Suchergebnisse - "Cimprich, Karlene A."

The essential kinase ATR: ensuring faithful duplication of a challenging genome von Saldivar, Joshua C., Cortez, David, Cimprich, Karlene A.

“… Key Points Ataxia telangiectasia and Rad3-related (ATR) is an essential kinase that is active in S phase, senses stressed replication forks and orchestrates a …”
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Transcription-Replication Conflict Orientation Modulates R-Loop Levels and Activates Distinct DNA Damage Responses von Hamperl, Stephan, Bocek, Michael J, Saldivar, Joshua C, Swigut, Tomek, Cimprich, Karlene A

“… Conflicts between transcription and replication are a potent source of DNA damage. Co-transcriptional R-loops could aggravate such conflicts by creating an …”
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The contribution of co-transcriptional RNA:DNA hybrid structures to DNA damage and genome instability von Hamperl, Stephan, Cimprich, Karlene A.

“… Accurate DNA replication and DNA repair are crucial for the maintenance of genome stability, and it is generally accepted that failure of these processes is a …”
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Transcription-coupled nucleotide excision repair factors promote R-loop-induced genome instability von Sollier, Julie, Stork, Caroline Townsend, García-Rubio, María L, Paulsen, Renee D, Aguilera, Andrés, Cimprich, Karlene A

“… R-loops, consisting of an RNA-DNA hybrid and displaced single-stranded DNA, are physiological structures that regulate various cellular processes occurring on …”
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qDRIP: a method to quantitatively assess RNA–DNA hybrid formation genome-wide von Crossley, Magdalena P, Bocek, Michael J, Hamperl, Stephan, Swigut, Tomek, Cimprich, Karlene A

“… Abstract R-loops are dynamic, co-transcriptional nucleic acid structures that facilitate physiological processes but can also cause DNA damage in certain …”
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Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells von Hess, Gaelen T, Frésard, Laure, Han, Kyuho, Lee, Cameron H, Li, Amy, Cimprich, Karlene A, Montgomery, Stephen B, Bassik, Michael C

“… Recruiting a hyperactive cytidine deaminase via the guide RNA to dCas9 allows for the introduction of diverse point mutations at the CRISPR target locus to …”
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HLTF Promotes Fork Reversal, Limiting Replication Stress Resistance and Preventing Multiple Mechanisms of Unrestrained DNA Synthesis von Bai, Gongshi, Kermi, Chames, Stoy, Henriette, Schiltz, Carl J, Bacal, Julien, Zaino, Angela M, Hadden, M Kyle, Eichman, Brandt F, Lopes, Massimo, Cimprich, Karlene A

“… DNA replication stress can stall replication forks, leading to genome instability. DNA damage tolerance pathways assist fork progression, promoting replication …”
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A role for the MRN complex in ATR activation via TOPBP1 recruitment von Duursma, Anja M, Driscoll, Robert, Elias, Josh E, Cimprich, Karlene A

“… The MRN (MRE11-RAD50-NBS1) complex has been implicated in many aspects of the DNA damage response. It has key roles in sensing and processing DNA double-strand …”
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HLTF's Ancient HIRAN Domain Binds 3' DNA Ends to Drive Replication Fork Reversal von Kile, Andrew C, Chavez, Diana A, Bacal, Julien, Eldirany, Sherif, Korzhnev, Dmitry M, Bezsonova, Irina, Eichman, Brandt F, Cimprich, Karlene A

“… Stalled replication forks are a critical problem for the cell because they can lead to complex genome rearrangements that underlie cell death and disease …”
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A two-dimensional ERK-AKT signaling code for an NGF-triggered cell-fate decision von Chen, Jia-Yun, Lin, Jia-Ren, Cimprich, Karlene A, Meyer, Tobias

“… Growth factors activate Ras, PI3K, and other signaling pathways. It is not well understood how these signals are translated by individual cells into a decision …”
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SHPRH and HLTF act in a damage-specific manner to coordinate different forms of postreplication repair and prevent mutagenesis von Lin, Jia-Ren, Zeman, Michelle K, Chen, Jia-Yun, Yee, Muh-Ching, Cimprich, Karlene A

“… Postreplication repair (PRR) pathways play important roles in restarting stalled replication forks and regulating mutagenesis. In yeast, Rad5-mediated damage …”
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Catalytically inactive, purified RNase H1: A specific and sensitive probe for RNA-DNA hybrid imaging von Crossley, Magdalena P, Brickner, Joshua R, Song, Chenlin, Zar, Su Mon Thin, Maw, Su S, Chédin, Frédéric, Tsai, Miaw-Sheue, Cimprich, Karlene A

“… R-loops are three-stranded nucleic acid structures with both physiological and pathological roles in cells. R-loop imaging generally relies on detection of the …”
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Co-transcriptional R-loops are the main cause of estrogen-induced DNA damage von Stork, Caroline Townsend, Bocek, Michael, Crossley, Madzia P, Sollier, Julie, Sanz, Lionel A, Chédin, Frédéric, Swigut, Tomek, Cimprich, Karlene A

“… The hormone estrogen (E2) binds the estrogen receptor to promote transcription of E2-responsive genes in the breast and other tissues. E2 also has links to …”
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DNA damage tolerance: when it's OK to make mistakes von Chang, Debbie J, Cimprich, Karlene A

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ATR phosphorylates SMARCAL1 to prevent replication fork collapse von Couch, Frank B, Bansbach, Carol E, Driscoll, Robert, Luzwick, Jessica W, Glick, Gloria G, Bétous, Rémy, Carroll, Clinton M, Jung, Sung Yun, Qin, Jun, Cimprich, Karlene A, Cortez, David

“… The DNA damage response kinase ataxia telangiectasia and Rad3-related (ATR) coordinates much of the cellular response to replication stress. The exact …”
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PPARγ Interaction with UBR5/ATMIN Promotes DNA Repair to Maintain Endothelial Homeostasis von Li, Caiyun G., Mahon, Cathal, Sweeney, Nathaly M., Verschueren, Erik, Kantamani, Vivek, Li, Dan, Hennigs, Jan K., Marciano, David P., Diebold, Isabel, Abu-Halawa, Ossama, Elliott, Matthew, Sa, Silin, Guo, Feng, Wang, Lingli, Cao, Aiqin, Guignabert, Christophe, Sollier, Julie, Nickel, Nils P., Kaschwich, Mark, Cimprich, Karlene A., Rabinovitch, Marlene

“… Using proteomic approaches, we uncovered a DNA damage response (DDR) function for peroxisome proliferator activated receptor γ (PPARγ) through its interaction …”
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NEK8 links the ATR-regulated replication stress response and S phase CDK activity to renal ciliopathies von Choi, Hyo Jei Claudia, Lin, Jia-Ren, Vannier, Jean-Baptiste, Slaats, Gisela G, Kile, Andrew C, Paulsen, Renee D, Manning, Danielle K, Beier, David R, Giles, Rachel H, Boulton, Simon J, Cimprich, Karlene A

“… Renal ciliopathies are a leading cause of kidney failure, but their exact etiology is poorly understood. NEK8/NPHP9 is a ciliary kinase associated with two …”
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Functional uncoupling of MCM helicase and DNA polymerase activities activates the ATR-dependent checkpoint von Byun, Tony S, Pacek, Marcin, Yee, Muh-ching, Walter, Johannes C, Cimprich, Karlene A

“… The ATR-dependent DNA damage response pathway can respond to a diverse group of lesions as well as inhibitors of DNA replication. Using the Xenopus egg extract …”
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The structural determinants of checkpoint activation von MacDougall, Christina A, Byun, Tony S, Van, Christopher, Yee, Muh-ching, Cimprich, Karlene A

“… Here, we demonstrate that primed, single-stranded DNA (ssDNA) is sufficient for activation of the ATR-dependent checkpoint pathway in Xenopus egg extracts …”
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R-Loops as Cellular Regulators and Genomic Threats von Crossley, Madzia P, Bocek, Michael, Cimprich, Karlene A

“… During transcription, the nascent RNA strand can base pair with its template DNA, displacing the non-template strand as ssDNA and forming a structure called an …”
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