Search Results - "Chromatography, Gel"

Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium by Dong, Liang, Zieren, Richard C., Horie, Kengo, Kim, Chi‐Ju, Mallick, Emily, Jing, Yuezhou, Feng, Mingxiao, Kuczler, Morgan D., Green, Jordan, Amend, Sarah R., Witwer, Kenneth W., de Reijke, Theo M., Cho, Yoon‐Kyoung, Pienta, Kenneth J., Xue, Wei

“…One of the challenges that restricts the evolving extracellular vesicle (EV) research field is the lack of a consensus method for EV separation. This may also…”
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Valosin-containing protein (VCP)-Adaptor Interactions are Exceptionally Dynamic and Subject to Differential Modulation by a VCP Inhibitor by Xue, Liang, Blythe, Emily E, Freiberger, Elyse C, Mamrosh, Jennifer L, Hebert, Alexander S, Reitsma, Justin M, Hess, Sonja, Coon, Joshua J, Deshaies, Raymond J

“…Protein quality control (PQC) plays an important role in stemming neurodegenerative diseases and is essential for the growth of some cancers…”
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Detailed analysis of the plasma extracellular vesicle proteome after separation from lipoproteins by Karimi, Nasibeh, Cvjetkovic, Aleksander, Jang, Su Chul, Crescitelli, Rossella, Hosseinpour Feizi, Mohammad Ali, Nieuwland, Rienk, Lötvall, Jan, Lässer, Cecilia

“… Plasma and serum were collected from healthy subjects, and EVs were isolated by size-exclusion chromatography (SEC…”
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Fifth generation troponin T assay is subject to antibody interference by Hedley, Jeffrey, Menon, Venu, Cho, Leslie, McShane, Adam J.

“…•Fourth and 5th generation troponin T are not subject to the same heterophilic antibody interference…”
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Adiponectin oligomers as potential indicators of adipose tissue improvement in obese subjects by De Rosa, Anna, Monaco, Maria Ludovica, Capasso, Mario, Forestieri, Pietro, Pilone, Vincenzo, Nardelli, Carmela, Buono, Pasqualina, Daniele, Aurora

“…Adiponectin is an adipocytokine that exerts beneficial effects on obesity and related disorders by two receptors (ADIPORs). Adiponectin is produced as a…”
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Isolation of extracellular vesicles: Determining the correct approach (Review) by SZATANEK, RAFAL, BARAN, JAREK, SIEDLAR, MACIEJ, BAJ-KRZYWORZEKA, MONIKA

“… Although vast knowledge on the subject of EVs has accumulated over the years…”
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Soluble amyloid beta-protein dimers isolated from Alzheimer cortex directly induce Tau hyperphosphorylation and neuritic degeneration by Jin, Ming, Shepardson, Nina, Yang, Ting, Chen, Gang, Walsh, Dominic, Selkoe, Dennis J

“…Alzheimer disease is a major cause of cognitive failure, and a pathogenically related but more subtle process accounts for many cases of mild memory symptoms…”
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Oat β-glucan increases postprandial cholecystokinin levels, decreases insulin response and extends subjective satiety in overweight subjects by Beck, Eleanor J, Tosh, Susan M, Batterham, Marijka J, Tapsell, Linda C, Huang, Xu-Feng

“… Seven male and seven female subjects (BMI 25-36 kg/m) consumed five breakfasts (different doses of β-glucan sourced from two different technological processes…”
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Size-Exclusion Chromatography Combined with Ultrafiltration Efficiently Isolates Extracellular Vesicles from Human Blood Samples in Health and Disease by Franco, Chiara, Ghirardello, Anna, Bertazza, Loris, Gasparotto, Michela, Zanatta, Elisabetta, Iaccarino, Luca, Valadi, Hadi, Doria, Andrea, Gatto, Mariele

“…There is still a need for an efficient method for the isolation of extracellular vesicles (EVs) from human blood that provides a reliable yield with acceptable…”
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An Online Buffer Exchange Platform for Charge Detection Mass Spectrometry Analysis of AAVs and AAV-Antibody Complexes by Du, Chen, Cotham, Victoria C, Thakur, Garima, Mink, Sheldon, Tustian, Andrew D, Møller-Tank, Sven, Wang, Shunhai, Li, Ning

“…Adeno-associated viruses (AAVs) are leading vectors in gene therapy that have demonstrated great potential in combating a wide range of human diseases. To…”
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Controlling Actuation Performance in Physically Cross-Linked Polylactone Blends Using Polylactide Stereocomplexation by Izraylit, Victor, Gould, Oliver E C, Rudolph, Tobias, Kratz, Karl, Lendlein, Andreas

“… diversity of polymeric actuator materials. The subject of the present study is a multiblock copolymer with semicrystalline poly(l-lactide) and poly(ε-caprolactone) (PLLA-PCL) segments…”
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Analytical, biochemical and clearance considerations of soluble urokinase plasminogen activator receptor (suPAR) in healthy individuals by Chew-Harris, Janice, Appleby, Sarah, Richards, A. Mark, Troughton, Richard W., Pemberton, Christopher J.

“… The identity of immunoreactive suPAR was confirmed by size-exclusion HPLC. To establish anatomical sites of release and uptake, we measured suPAR in regional samples from subjects undergoing cardiac catheterization…”
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Interaction of the cyclic-di-GMP binding protein FimX and the Type 4 pilus assembly ATPase promotes pilus assembly by Jain, Ruchi, Sliusarenko, Oleksii, Kazmierczak, Barbara I.

“…Type IVa pili (T4P) are bacterial surface structures that enable motility, adhesion, biofilm formation and virulence. T4P are assembled by nanomachines that…”
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A glutaminyl cyclase-catalyzed α-synuclein modification identified in human synucleinopathies by Hartlage-Rübsamen, Maike, Bluhm, Alexandra, Moceri, Sandra, Machner, Lisa, Köppen, Janett, Schenk, Mathias, Hilbrich, Isabel, Holzer, Max, Weidenfeller, Martin, Richter, Franziska, Coras, Roland, Serrano, Geidy E., Beach, Thomas G., Schilling, Stephan, von Hörsten, Stephan, Xiang, Wei, Schulze, Anja, Roßner, Steffen

“…Parkinson’s disease (PD) is a progressive neurodegenerative disorder that is neuropathologically characterized by degeneration of dopaminergic neurons of the…”
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Using two-dimensional correlation size exclusion chromatography (2D-CoSEC) to explore the size-dependent heterogeneity of humic substances for copper binding by Lee, Yun-Kyung, Hur, Jin

“…) is crucial for the systems where the HS MW is subject to change. In this study, two dimensional correlation spectroscopy combined with size exclusion chromatography (2D-CoSEC…”
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Amylin proprotein processing generates progressively more amyloidogenic peptides that initially sample the helical state by Yonemoto, Isaac T, Kroon, Gerard J A, Dyson, H Jane, Balch, William E, Kelly, Jeffery W

“… processing events in the immature secretory granule. Human amylin forms both intracellular and extracellular amyloid deposits in the pancreas of most type II diabetic subjects, likely reflecting compromised secretory cell function…”
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Solution equilibria of cytosine- and guanine-rich sequences near the promoter region of the n-myc gene that contain stable hairpins within lateral loops by Benabou, Sanae, Ferreira, Rubén, Aviñó, Anna, González, Carlos, Lyonnais, Sébastien, Solà, Maria, Eritja, Ramon, Jaumot, Joaquim, Gargallo, Raimundo

“…Cytosine- and guanine-rich regions of DNA are capable of forming complex structures named i-motifs and G-quadruplexes, respectively. In the present study the…”
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A new on-line dual enzymatic method for simultaneous quantification of cholesterol and triglycerides in lipoproteins by HPLC by Usui, Shinichi, Hara, Yukichi, Hosaki, Seijin, Okazaki, Mitsuyo

“… by analyzing serum samples from human and nonhuman subjects. Both TSK and Superose columns produced good within-day imprecision values less than 4.7…”
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In vitro neurotoxicity of salsolinol is attenuated by the presynaptic protein α-synuclein by do Carmo-Gonçalves, Phelippe, Coelho-Cerqueira, Eduardo, Cortines, Juliana R., de Souza, Theo Luiz Ferraz, Romão, Luciana, Follmer, Cristian

“…Salsolinol (SALSO), a product from the reaction of dopamine (DA) with acetaldehyde, is found increased in dopaminergic neurons of Parkinson's disease (PD)…”
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Structural analysis of human Orc6 protein reveals a homology with transcription factor TFIIB by Liu, Shixuan, Balasov, Maxim, Wang, Hongfei, Wu, Lijie, Chesnokov, Igor N, Liu, Yingfang

“…The Origin Recognition Complex (ORC) is a six-subunit protein important for the initiation of DNA replication in eukaryotic cells. Orc6 is the smallest and the…”
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