…”
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SARS-CoV-2: Comparative analysis of different RNA extraction methods Autor Ambrosi, Cecilia, Prezioso, Carla, Checconi, Paola, Scribano, Daniela, Sarshar, Meysam, Capannari, Maurizio, Tomino, Carlo, Fini, Massimo, Garaci, Enrico, Palamara, Anna Teresa, De Chiara, Giovanna, Limongi, Dolores

“…[Display omitted] •Alternative suitable methods to extract SARS-CoV-2 RNA should be taken into account to cope with the ongoing pandemic.•Extraction…”
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Time series analysis and predicting COVID-19 affected patients by ARIMA model using machine learning Autor Chyon, Fuad Ahmed, Suman, Md. Nazmul Hasan, Fahim, Md. Rafiul Islam, Ahmmed, Md. Sazol

“…•The purpose of this study is to find the future forecast scenario of the confirmed case-patients.•We are using the ARIMA time series model for our prediction…”
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Cytotoxicity and antiviral activity of electrochemical – synthesized silver nanoparticles against poliovirus Autor Huy, Tran Quang, Hien Thanh, Nguyen Thi, Thuy, Nguyen Thanh, Chung, Pham Van, Hung, Pham Ngoc, Le, Anh-Tuan, Hong Hanh, Nguyen Thi

“…•Silver nanoparticles (AgNPs) have been eco-friendly synthesized by the electrochemical method.•Electrochemical – synthesized AgNPs are non-toxic to cell…”
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Combination of serological total antibody and RT-PCR test for detection of SARS-COV-2 infections Autor Wang, Pei

“…•Combination of Wantai Total Antibody assay and RT-PCR Test for detection of SARS-COV-2 Infections.•Wantai total antibody test by Chemiluminescence…”
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Improving human coronavirus OC43 (HCoV-OC43) research comparability in studies using HCoV-OC43 as a surrogate for SARS-CoV-2 Autor Schirtzinger, Erin E., Kim, Yunjeong, Davis, A. Sally

“…•HCoV-OC43 grows to a higher titer, sooner in human lung fibroblasts (MRC-5) than in human ileocecal adenocarcinoma cells (HRT-18).•Optimization of an…”
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Evaluation of the QuantiFERON SARS-CoV-2 interferon-ɣ release assay in mRNA-1273 vaccinated health care workers Autor Krüttgen, Alexander, Klingel, Hanna, Haase, Gerhard, Haefner, Helga, Imöhl, Matthias, Kleines, Michael

“…•44 % of vaccinees had INF-ɣ levels above 015 IU/mL in response to stimulation.•100 % of vaccinees showed INF-ɣ levels above 0015 IU/mL in response to…”
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Analysis of preferred codon usage in the coronavirus N genes and their implications for genome evolution and vaccine design Autor Sheikh, Abdullah, Al-Taher, Abdulla, Al-Nazawi, Mohammed, Al-Mubarak, Abdullah I., Kandeel, Mahmoud

“…•The nucleotide variations among the N genes of 13 different coronaviruses (CoVs) were interpreted.•Overall, 18 amino acids observed with varying preferred…”
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A combined approach of MALDI-TOF mass spectrometry and multivariate analysis as a potential tool for the detection of SARS-CoV-2 virus in nasopharyngeal swabs Autor Rocca, María Florencia, Zintgraff, Jonathan Cristian, Dattero, María Elena, Santos, Leonardo Silva, Ledesma, Martín, Vay, Carlos, Prieto, Mónica, Benedetti, Estefanía, Avaro, Martín, Russo, Mara, Nachtigall, Fabiane Manke, Baumeister, Elsa

“…•This study with MALDI-TOF comprises, as far as we know, the first report describing the performance of this technology with COVID-19 diagnosis.•This work…”
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Development of fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) using quenching probes for the detection of the Middle East respiratory syndrome coronavirus Autor Shirato, Kazuya, Semba, Shohei, El-Kafrawy, Sherif A., Hassan, Ahmed M., Tolah, Ahmed M., Takayama, Ikuyo, Kageyama, Tsutomu, Notomi, Tsugunori, Kamitani, Wataru, Matsuyama, Shutoku, Azhar, Esam Ibraheem

“…•Fluorescent RT-LAMP assays using quenching probes for MERS-CoV were developed.•Quenching probe (QProbe) can solve the problem in turbidity monitoring…”
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Isothermal recombinase polymerase amplification-lateral flow detection of SARS-CoV-2, the etiological agent of COVID-19 Autor Shelite, Thomas R., Uscanga-Palomeque, Ashanti C., Castellanos-Gonzalez, Alejandro, Melby, Peter C., Travi, Bruno L.

“…•Isothermal, rapid amplification and lateral flow qualitative detection of SARS-CoV-2.•Two-step, point-of-care diagnostic development for…”
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The effect of heat-treatment on SARS-CoV-2 viability and detection Autor Burton, Jane, Love, Hannah, Richards, Kevin, Burton, Christopher, Summers, Sian, Pitman, James, Easterbrook, Linda, Davies, Katherine, Spencer, Peter, Killip, Marian, Cane, Patricia, Bruce, Christine, Roberts, Allen D.G.

“…•SARS-CoV-2 is not fully inactivated by heating to 56 °C or 60 °C for up to 60 min.•Complete inactivation occurred at 80 °C for 90 min or 95 °C for 1 or 5…”
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Genomic and evolutionary comparison between SARS-CoV-2 and other human coronaviruses Autor Chen, Zigui, Boon, Siaw S., Wang, Maggie H., Chan, Renee W.Y., Chan, Paul K.S.

“…Three highly pathogenic human coronaviruses can cause severe acute respiratory syndrome (SARS-CoV, SARS-CoV-2 and MERS-CoV). Although phylogenetic analyses…”
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