Suchergebnisse - "Dedecker, Peter"

Optimizing the fluorescent protein toolbox and its use von Duwé, Sam, Dedecker, Peter

“… [Display omitted] •Fluorescent proteins continue to be essential tools in life sciences.•Phototransformable FPs and biosensors facilitate advanced imaging …”
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Orthogonal fluorescent chemogenetic reporters for multicolor imaging von Tebo, Alison G., Moeyaert, Benjamien, Thauvin, Marion, Carlon-Andres, Irene, Böken, Dorothea, Volovitch, Michel, Padilla-Parra, Sergi, Dedecker, Peter, Vriz, Sophie, Gautier, Arnaud

“… Spectrally separated fluorophores allow the observation of multiple targets simultaneously inside living cells, leading to a deeper understanding of the …”
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Widely accessible method for superresolution fluorescence imaging of living systems von Dedecker, Peter, Mo, Gary C H, Dertinger, Thomas, Zhang, Jin

“… Superresolution fluorescence microscopy overcomes the diffraction resolution barrier and allows the molecular intricacies of life to be revealed with greatly …”
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Simultaneous readout of multiple FRET pairs using photochromism von Roebroek, Thijs, Vandenberg, Wim, Sipieter, François, Hugelier, Siewert, Stove, Christophe, Zhang, Jin, Dedecker, Peter

“… Förster resonant energy transfer (FRET) is a powerful mechanism to probe associations in situ. Simultaneously performing more than one FRET measurement can be …”
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Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution von Mo, Gary C H, Ross, Brian, Hertel, Fabian, Manna, Premashis, Yang, Xinxing, Greenwald, Eric, Booth, Chris, Plummer, Ashlee M, Tenner, Brian, Chen, Zan, Wang, Yuxiao, Kennedy, Eileen J, Cole, Philip A, Fleming, Karen G, Palmer, Amy, Jimenez, Ralph, Xiao, Jie, Dedecker, Peter, Zhang, Jin

“… New fluorescent biosensors enable the first super-resolution imaging of enzyme activity in live cells via fluorescence fluctuation increase by contact (FLINC) …”
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Localizer: fast, accurate, open-source, and modular software package for superresolution microscopy von Dedecker, Peter, Duwé, Sam, Neely, Robert K, Zhang, Jin

“… We present Localizer, a freely available and open source software package that implements the computational data processing inherent to several types of …”
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Actuation enhances patterning in human neural tube organoids von Abdel Fattah, Abdel Rahman, Daza, Brian, Rustandi, Gregorius, Berrocal-Rubio, Miguel Ángel, Gorissen, Benjamin, Poovathingal, Suresh, Davie, Kristofer, Barrasa-Fano, Jorge, Cóndor, Mar, Cao, Xuanye, Rosenzweig, Derek Hadar, Lei, Yunping, Finnell, Richard, Verfaillie, Catherine, Sampaolesi, Maurilio, Dedecker, Peter, Van Oosterwyck, Hans, Aerts, Stein, Ranga, Adrian

“… Tissues achieve their complex spatial organization through an interplay between gene regulatory networks, cell-cell communication, and physical interactions …”
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Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification von Bierbuesse, Franziska, Bourges, Anaïs C., Gielen, Vincent, Mönkemöller, Viola, Vandenberg, Wim, Shen, Yi, Hofkens, Johan, Vanden Berghe, Pieter, Campbell, Robert E., Moeyaert, Benjamien, Dedecker, Peter

“… Genetically-encoded biosensors based on a single fluorescent protein are widely used to visualize analyte levels or enzymatic activities in cells, though …”
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Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions von Deschout, Hendrik, Lukes, Tomas, Sharipov, Azat, Szlag, Daniel, Feletti, Lely, Vandenberg, Wim, Dedecker, Peter, Hofkens, Johan, Leutenegger, Marcel, Lasser, Theo, Radenovic, Aleksandra

“… Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we …”
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SOFI Simulation Tool: A Software Package for Simulating and Testing Super-Resolution Optical Fluctuation Imaging von Girsault, Arik, Lukes, Tomas, Sharipov, Azat, Geissbuehler, Stefan, Leutenegger, Marcel, Vandenberg, Wim, Dedecker, Peter, Hofkens, Johan, Lasser, Theo

“… Super-resolution optical fluctuation imaging (SOFI) allows one to perform sub-diffraction fluorescence microscopy of living cells. By analyzing the acquired …”
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Mechanistic Investigations of Green mEos4b Reveal a Dynamic Long-Lived Dark State von De Zitter, Elke, Ridard, Jacqueline, Thédié, Daniel, Adam, Virgile, Lévy, Bernard, Byrdin, Martin, Gotthard, Guillaume, Van Meervelt, Luc, Dedecker, Peter, Demachy, Isabelle, Bourgeois, Dominique

“… Green-to-red photoconvertible fluorescent proteins (PCFPs) are key players in advanced microscopy schemes such as photoactivated localization microscopy …”
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Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM von De Zitter, Elke, Thédié, Daniel, Mönkemöller, Viola, Hugelier, Siewert, Beaudouin, Joël, Adam, Virgile, Byrdin, Martin, Van Meervelt, Luc, Dedecker, Peter, Bourgeois, Dominique

“… Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy …”
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The Persistence-Inducing Toxin HokB Forms Dynamic Pores That Cause ATP Leakage von Wilmaerts, Dorien, Bayoumi, Mariam, Dewachter, Liselot, Knapen, Wouter, Mika, Jacek T., Hofkens, Johan, Dedecker, Peter, Maglia, Giovanni, Verstraeten, Natalie, Michiels, Jan

“… Bacterial populations harbor a small fraction of cells that display transient multidrug tolerance. These so-called persister cells are extremely difficult to …”
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Structure–Function Dataset Reveals Environment Effects within a Fluorescent Protein Model System von De Zitter, Elke, Hugelier, Siewert, Duwé, Sam, Vandenberg, Wim, Tebo, Alison G., Van Meervelt, Luc, Dedecker, Peter

“… Anisotropic environments can drastically alter the spectroscopy and photochemistry of molecules, leading to complex structure‐function relationships. We …”
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Expression-Enhanced Fluorescent Proteins Based on Enhanced Green Fluorescent Protein for Super-resolution Microscopy von Duwé, Sam, De Zitter, Elke, Gielen, Vincent, Moeyaert, Benjamien, Vandenberg, Wim, Grotjohann, Tim, Clays, Koen, Jakobs, Stefan, Van Meervelt, Luc, Dedecker, Peter

“… “Smart fluorophores”, such as reversibly switchable fluorescent proteins, are crucial for advanced fluorescence imaging. However, only a limited number of such …”
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High-Resolution Single-Molecule Fluorescence Imaging of Zeolite Aggregates within Real-Life Fluid Catalytic Cracking Particles von Ristanović, Zoran, Kerssens, Marleen M., Kubarev, Alexey V., Hendriks, Frank C., Dedecker, Peter, Hofkens, Johan, Roeffaers, Maarten B. J., Weckhuysen, Bert M.

“… Fluid catalytic cracking (FCC) is a major process in oil refineries to produce gasoline and base chemicals from crude oil fractions. The spatial distribution …”
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Green-to-Red Photoconvertible Dronpa Mutant for Multimodal Super-resolution Fluorescence Microscopy von Moeyaert, Benjamien, Nguyen Bich, Ngan, De Zitter, Elke, Rocha, Susana, Clays, Koen, Mizuno, Hideaki, van Meervelt, Luc, Hofkens, Johan, Dedecker, Peter

“… Advanced imaging techniques crucially depend on the labels used. In this work, we present the structure-guided design of a fluorescent protein that displays …”
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Author Correction: Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification von Bierbuesse, Franziska, Bourges, Anaïs C., Gielen, Vincent, Mönkemöller, Viola, Vandenberg, Wim, Shen, Yi, Hofkens, Johan, Vanden Berghe, Pieter, Campbell, Robert E., Moeyaert, Benjamien, Dedecker, Peter

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Reduced Fluorescent Protein Switching Fatigue by Binding-Induced Emissive State Stabilization von Roebroek, Thijs, Duwé, Sam, Vandenberg, Wim, Dedecker, Peter

“… Reversibly switchable fluorescent proteins (RSFPs) enable advanced fluorescence imaging, though the performance of this imaging crucially depends on the …”
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A comprehensive dataset of image sequences covering 20 fluorescent protein labels and 12 imaging conditions for use in super-resolution imaging von Moeyaert, Benjamien, Dedecker, Peter

“… Super-resolution fluorescence microscopy techniques allow imaging fluorescently labelled structures with a resolution that surpasses the diffraction limit of …”
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