Advancing Development of Synthetic Gene Regulators With the Power of High-Throughput Sequencing in Chemical Biology /
This book focuses on an "outside the box" notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive go...
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| Format: | Elektronisch E-Book |
| Sprache: | Englisch |
| Veröffentlicht: |
Singapore :
Springer Singapore ,
2018.
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| Ausgabe: | 1st ed. 2018. |
| Schriftenreihe: | Springer Theses, Recognizing Outstanding Ph.D. Research,
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| Schlagworte: | |
| ISBN: | 9789811065477 |
| ISSN: | 2190-5053 |
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| 020 | |a 9789811065477 | ||
| 024 | 7 | |a 10.1007/978-981-10-6547-7 |2 doi | |
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| 040 | |a Springer-Nature |b eng |c CVTISR |e AACR2 | ||
| 041 | |a eng | ||
| 100 | 1 | |a Chandran, Anandhakumar. |4 aut | |
| 245 | 1 | 0 | |a Advancing Development of Synthetic Gene Regulators |h [electronic resource] : |b With the Power of High-Throughput Sequencing in Chemical Biology / |c by Anandhakumar Chandran. |
| 250 | |a 1st ed. 2018. | ||
| 260 | 1 | |a Singapore : |b Springer Singapore , |c 2018. | |
| 300 | |a XV, 114 p. 49 illus., 44 illus. in color. |b online resource. | ||
| 490 | 1 | |a Springer Theses, Recognizing Outstanding Ph.D. Research, |x 2190-5053 | |
| 500 | |a Chemistry and Materials Science | ||
| 505 | 0 | |a Overview of Next-Generation Sequencing Technologies and its application in Chemical Biology -- Next Generation Sequencing Studies Guide the Design of Pyrrole-Imidazole Polyamides with Improved Binding Specificity by the Addition of β-alanine -- Genome-Wide Assessment of the Binding Effects of Artificial Transcriptional Activators by Utilizing the Power of High-Throughput Sequencing -- Deciphering the genomic targets of alkylating polyamide conjugates using high-throughput sequencing. | |
| 516 | |a text file PDF | ||
| 520 | |a This book focuses on an "outside the box" notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)-N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove. First, a cost-effective NGS (ion torrent platform)-based Bind-n-Seq was developed to identify the binding specificity of PIP conjugates in a randomized DNA library. Their biological influences rely primarily on selective DNA binding affinity, so it is important to analyze their genome-wide binding preferences. However, it is demanding to enrich specifically the small-molecule-bound DNA without chemical cross-linking or covalent binding in chromatinized genomes. Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome. SAHA-PIPs binding motifs were identified and the genome-level mapping of SAHA-PIPs-enriched regions provided evidence for the differential activation of the gene network. A method using high-throughput sequencing to map the binding sites and relative enriched regions of alkylating PIP throughout the human genome was also developed. The genome-level mapping of alkylating the PIP-enriched region and the binding sites on the human genome identifies significant genomic targets of breast cancer. It is anticipated that this pioneering low-cost, high through-put investigation at the sequence-specific level will be helpful in understanding the binding specificity of various DNA-binding small molecules, which in turn will be beneficial for the development of small-molecule-based drugs targeting a genome-level sequence. . | ||
| 650 | 0 | |a Biotechnology. | |
| 650 | 0 | |a Gene therapy. | |
| 650 | 0 | |a Bioorganic chemistry. | |
| 856 | 4 | 0 | |u http://hanproxy.cvtisr.sk/han/cvti-ebook-springer-eisbn-978-981-10-6547-7 |y Vzdialený prístup pre registrovaných používateľov |
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| 919 | |a 978-981-10-6547-7 | ||
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| 999 | |c 272319 |d 272319 | ||