Suchergebnisse - Enzymologic Humans Reverse Transcriptase Polymerase Chain Reaction/methods Transcription

Flow cytometry isolation and reverse transcriptase-polymerase chain reaction characterization of human round spermatids in infertile patients von Ziyyat, A, Lassalle, B, Testart, J, Briot, P, Amar, E, Finaz, C, Lefèvre, A

“… Flow cytometry coupled to cell sorting is proposed as a method to isolate round spermatids from testicular biopsies in obstructive azoospermic patients. The …”
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Comparison of real-time reverse transcriptase–polymerase chain reaction and nested or commercial reverse transcriptase–polymerase chain reaction for the detection of hepatitis E virus particle in human serum von Ahn, Jeong-min, Rayamajhi, Nabin, Gyun Kang, Sang, Sang Yoo, Han

“… The HEV infection has been diagnosed by detection of anti-HEV antibodies or virus by using reverse transcriptase …”
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Comparison of real-time reverse transcriptase–polymerase chain reaction and nested or commercial reverse transcriptase–polymerase chain reaction for the detection of hepatitis E virus particle in human serum [Diagnostic Microbiology and Infectious Disease 56, 269–274, 2006] von Ahn, Jeong-min, Rayamajhi, Nabin, Gyun Kang, Sang, Sang Yoo, Han

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Human immunodeficiency virus type 1 RNA Levels in different regions of human brain: Quantification using real-time reverse transcriptase-polymerase chain reaction von Kumar, Adarsh M, Borodowsky, Irina, Fernandez, Benny, Gonzalez, Louis, Kumar, Mahendra

“… Human immunodeficiency virus type 1 (HIV-1) enters the central nervous system shortly after the infection and becomes localized in different regions …”
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Prolonged measles virus shedding in human immunodeficiency virus-infected children, detected by reverse transcriptase-polymerase chain reaction von Permar, S R, Moss, W J, Ryon, J J, Monze, M, Cutts, F, Quinn, T C, Griffin, D E

“… A reverse transcriptase-polymerase chain reaction assay was used to detect measles virus RNA in peripheral blood mononuclear cells, urine, and nasopharyngeal specimens from Zambian children …”
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Control genes for reverse transcriptase/polymerase chain reaction (RT-PCR) von Taylor, J J, Heasman, P A

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Messenger RNA differential display reverse‐transcriptase‐polymerase‐chain‐reaction analysis of a progestogen‐suppressive gene in a human endometrial‐cancer cell line von Sakata, Masahiro, Kurachi, Hirohisa, Morishige, Ken‐ichirou, Ogura, Kazuhiro, Yamaguchi, Masaaki, Nishio, Yukihiro, Ikegami, Hiromasa, Miyake, Akira, Murata, Yuji

“… We characterized the progestogen‐induced alterations of gene expression in a human endometrial‐cancer cell line using a mRNA differential‐display reverse‐transcriptase‐polymerase‐chain‐reaction (DDRT‐PCR) method. After 5 …”
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Reverse transcriptase-polymerase chain reaction analysis of thyrocyte-relevant genes in fine-needle aspiration biopsies of the human thyroid von Winzer, R, Schmutzler, C, Jakobs, T C, Ebert, R, Rendl, J, Reiners, C, Jakob, F, Köhrle, J

“… We adapted reverse transcriptase polymerase chain reaction (RT-PCR) to amplify thyroid-typical mRNAs in samples of thyroid carcinoma cells as small as those obtained by fine-needle aspiration biopsy (FNAB …”
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Detection of human 11β-hydroxysteroid dehydrogenase isoforms using reverse-transcriptase-polymerase chain reaction and localization of the type 2 isoform to renal collecting ducts von Whorwood, C.B., Mason, J.I., Ricketts, M.L., Howie, A.J., Stewart, P.M.

“… (type 2) unidirectional dehydrogenase. Using the reverse-transcriptase polymerase chain reaction (RT-PCR …”
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Detection of circulating prostate cells by reverse transcriptase-polymerase chain reaction of human glandular kallikrein (hK2) and prostate-specific antigen (PSA) Messages von Corey, Eva, Arfman, Edward W., Oswin, Matthew M., Melchior, Sebastian W., Tindall, Donald J., Young, Charles Y.-F., Ellis, William J., Vessella, Robert L.

“… To investigate the clinical value of human glandular kallikrein (hK2) reverse transcriptase-polymerase chain reaction (RT-PCR …”
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Detection of cytokine mRNA in human, articular cartilage from patients with rheumatoid arthritis and osteoarthritis by reverse transcriptase-polymerase chain reaction von L Leistad, M Østensen, A Faxvaag

“… ) and osteoarthritis (OA), by means of the reverse transcriptase-polymerase chain reaction (RT-PCR). Message RNA (mRNA) was extracted from fresh or frozen cartilage …”
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Decreased elastin gene expression in noncompliant human bladder tissue: a competitive reverse transcriptase-polymerase chain reaction analysis von Djavan, B, Lin, V, Kaplan, E P, Richier, J C, Shariat, S, Marberger, M, McConnell, J D

“… Using a reverse transcriptase-polymerase chain reaction quantitative technique elastin mRNA can be reliably measured in 5 to 8 mg …”
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The expression of prostate stem cell antigen in human clear cell renal cell carcinoma: a quantitative reverse transcriptase‐polymerase chain reaction analysis von ELSAMMAN, ESSAM M., FUKUMORI, TOMOHARU, TANIMOTO, SHUJI, NAKANISHI, RYOICHI, TAKAHASHI, MASAYUKI, TOIDA, KAZUNORI, KANAYAMA, HIRO‐OMI

“… (81 from CC‐RCC, 73 from normal kidney) using real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR …”
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Competitive reverse transcriptase-polymerase chain reaction shows that dietary zinc supplementation in humans increases monocyte metallothionein mRNA levels von Sullivan, V K, Cousins, R J

“… We used semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to establish that MT mRNA is increased in a human monocytic cell line by addition of zinc to the medium …”
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Prostate-specific antigen mRNA and protein levels in laser microdissected cells of human prostate measured by real-time reverse transcriptase–quantitative polymerase chain reaction and immuno–quantitative polymerase chain reaction von Pinzani, Pamela, Lind, Kristina, Malentacchi, Francesca, Nesi, Gabriella, Salvianti, Francesca, Villari, Donata, Kubista, Mikael, Pazzagli, Mario, Orlando, Claudio

“… Cancer and normal cells selected by laser-assisted microdissection have frequently been used for mRNA expression studies usually by reverse transcriptase …”
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Analysis of human telomerase reverse transcriptase mRNA (hTERT) expression in myxoid liposarcomas using LightCycler real-time quantitative reverse transcriptase-polymerase chain reaction von Schneider-Stock, Regine, Emrich, Thomas, Peters, Brigitte, Jaeger, Viola, Roessner, Albert

“… We describe a convenient, nonradioactive reverse transcription – polymerase chain reaktion (RT‐PCR …”
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Quantitative analysis of neutralizing immune responses to human parvovirus B19 using a novel reverse transcriptase-polymerase chain reaction-based assay von Bostic, J R, Brown, K E, Young, N S, Koenig, S

“… A novel in vitro test for parvovirus neutralization has been developed using reverse transcriptase-polymerase chain reaction to detect viral transcripts in a B19-permissive cell line …”
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Amplification of human beta-actin gene by the reverse transcriptase-polymerase chain reaction: implications for assessment of RNA from formalin-fixed, paraffin-embedded material von Dakhama, A, Macek, V, Hogg, J C, Hegele, R G

“… The polymerase chain reaction (PCR) is a powerful method that allows enzymatic amplification of rate target nucleic acid sequences …”
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The stress kit: a new method based on competitive reverse transcriptase–polymerase chain reaction to quantify the expression of human αB-crystallin, Hsp27, and Hsp60 von Bajramović, Jeffrey J., Geutskens, Sacha B., Bsibsi, Malika, Boot, Marit, Hassankhan, Ryan, Verhulst, Karien C., van Noort, Johannes M.

“… We describe a reverse transcriptase–polymerase chain reaction method for the semiquantitative detection of mRNAs encoding the human heat shock proteins α …”
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Reverse transcriptase—polymerase chain reaction (RT—PCR): a sensitive method to examine basic fibroblast growth factor-induced expression of the early growth response gene-1 ( egr-1) in human umbilical arterial endothelial cells von Ko, Yon, Totzke, Gudrun, Schiermeyer, Beate, Zeitler, Heike, Schmitz, Udo, Vetter, Hans, Sachinidis, Agapios

“… )-mRNA expression in human umbilical arterial endothelial cells (HUAEC). The detection of this gene in HUAEC was performed by Northern blotting and by reverse transcriptase-polymerase chain reaction (RT-PCR …”
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