Basic Concepts in Clinical Biochemistry: A Practical Guide

This book is a practical guidebook in biochemistry, for medical as well as life sciences' students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen...

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Bibliographic Details
Main Author: Kumar, Vijay (Author)
Format: Electronic eBook
Language:English
Published: Singapore : Springer Singapore , 2018.
Edition:1st ed. 2018.
Subjects:
ISBN:9789811081866
Online Access: Get full text
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Table of Contents:
  • Common clinical laboratory hazards and waste disposal
  • Blood collection and preservation
  • Quality Control in laboratory
  • Automation in clinical laboratory
  • Photometry-Colorimeter and Spectrophotometer
  • Preparation of general laboratory solutions and buffers
  • Examination of urine for normal constituents
  • To perform qualitative tests for urinary proteins
  • To determine the quantity of proteins in urine sample using Biuret reaction
  • To estimate the amount of total protein and albumin in serum and to find A/G ratio
  • To perform qualitative test for reducing substances in urine
  • Quantitative analysis of reducing sugars in urine
  • Estimation of blood glucose levels by Glucose Oxidase method
  • Estimation of blood glucose levels by Folin and Wu method
  • To perform Glucose Tolerance Test
  • Estimation of urea in serum and urine
  • To determine urea clearance
  • To estimate creatinine level in serum and urine by Jaffe's reaction
  • To determine creatinine clearance
  • To determine the uric acid concentration in serum and urine
  • Estimation of total calcium in serum and urine
  • Estimation of inorganic phosphorus in serum and urine
  • To estimate the amount of total cholesterol in serum
  • To estimate total and direct bilirubin in serum
  • To determine Alanine and Aspartate Transaminases activity in serum
  • To estimate the activity of alkaline phosphatase in serum
  • To estimate the activity of acid phosphatase in serum
  • To determine serum and urinary amylase activity
  • To estimate the activity of lipase in serum
  • Qualitative analysis of ketone bodies in urine
  • Qualitative test for bile pigments and urobilinogen in urine
  • Determination of total lactate dehydrogenase activity in serum
  • To measure the activity of Creatine kinase-MB and Total Creatine kinase in serum
  • Analysis of Cerebrospinal Fluid for proteins and sugars
  • To analyze lipid profile from given serum sample
  • To determine sodium and potassium in serum by using Flame photometry
  • To perform Radioimmunoassay
  • To perform Enzyme linked Immunosorbant assay
  • Some important case studies.